Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,341,588 A→C 12.0% K232T (AAG→ACG)  panB → 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,341,5880AC12.0% 135.0 / 11.3 50K232T (AAG→ACG) panB3‑methyl‑2‑oxobutanoate hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base A (24/20);  new base C (2/4);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.43e-01

TACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTGCGCGCGGCAGTGGC  >  NC_002947/5341441‑5341734
                                                                                                                                                   |                                                                                                                                                  
tACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTgaag                                                                                                                                                   <  7:80382/149‑1 (MQ=255)
     aGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACtt                                                                                                                                              >  8:76507/1‑149 (MQ=255)
       cGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCTTCACGAACTTcg                                                                                                                                            <  5:313983/149‑2 (MQ=255)
       cGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCTTCACGAACTTcg                                                                                                                                            <  4:69018/149‑2 (MQ=255)
        gAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCTTCACGAACTTcgg                                                                                                                                           <  7:238156/149‑3 (MQ=255)
                        tCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGat                                                                                                                           <  5:893/149‑1 (MQ=255)
                         cACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGata                                                                                                                          >  2:55869/1‑149 (MQ=255)
                               tGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATa                                                                                                                    <  7:237206/149‑1 (MQ=255)
                                  cGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAgcg                                                                                                                 >  7:146520/1‑149 (MQ=255)
                                  cGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAgcg                                                                                                                 >  8:69968/1‑149 (MQ=255)
                                         aTCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCtcgt                                                                                                          <  8:146520/149‑1 (MQ=255)
                                          tCCCGGTAATCGGCATTGGCGCCGGCAGCTCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTCAGCGGCCGGGTACCGAAGTTCGTTAACAACTTCATGCACGGCCAACCGGATATCCATAGCGCCCtcgtc                                                                                                         >  6:3677/1‑149 (MQ=255)
                                          tCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCTTCACGAACtt                                                                                                                                              >  2:299009/1‑112 (MQ=255)
                                          tCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCTTCACGAACtt                                                                                                                                              <  1:299009/112‑1 (MQ=255)
                                          tCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCtcgtc                                                                                                         >  1:69132/1‑149 (MQ=255)
                                            ccGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGata                                                                                                                          >  8:189714/1‑130 (MQ=255)
                                            ccGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGata                                                                                                                          <  7:189714/130‑1 (MQ=255)
                                              ggTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGcc                                                                                                               <  5:45578/139‑1 (MQ=255)
                                              ggTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGcc                                                                                                               >  6:45578/1‑139 (MQ=255)
                                               gTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTa                                                                                                    >  4:94679/1‑149 (MQ=255)
                                                     ggCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCg                                                                                               >  1:270067/1‑148 (MQ=255)
                                                      tcggtGGCGCTGCCGGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAg                                                                                             >  5:279856/5‑149 (MQ=255)
                                                       cATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAgg                                                                                            <  3:115001/149‑1 (MQ=255)
                                                           ggCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCgg                                                                                         <  7:76507/148‑1 (MQ=255)
                                                                cGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTcaagc                                                                                   <  1:55869/149‑1 (MQ=255)
                                                                      cgcCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTTGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGt                                                                               >  8:220432/1‑147 (MQ=255)
                                                                        ccACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGt                                                                           >  5:44983/1‑149 (MQ=255)
                                                                        ccACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGt                                                                           >  1:264497/1‑149 (MQ=255)
                                                                              aTGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCa                                                                     <  2:69132/149‑1 (MQ=255)
                                                                              aTGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCa                                                                     <  6:279856/149‑1 (MQ=255)
                                                                                     ggtactggtactGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGc                                                               >  6:64223/1‑148 (MQ=255)
                                                                                            gtactgCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAacac                                                         <  7:69968/147‑1 (MQ=255)
                                                                                               ctgCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGtt                                                    <  2:270067/149‑1 (MQ=255)
                                                                                                      aCATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCa                                             <  3:94679/149‑1 (MQ=255)
                                                                                                        aTGCTCGGCCTGTCGCTTAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATg                                           <  7:291747/149‑1 (MQ=255)
                                                                                                        aTGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCAt                                            >  4:17489/1‑148 (MQ=255)
                                                                                                           cTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGtt                                                    <  5:201978/137‑1 (MQ=255)
                                                                                                           cTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGtt                                                    >  6:201978/1‑137 (MQ=255)
                                                                                                               gCCTGTCGCTGAGCGGCGGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCGAACCGGATATCCATAGCTCCCTCGTCGGTTACGTCGAGGCGGTCAAGCATGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAg                                    <  5:3677/149‑1 (MQ=255)
                                                                                                                  tGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGTc                                  >  5:69587/1‑148 (MQ=255)
                                                                                                                    tCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGACCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTcaa                                                                                     >  7:253866/1‑95 (MQ=255)
                                                                                                                    tCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGACCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTcaa                                                                                     <  8:253866/95‑1 (MQ=255)
                                                                                                                      gCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGTCAAGa                              <  5:64223/148‑1 (MQ=255)
                                                                                                                            cGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTGCAGTGCATGAATACAGTCAAGACCGTCcg                       >  5:282792/1‑149 (MQ=255)
                                                                                                                                  ggTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACt                 >  2:211700/1‑149 (MQ=255)
                                                                                                                                    tACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGc                                                                                           <  5:14054/73‑1 (MQ=255)
                                                                                                                                    tACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGc                                                                                           >  6:14054/1‑73 (MQ=255)
                                                                                                                                      ccGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTgcgc             >  5:124579/1‑149 (MQ=255)
                                                                                                                                      atgAAGTTCTTCACGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTgcgc             >  3:196294/3‑149 (MQ=255)
                                                                                                                                             tCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTGCGCGCGGCa       <  3:17489/148‑1 (MQ=255)
                                                                                                                                              cGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTGCGCGCGGCAGt     >  4:304053/1‑149 (MQ=255)
                                                                                                                                                  aagaaCTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTGCGCGCGGCAGTGgc  <  7:220432/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTGCGCGCGGCAGTGGC  >  NC_002947/5341441‑5341734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑