Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,354,033 G→A 7.4% G28G (GGC→GGT)  pnp ← polyribonucleotide nucleotidyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,354,0330GA 7.4% 157.8 / 2.3 54G28G (GGC→GGT) pnppolyribonucleotide nucleotidyltransferase
Reads supporting (aligned to +/- strand):  ref base G (27/23);  new base A (2/2);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

GAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCTTTTTCAGTGGCTCTTGGGGGAACTGGTTTCTTGCGAATTCTTGGGCAGAACGG  >  NC_002947/5353886‑5354181
                                                                                                                                                   |                                                                                                                                                      
gaagCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGgcgc                                                                                                                                                       >  7:48631/1‑149 (MQ=255)
    ccACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGt                                                                                                                                                   <  6:189405/149‑1 (MQ=255)
          gggATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTg                                                                                                                                             <  8:48631/149‑1 (MQ=255)
                    cAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATa                                                                                                                                   >  5:321880/1‑149 (MQ=255)
                    cAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCACCGGTTGCCTGACGGGCAATa                                                                                                                                   >  7:158221/1‑149 (MQ=255)
                    cAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCACCGGTTGCCTGACGGGCAATa                                                                                                                                   >  7:153741/1‑149 (MQ=255)
                                  ttttCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTc                                                                                                                        >  6:131926/1‑146 (MQ=255)
                                  ttttCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTc                                                                                                                        >  8:246720/1‑146 (MQ=255)
                                  ttttCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTc                                                                                                                        >  1:289336/1‑146 (MQ=255)
                                        tGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGAc                                                                                                               >  5:108527/1‑149 (MQ=255)
                                             gTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑c                                                                                                         >  7:228637/1‑148 (MQ=255)
                                                aaCCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGAtt                                                                                                     >  1:270108/1‑149 (MQ=255)
                                                          gggAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTg                                                                                           >  2:48586/1‑149 (MQ=255)
                                                            gaagaaACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCgaga                                                                                                                    >  6:179742/1‑124 (MQ=255)
                                                            gaagaaACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCgaga                                                                                                                    <  5:179742/124‑1 (MQ=255)
                                                            gaagaaACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGCTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGC‑T‑CGATTGACCGAACTgg                                                                                          >  2:36173/1‑148 (MQ=255)
                                                              agaaACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGaaa                                                                                       <  6:321880/149‑1 (MQ=255)
                                                              agaaACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGaaa                                                                                       <  5:131926/149‑1 (MQ=255)
                                                              agaaACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGa                                                                                         >  5:146322/1‑147 (MQ=255)
                                                                  aCCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCgaga                                                                                                                    >  5:207131/1‑118 (MQ=255)
                                                                  aCCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCgaga                                                                                                                    <  6:207131/118‑1 (MQ=255)
                                                                   cccTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCACCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCtt                                                                                  <  8:158221/149‑1 (MQ=255)
                                                                   cccTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCACCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCtt                                                                                  <  8:153741/149‑1 (MQ=255)
                                                                             gATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCgg                                                                         <  6:108527/148‑1 (MQ=255)
                                                                             gATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCgg                                                                         <  7:246720/148‑1 (MQ=255)
                                                                                      gTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTATCGGGTTCACGGtt                                                               >  2:98217/1‑149 (MQ=255)
                                                                                       tttGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGtt                                                               <  2:289336/148‑1 (MQ=255)
                                                                                            cgcCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGtt                                                               <  7:124594/143‑1 (MQ=255)
                                                                                            cgcCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGtt                                                               >  8:124594/1‑143 (MQ=255)
                                                                                            cgcCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTAc                                                         <  4:47695/149‑1 (MQ=255)
                                                                                                tACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTcc                                                            >  5:79404/1‑142 (MQ=255)
                                                                                                tACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTcc                                                            <  6:79404/142‑1 (MQ=255)
                                                                                                  ccACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCttttt                                                   <  7:182979/149‑1 (MQ=255)
                                                                                                  ccACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCttttt                                                   <  2:270108/149‑1 (MQ=255)
                                                                                                     caGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCTTTTTCAg                                                <  1:48586/149‑1 (MQ=255)
                                                                                                          aCCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCTTTTTCAGTGGct                                           >  5:186221/1‑149 (MQ=255)
                                                                                                           ccAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCTTTTTCAGTGGctc                                          >  3:186600/1‑149 (MQ=255)
                                                                                                             aGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGCCCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCTTTTTCAGTGGCTCtt                                        >  7:55400/1‑149 (MQ=255)
                                                                                                                    tGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCTTTTTCAGTGGCTCTTGGGGGaa                                 >  6:264300/1‑149 (MQ=255)
                                                                                                                     gACGTCGTTGTCGACGGTAACCAGCACGGCGGCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGGCTCCGATTGACCGAACTGGAAAGTCTTGATTATCGGGTTCACGGTTTCCTACCTTTTTCAGTGGCTCTTggggg                                   <  1:98217/148‑1 (MQ=255)
                                                                                                                      aCGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCTTTTTCAGTGGCTCTTGGGGGAAc                                <  4:186600/148‑1 (MQ=255)
                                                                                                                        gtcgtTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCTTTTTCAGTGGCTCTTGGGGGAACTgg                             >  2:248394/1‑149 (MQ=255)
                                                                                                                        gtcgtTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCTTTTTCAGTGGCTCTTGGGGGAACTgg                             >  7:119574/1‑149 (MQ=255)
                                                                                                                               tCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCTTTTTCAGTGGCTCTTGGGGGAACTGGTTTCtt                       <  8:119574/148‑1 (MQ=255)
                                                                                                                                 gACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCTTTTTCAGTGGCTCTTGGGGGAACTGGTTTCTTGc                     >  1:92693/1‑148 (MQ=255)
                                                                                                                                  aCGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCTTTTTCAGTGGCTCTTGGGGGAACTGGTTTCTTGCg                    >  3:62483/1‑148 (MQ=255)
                                                                                                                                      tAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCTTTTTCAGTGGCTCTTGGGGGAACTGGTTTCTTGCGAATTc               >  1:205305/1‑149 (MQ=255)
                                                                                                                                        aCCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCTTTTTCAGTGGCTCTTGGGGGAACTGGTTTCTTGCGAATTCtt             <  8:228637/149‑1 (MQ=255)
                                                                                                                                        aCCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCTTTTTCAGTGGCTCTTGGGGGAACTGGTTTCTTGCGAATTCtt             <  1:248394/149‑1 (MQ=255)
                                                                                                                                         ccAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGtt                                                               <  7:271409/98‑1 (MQ=255)
                                                                                                                                         ccAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGtt                                                               >  8:271409/1‑98 (MQ=255)
                                                                                                                                         ccAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCTTTTTCAGTGGCTCTTGGGGGAACTGGTTTCTTGCGAATTCTTg            >  6:138477/1‑149 (MQ=255)
                                                                                                                                          cAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCTAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCTTTTTCAGTGGCTCTTGGGGGAACTGGTTTCTTGCGAATTCTTgg           <  6:158367/149‑1 (MQ=255)
                                                                                                                                           aGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCTTTTTCAGTGGCTCTTGGGGGAACTGGTTTCTTGCGAATTCTTg            <  6:34443/147‑1 (MQ=255)
                                                                                                                                           aGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCTTTTTCAGTGGCTCTTGGGGGAACTGGTTTCTTGCGAATTCTTg            >  5:34443/1‑147 (MQ=255)
                                                                                                                                                   gcCGGTTGCCTGACGGGCAATACGGCCCGTTTCGATATTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCTTTTTCAGTGGCTCTTGGGGGAACTGGTTTCTTGCGAATTCTTGGTCAGAACgg  <  1:36173/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
GAAGCCACCTGGGATCTTGCCAGCGGCGTAGGTCTTTTCCTGGTAGTGAACCGACAGCGGGAAGAAACCCTTGCCTGGATCAGCCTGTTTGGCGCCTACCACAGTCACCAGCACGGTGACGTCGTTGTCGACGGTAACCAGCACGGCGCCGGTTGCCTGACGGGCAATACGGCCCGTTTCGAGAGTGACGG‑T‑CGATTGACCGAACTGGAAAGTCTTGATTACCGGGTTCACGGTTTCCTACCTTTTTCAGTGGCTCTTGGGGGAACTGGTTTCTTGCGAATTCTTGGGCAGAACGG  >  NC_002947/5353886‑5354181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑