Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 513,453 T→G 11.5% intergenic (‑100/‑91) crp ← / → yhfA DNA‑binding transcriptional dual regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947513,4530TG11.5% 131.6 / 8.7 51intergenic (‑100/‑91)crp/yhfADNA‑binding transcriptional dual regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (21/24);  new base G (3/3);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

CGATGTTCTTTATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGCAATGTTCCTCGGCGAATCGGG  >  NC_002947/513314‑513596
                                                                                                                                           |                                                                                                                                               
cGATGTTCTTTATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTAATTTGGCGACAGCTTATAAGACACTACTAGATGTTTCGGTACt                                                                                                                                         >  8:206600/1‑148 (MQ=255)
      tCTTTATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGa                                                                                                                                  >  3:197403/1‑149 (MQ=255)
       cTTTATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGa                                                                                                                                  >  2:215277/1‑148 (MQ=255)
        tttATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGaca                                                                                                                                >  4:219759/1‑149 (MQ=255)
          tATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGac                                                                                                                                 <  4:197403/146‑1 (MQ=255)
          tATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACACATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGacac                                                                                                                               <  3:252889/148‑1 (MQ=255)
           aTCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGttt                                                                                                                                                <  1:1842/130‑1 (MQ=255)
           aTCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGttt                                                                                                                                                >  2:1842/1‑130 (MQ=255)
                ggCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACAtctc                                                                                                                          <  1:215277/147‑1 (MQ=255)
                 gCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGt                                                                                                                        <  3:219759/148‑1 (MQ=255)
                 gCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACAACGAGATGTGTCGGTTTAGTACCGAAACATCTCGta                                                                                                                       <  8:84847/149‑2 (MQ=38)
                        gggCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTc                                                                                                                 <  2:236173/148‑1 (MQ=255)
                             gAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAg                                                                                                           >  3:279747/1‑149 (MQ=255)
                                   aCCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGca                                                                                                      >  3:299575/1‑148 (MQ=255)
                                   aCCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGATTTTCCCACAGCCGca                                                                                                      >  5:63466/1‑148 (MQ=255)
                                             aaTCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGTTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAg                                                                                           <  1:128993/149‑1 (MQ=255)
                                             aaTCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAg                                                                                           >  1:116997/1‑149 (MQ=255)
                                               tCCTGTTCGATATGACTCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCATTTCCCACAGCCGCACAAGGTCTAAGCt                                                                                         <  1:178143/149‑1 (MQ=255)
                                               tCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCt                                                                                         <  2:116997/149‑1 (MQ=255)
                                                  tGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGa                                                                                       >  7:73157/1‑148 (MQ=255)
                                                       gATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGac                                                                                      >  8:143914/1‑144 (MQ=255)
                                                       gATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGac                                                                                      <  7:143914/144‑1 (MQ=255)
                                                       gATATGACGCATAGGCTTTTTTAGAGGGTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACAccc                                                                                  <  2:268707/148‑1 (MQ=255)
                                                          atGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCttt                                                                               <  6:63466/148‑1 (MQ=255)
                                                              cGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCg                                                                           >  5:61810/1‑148 (MQ=255)
                                                               gCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGa                                                                          >  7:4794/1‑148 (MQ=255)
                                                                       ttttttAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAg                                                                   >  4:15255/1‑147 (MQ=255)
                                                                            tAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTcc                                                             <  3:15255/148‑1 (MQ=255)
                                                                            tAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTcc                                                             <  4:279747/148‑1 (MQ=255)
                                                                                 gTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAg                                                       <  8:73157/149‑1 (MQ=255)
                                                                                     gCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATg                                                    >  6:156179/1‑148 (MQ=255)
                                                                                     gCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATg                                                    >  8:271324/1‑148 (MQ=255)
                                                                                      cTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATg                                                    >  1:45481/1‑147 (MQ=255)
                                                                                        gACGGAGAGCGCCAGGCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAgg                                                <  8:121274/149‑1 (MQ=255)
                                                                                         aCGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAg                                                 <  8:4794/147‑1 (MQ=255)
                                                                                          cGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGc                                               <  5:156179/148‑1 (MQ=255)
                                                                                                    cAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGt                                    <  7:271324/149‑1 (MQ=255)
                                                                                                     aGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCGAGTg                                   <  7:206600/149‑1 (MQ=255)
                                                                                                       tCATTTGTCTCCAGCTTATCAGACACTACGTGATGTTTCGGTAGTATAGCGACACATGTAGTGCTTTTGCCTCAGCCGGACAAGGTCTAAGCTGAGTCCCTTTTTCTTAATTCAGGAGTCCGGGTAGATGAAGGCAGGTATCGAGTgg                                  <  2:8526/148‑1 (MQ=255)
                                                                                                       tCATTTGGCGCCAGCTTATCAGACACAACGAGATTATTCGGTACTAAACAGAAACATCTCGTGCTTTTCCCACAGCCGCAAAACGTCTAAGCTGACACCCTTTTCCGAAAATAAGGATTCCGGCAAGATGAAGGCACGTATCAAGttt                                  >  1:8526/1‑146 (MQ=255)
                                                                                                                    gCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGCAAt                    >  8:235114/1‑149 (MQ=255)
                                                                                                                         tCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGCAATGTTcc               >  7:268504/1‑149 (MQ=255)
                                                                                                                             acacTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGCAATGTTCCTCgg           <  2:45481/149‑1 (MQ=255)
                                                                                                                                 tACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGc                       <  4:196845/133‑1 (MQ=255)
                                                                                                                                 tACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGc                       >  3:196845/1‑133 (MQ=255)
                                                                                                                                 caCGAGATGTGTCGGTTTAGTACCGAAACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAATATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAg                         <  1:73747/130‑1 (MQ=38)
                                                                                                                                 caCGAGATGTGTCGGTTTAGTACCGAAACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAg                         >  2:69242/2‑131 (MQ=38)
                                                                                                                                 caCGAGATGTGTCGGTTTAGTACCGAAACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAg                         >  2:73747/2‑131 (MQ=38)
                                                                                                                                 caCGAGATGTGTCGGTTTAGTACCGAAACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAg                         <  1:69242/130‑1 (MQ=38)
                                                                                                                                   cGAGATGTGTCGGTTTAGTACCGAAACATCTCGTGCTTTTCCCACAGCCGCACAAGATCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACATATCCAGTGGGCCGGTGAGGCAATGTTCCTCGGCGAATc     >  5:222950/1‑149 (MQ=255)
                                                                                                                                      gaTGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGCAATGTTCCTCGGCGAATCggg  <  6:61810/149‑1 (MQ=255)
                                                                                                                                       aTGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGc                                >  3:112578/1‑118 (MQ=255)
                                                                                                                                       aTGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGc                                <  4:112578/118‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                               
CGATGTTCTTTATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGCAATGTTCCTCGGCGAATCGGG  >  NC_002947/513314‑513596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑