Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,381,269 G→A 9.1% intergenic (+34/+31) bamE → / ← PP_4732 outer membrane protein assembly factor BamE/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,381,2690GA 9.1% 243.2 / 11.1 88intergenic (+34/+31)bamE/PP_4732outer membrane protein assembly factor BamE/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (43/37);  new base A (4/4);  total (47/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.67e-01

TGAAGAAAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGAAGGACAGG  >  NC_002947/5381127‑5381417
                                                                                                                                              |                                                                                                                                                    
tGAAGAAAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCgg                                                                                                                                                >  7:246161/1‑149 (MQ=255)
  aagaaAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCgg                                                                                                                                                <  1:144692/147‑1 (MQ=255)
  aagaaAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGc                                                                                                                                              >  3:166717/1‑149 (MQ=255)
   agaaAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCTATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCt                                                                                                                                             >  3:278140/1‑149 (MQ=255)
    gaaAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCtt                                                                                                                                            <  2:287601/149‑1 (MQ=255)
    gaaAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACCAAGTCCGGGCtt                                                                                                                                            >  5:58433/1‑149 (MQ=255)
       aaaCCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACCAAGTCCGGGCttttt                                                                                                                                         <  6:58433/149‑1 (MQ=255)
        aaCCGGCCAAGCCTGTTTTTAGATAAGAGTCGATGCAGCGCGAGATCTACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACCAAGTCCGGGCtttttt                                                                                                                                        <  2:21546/149‑1 (MQ=255)
        aaCCGGCCAAGCCTGGTTCGACCTAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCtttttt                                                                                                                                        <  6:195475/149‑1 (MQ=255)
        aaCCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCtttttt                                                                                                                                        >  3:245949/1‑149 (MQ=255)
            ggCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTtttgttt                                                                                                                                    <  6:269542/149‑1 (MQ=255)
                aaGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTgcgc                                                                                                                                >  1:145190/1‑149 (MQ=255)
                aaGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTgcgc                                                                                                                                >  1:140419/1‑149 (MQ=255)
                  gCCTGGTTCGACCGAAGAGGCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTTCGCGGGAACATCAGATGCAAATAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCt                                                                                                                              <  1:91215/149‑1 (MQ=255)
                      ggTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCACGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAAAATCAGATGCAAAAAAGCCCGGACTTGTTCCGGGCTTTTTAGTTTGCTCCTtat                                                                                                                           >  4:208226/1‑148 (MQ=255)
                       gTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTtat                                                                                                                           <  4:166717/147‑1 (MQ=255)
                           gACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGt                                                                                                                       >  7:3419/1‑147 (MQ=255)
                             ccGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAgg                                                                                                                   <  4:245949/149‑1 (MQ=255)
                              cGAAGAGTCCATCCAGCGCGACATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCAAAGCGGGAACATCAGATGCAAAAAAGCAACGCCTTGAACCGCGCTAAATAGTTTGCGCCTTATACGTCAgg                                                                                                                   >  4:244850/1‑148 (MQ=255)
                                aaGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCAt                                                                                                                >  3:50596/1‑149 (MQ=255)
                                 agatTCCATCCAGCTCGAGTTCGAGAGAATCGTGAATACCCCGGTCCGGACCCGGGGACCGGTGGATACGCGCTAAGCGGGAACATCAGATGCAAAAATTCCCGGACTTGGTCCGGGCTTTTTTGTTTGGGCCTTATACGTGAGGCAtt                                                                                                               <  4:50596/149‑1 (MQ=255)
                                    gTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTtat                                                                                                                           <  3:148403/134‑1 (MQ=255)
                                    gTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTtat                                                                                                                           >  4:148403/1‑134 (MQ=255)
                                     tccatccaGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAAt                                                                                                           >  2:121808/1‑149 (MQ=255)
                                      ccatccaGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAAt                                                                                                           <  2:145190/148‑1 (MQ=255)
                                      ccatccaGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAAt                                                                                                           <  2:140419/148‑1 (MQ=255)
                                           caGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTg                                                                                                     <  4:278140/149‑1 (MQ=255)
                                           caGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTg                                                                                                     <  3:61899/149‑1 (MQ=255)
                                           caGCGCGAGATCGACACCATCGAGACGACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCtt                                                                                                      <  4:46116/148‑1 (MQ=255)
                                             gcgcGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGTAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGc                                                                                                   <  1:254414/149‑1 (MQ=255)
                                               gcgAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCAc                                                                                                 >  8:292723/1‑149 (MQ=255)
                                                cgAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACg                                                                                                >  1:30035/1‑149 (MQ=255)
                                                 gagaTCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGc                                                                                               >  6:60086/1‑149 (MQ=255)
                                                  agaTCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCt                                                                                              >  7:65452/1‑149 (MQ=255)
                                                     tCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTgg                                                                                            >  6:271290/1‑148 (MQ=255)
                                                     tCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGc                                                                                           <  8:3419/149‑1 (MQ=255)
                                                      cGACACCATCGAGACTACCCCCGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTgg                                                                                            >  3:166778/1‑147 (MQ=255)
                                                        acacCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAg                                                                                         >  5:199673/1‑148 (MQ=255)
                                                          acCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTggtcgatg                                                                                      >  1:33376/1‑143 (MQ=255)
                                                          acCATCGAGACAACCCCGGTCCCGACCCCGGCACGGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAAc                                                                                      <  5:142482/149‑1 (MQ=255)
                                                            cATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCtttttt                                                                                                                                        <  8:70984/97‑1 (MQ=255)
                                                            cATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCtttttt                                                                                                                                        >  7:70984/1‑97 (MQ=255)
                                                            cATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACg                                                                                     >  4:149050/1‑148 (MQ=255)
                                                              tCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATACAAAAAAGCCCGGACCAAGTCCGGGc                                                                                                                                              >  4:43317/1‑89 (MQ=38)
                                                              tCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATACAAAAAAGCCCGGACCAAGTCCGGGc                                                                                                                                              <  3:43317/89‑1 (MQ=38)
                                                                   aCTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAAt                                                                                                           >  6:51598/1‑119 (MQ=255)
                                                                     tACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAg                                                                           >  7:65429/1‑149 (MQ=255)
                                                                     tACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAg                                                                           >  7:184013/1‑149 (MQ=255)
                                                                       ccccGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGc                                                                          <  4:192717/148‑1 (MQ=255)
                                                                         ccGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTa                                                                       >  2:117937/1‑149 (MQ=255)
                                                                           ggTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGt                                                                                                                       >  5:200367/1‑99 (MQ=255)
                                                                           ggTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGt                                                                                                                       <  6:200367/99‑1 (MQ=255)
                                                                             tCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTcg                                                                   <  3:244850/149‑1 (MQ=255)
                                                                               ccGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCg                                                                         >  1:247279/1‑141 (MQ=255)
                                                                               ccGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCg                                                                         <  2:247279/141‑1 (MQ=255)
                                                                                cGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTcgccg                                                                <  2:30035/149‑1 (MQ=255)
                                                                                 gACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTcgccgc                                                               >  1:31837/1‑149 (MQ=255)
                                                                                    cccGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCa                                                                            >  4:220928/1‑133 (MQ=255)
                                                                                    cccGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCa                                                                            <  3:220928/133‑1 (MQ=255)
                                                                                     ccGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGa                                                           >  6:40207/1‑149 (MQ=255)
                                                                                     ccGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGa                                                           <  6:70461/149‑1 (MQ=255)
                                                                                         cACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTc                                                                                                              <  2:7265/94‑1 (MQ=255)
                                                                                         cACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTc                                                                                                              >  1:7265/1‑94 (MQ=255)
                                                                                          aCCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGaa                                                      <  7:292723/149‑1 (MQ=255)
                                                                                           ccGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAAt                                                     <  5:271290/149‑1 (MQ=255)
                                                                                                 gAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCgg                                                <  1:121808/148‑1 (MQ=255)
                                                                                                 gAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCg                                                 <  3:208226/147‑1 (MQ=255)
                                                                                                    aCCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGTCCAGCGTATTCGCCGCCTGATTGAATAGCGGGcc                                             >  1:21897/1‑148 (MQ=255)
                                                                                                      ccGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCaa                                          >  8:62927/1‑149 (MQ=255)
                                                                                                      ccGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCaa                                          >  6:316779/1‑149 (MQ=255)
                                                                                                      ccGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACAAGGTCCGGGCTTTTTAGTTTGCGCCTAATACGTCAGGCATTCAATTGATTCGCACGCTGGCAGAACGCATCGACCAGCGTATTACCCGCCTGATTGAATAGCGCGCCCaa                                          >  5:36777/1‑149 (MQ=255)
                                                                                                               gggAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGgcgcg                                 >  8:20957/1‑149 (MQ=255)
                                                                                                                ggAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTtata                                                                                                                          <  7:208783/59‑1 (MQ=255)
                                                                                                                ggAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTtata                                                                                                                          >  8:208783/1‑59 (MQ=255)
                                                                                                                 gAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGgcgcgc                                >  3:233875/1‑148 (MQ=255)
                                                                                                                 gAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGca                               >  3:229702/1‑149 (MQ=255)
                                                                                                                 gAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGca                               <  7:62927/149‑1 (MQ=255)
                                                                                                                       cAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCg                         >  6:313155/1‑149 (MQ=255)
                                                                                                                        gcaaacAAAAAAGCCCGGACCAAGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCATATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCgg                        >  1:218282/6‑149 (MQ=39)
                                                                                                                        aGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCTCGCACAATCgg                        >  6:258843/1‑149 (MQ=255)
                                                                                                                                   aGCCCGGACCAAGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTaa              >  8:116284/1‑148 (MQ=255)
                                                                                                                                   aGCCCGGACCAAGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCATATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAAt             <  2:218282/149‑1 (MQ=255)
                                                                                                                                       cGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGaa         <  3:149050/149‑1 (MQ=255)
                                                                                                                                       cGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGaa         <  4:166778/149‑1 (MQ=255)
                                                                                                                                        ggACTTGGTCCTGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGAAg        <  8:65452/149‑1 (MQ=255)
                                                                                                                                        ggACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGAAg        <  8:184013/149‑1 (MQ=255)
                                                                                                                                        ggACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGAAg        <  8:65429/149‑1 (MQ=255)
                                                                                                                                            ttGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGAAGGa      >  6:29943/1‑147 (MQ=255)
                                                                                                                                              ggTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGAAGGACAgg  >  8:11223/1‑149 (MQ=255)
                                                                                                                                              ggTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGAAGGACAgg  >  8:6843/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
TGAAGAAAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGAAGGACAGG  >  NC_002947/5381127‑5381417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑