Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,391,825 A→C 6.7% L24R (CTC→CGC) ‡ PP_5709 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,391,8250AC 6.7% 188.1 / 3.8 60L24R (CTC→CGC) ‡PP_5709hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (23/32);  new base C (2/2);  total (25/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ACCGCATCAGGGAGCTTCGTCAGCCCTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCAATAACCTTGCCATGCAAGCA  >  NC_002947/5391678‑5391961
                                                                                                                                                   |                                                                                                                                        
aCCGCATCAGGGAGCTTCGTCAGCCCTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGag                                                                                                                                         >  4:200340/1‑149 (MQ=255)
   gCATCAGGGAGCTTCGTCAGCCCTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGagcag                                                                                                                                      <  4:7338/149‑1 (MQ=255)
    cATCAGGGAGCTTCGTCAGCCCTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGagcagc                                                                                                                                     >  8:232639/1‑149 (MQ=255)
        aGGGAGCTTCGTCAGCCCTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCtt                                                                                                                                 <  2:117001/149‑1 (MQ=255)
        aGGGAGCTTCGTCAGCCCTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCtt                                                                                                                                 <  7:232639/149‑1 (MQ=255)
          ggAGCTTCGTCAGCCCTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCTCGCAGccgtctc                                                                                                                               <  4:142125/149‑6 (MQ=255)
            aGCTTCGTCAGCCCTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGTGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGGCCTTCAGa                                                                                                                             <  6:244582/149‑1 (MQ=255)
            aGCTTCCTCAGCCCTTCTTTCTGCGTCAACATGTAAGCTAGGATGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGa                                                                                                                             <  6:240307/149‑1 (MQ=255)
                  gTCAGCCCTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCtt                                                                                                                       >  3:182174/1‑149 (MQ=255)
                     aGCCCTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCtttt                                                                                                                     <  3:249044/148‑1 (MQ=255)
                     aGCCCTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTg                                                                                                                    <  4:269016/149‑1 (MQ=255)
                         cTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGAtt                                                                                                                >  6:290026/1‑149 (MQ=255)
                             gTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTc                                                                                                             <  8:175743/148‑1 (MQ=255)
                             gTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTc                                                                                                             <  1:311640/148‑1 (MQ=255)
                              ttCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGc                                                                                                           >  3:202481/1‑149 (MQ=255)
                                  gCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCg                                                                                                       >  7:275814/1‑149 (MQ=255)
                                   cGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCt                                                                                                         <  7:232631/146‑1 (MQ=255)
                                   cGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCg                                                                                                       <  8:63159/148‑1 (MQ=255)
                                     tCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTc                                                                                                    >  3:2853/1‑149 (MQ=255)
                                        aCTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTc                                                                                                 <  3:200340/149‑1 (MQ=255)
                                                  tAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGTGCAGCCCTTCAGATGCCTTTTGGATTTTCGcc                                                                                                          <  1:251972/130‑1 (MQ=255)
                                                  tAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGcc                                                                                                          >  2:251972/1‑130 (MQ=255)
                                                     gCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAAt                                                                                    <  4:2853/149‑1 (MQ=255)
                                                       tGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAAtct                                                                                  >  4:140814/1‑149 (MQ=255)
                                                         gCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCg                                                                                                       >  7:236659/1‑126 (MQ=255)
                                                         gCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCg                                                                                                       <  8:236659/126‑1 (MQ=255)
                                                         gCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTc                                                                                                 >  1:209505/1‑132 (MQ=255)
                                                         gCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTc                                                                                                 <  2:209505/132‑1 (MQ=255)
                                                                 ttCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCg                                                                                                       >  4:190222/1‑118 (MQ=255)
                                                                 ttCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCg                                                                                                       <  3:190222/118‑1 (MQ=255)
                                                                   ccTCACCATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGGCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTc                                                                        <  6:907/147‑1 (MQ=255)
                                                                     tCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCa                                                                     >  8:13595/1‑148 (MQ=255)
                                                                       aCTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATa                                                                   <  4:182174/148‑1 (MQ=255)
                                                                       aCTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAg                                                                  >  4:205051/1‑149 (MQ=255)
                                                                                gACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGccc                                                          >  2:14284/1‑148 (MQ=255)
                                                                                gACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCt                                                         <  2:102498/149‑1 (MQ=255)
                                                                                     aaCGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTAt                                                       <  1:76029/146‑1 (MQ=255)
                                                                                     aaCGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTAt                                                       >  2:76029/1‑146 (MQ=255)
                                                                                     aaCGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGAc                                                    >  6:271036/1‑149 (MQ=255)
                                                                                     aaCGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAAACAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGAc                                                    >  3:245848/1‑149 (MQ=255)
                                                                                         gCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGc                                                <  5:290026/149‑1 (MQ=255)
                                                                                          ccATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATGAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCt                                               <  4:202481/149‑1 (MQ=255)
                                                                                           cATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCATTCAGATGCCTTTTGGATTTTCGCATCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCATATGAACGGCt                                               >  7:164973/1‑148 (MQ=255)
                                                                                             tCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACa                                            <  3:140814/149‑1 (MQ=255)
                                                                                             tCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACa                                            >  2:82053/1‑149 (MQ=255)
                                                                                              ccAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACAt                                           >  4:185456/1‑149 (MQ=255)
                                                                                                   gTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGaa                                      <  1:14284/149‑1 (MQ=255)
                                                                                                     gAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCACATCTCGCGAAGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATc                                    >  2:308199/1‑149 (MQ=255)
                                                                                                             tGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGtttt                             <  1:31912/148‑1 (MQ=255)
                                                                                                               ccAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTc                                                                                                 <  6:58256/78‑1 (MQ=255)
                                                                                                               ccAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTc                                                                                                 >  5:58256/1‑78 (MQ=255)
                                                                                                               ccAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCAc                          <  7:13595/149‑1 (MQ=255)
                                                                                                               ccAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCAc                          <  7:176084/149‑1 (MQ=255)
                                                                                                                         tCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCaa                     <  7:108564/144‑1 (MQ=255)
                                                                                                                         tCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCaa                     >  8:108564/1‑144 (MQ=255)
                                                                                                                            aCTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCAATAACCTTg             <  1:82053/149‑1 (MQ=255)
                                                                                                                                      ctgaagggCTGCTCGCAGCCCTTCAGATGCCTTTTGGATTTTAGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCAATAACCTTGCCATGCAAg    >  5:224880/7‑148 (MQ=255)
                                                                                                                                       tgaagggCTGCTCGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCAATAACCTTGCCATGCAAg    >  2:84395/6‑147 (MQ=255)
                                                                                                                                       gagaCGGCTGCGATGAGGCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCAATAACCTTGCCATGCAAGCa  <  4:245848/149‑1 (MQ=255)
                                                                                                                                       gagaCGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCAATAACCTTGCCATGCAAGCa  <  1:308199/149‑1 (MQ=255)
                                                                                                                                         aagggCTGCTCGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCAATAACCTTGCCATGCAAGCa  <  6:224880/144‑1 (MQ=255)
                                                                                                                                             gCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCAATAACCTTGCCATGCAAg    <  6:323103/141‑1 (MQ=255)
                                                                                                                                             gCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCAATAACCTTGCCATGCAAg    >  5:323103/1‑141 (MQ=255)
                                                                                                                                                   |                                                                                                                                        
ACCGCATCAGGGAGCTTCGTCAGCCCTTCGTTCTGCGTCAACTTGTAAGCTAGGCTGGCAACCACTTCCTCACTATCACCGACTAAACGGCCATCCAGAGTGAACGGTTTGCCAGGGTAGGTCGACTGCAACTGGGAGACGGCTGCGAGCAGCCCTTCAGATGCCTTTTGGATTTTCGCCTCGGTCTTCAGGTCAATCAAATCTCGCGATGCTCGCATAGCCTTGCCCTATGACCGGCTACATCAGAATCTTGTTTTCACTTCAATAACCTTGCCATGCAAGCA  >  NC_002947/5391678‑5391961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑