Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,438,832 G→T 5.1% T175K (ACG→AAG)  rluE ← 23S rRNA pseudouridine(2457) synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,438,8320GT 5.1% 257.4 / 3.4 78T175K (ACG→AAG) rluE23S rRNA pseudouridine(2457) synthase
Reads supporting (aligned to +/- strand):  ref base G (38/36);  new base T (2/2);  total (40/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.87e-01

TAAAACATTTCAGGGCCATTCGCGGGCCCTGACCTGAACTAAAGCCTGGCAGGGACCTCCCGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCGGCGGGTTACGCGGCCACAGCTCGGGTTCTTCCAGGCGCCGCGCCTCAGCCGGCAAGGT  >  NC_002947/5438685‑5438974
                                                                                                                                                   |                                                                                                                                              
taAAACATTTCAGGGCCATTCGCGGCCCCTGACCTGAAAAAAAACCTGGCAGGCACCACCCGCCAACAACCCAGCACCAGCCCATCCAGCACCCAGACACCAATGAGCACCCGCACAACGCCCAAGATCAGCAAACCAACAGATGCCg                                                                                                                                                >  8:196018/1‑148 (MQ=255)
   aaCATTTCAGGGCCATTCGCGGGCCCTGACCTGAACTAAAGCCTGGCAGGGACCTCCCGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCAt                                                                                                                                            >  7:68436/1‑149 (MQ=255)
   aaCATTTCAGGGCCATTCGCGGGCCCTGACCTGAACTAAAGCCTGGCAGGGACCTCCCGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCAt                                                                                                                                            >  6:79842/1‑149 (MQ=255)
      aTTTCAGGGCCATTCGCGGGCCCTGACCTGAACTAAAGCCTGGCAGGGACCTCCCGCCAGCACCCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATccg                                                                                                                                         >  3:47171/1‑149 (MQ=255)
             ggtcATTCGCGGGCCCTGACCTGAACTAAAGCCTGGCAGGGACCTCCCGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCAcc                                                                                                                                  >  3:12535/4‑149 (MQ=255)
             ggCCATTCGCGGGCCCTGACCTGAACTAAAGCCTGGCAGGGACCTCCCGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCAcc                                                                                                                                  >  7:296508/1‑149 (MQ=255)
               ccATTCGCGGGCCCTGACCTGAACTAAAGCCTGGCAGGGACCTCCCGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTg                                                                                                                                >  3:287619/1‑149 (MQ=255)
                cATTCGCGGGCCCTGACCTTAACTAAAGCCTGGCAGGGACCTCCCGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTgg                                                                                                                               <  7:196018/149‑1 (MQ=255)
                  ttCGCGGGCCCTGACCTGAACTAAAGCCTGGCAGGGACCTCCCGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCg                                                                                                                             >  4:46704/1‑149 (MQ=255)
                          cccTGACCTGAACTAAAGCCTGGCAGGGACCTCCCGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGc                                                                                                                              >  5:174638/1‑140 (MQ=255)
                          cccTGACCTGAACTAAAGCCTGGCAGGGACCTCCCGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGc                                                                                                                              <  6:174638/140‑1 (MQ=255)
                                cctgaACGAAAGCCTGGCAGGGACCTCCCGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTcgc                                                                                                               <  2:298492/149‑1 (MQ=255)
                                 ctgaAATAAAGACTGGCAGGGACCTCCCTCCAGCAACCATGCTACAGAAAATACAGAGAACATTCACCAATGCGCACGCGCACCAGGCGCAAGATCGAAAATCCCACTGATGCAGTAATCCGACGCACATCGCAGTTACACACTTc                                                                                                                 >  8:146861/1‑146 (MQ=255)
                                  tgaACTAAAGCCTGGCAGGGACCTCCCGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTcgcg                                                                                                              >  3:204791/1‑148 (MQ=255)
                                     aCTAAAGCCTGGCAGGTACCTCCCGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATc                                                                                                          <  5:106439/149‑1 (MQ=255)
                                     aCTAAAGCCTGGCAGGGACCTCCCGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATc                                                                                                          <  5:79842/149‑1 (MQ=255)
                                         aaGCCTGGCAGGGACCTCCCGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCa                                                                                                      <  1:288160/149‑1 (MQ=255)
                                            ccTGGCAGGGACCTCCCGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGtt                                                                                                   >  5:138253/1‑149 (MQ=255)
                                             cTGGCAGGGACCTCCCGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGtt                                                                                                   <  7:146861/148‑1 (MQ=255)
                                                      aCCTCCCGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCt                                                                                         <  4:12535/149‑1 (MQ=255)
                                                        cTCCCGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTgg                                                                                       >  7:261719/1‑149 (MQ=255)
                                                         tCCCGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTgg                                                                                                                               <  7:259800/108‑1 (MQ=255)
                                                         tCCCGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTgg                                                                                                                               >  8:259800/1‑108 (MQ=255)
                                                           ccGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCAcc                                                                                                                                  >  2:114853/1‑103 (MQ=255)
                                                           ccGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCAcc                                                                                                                                  <  1:114853/103‑1 (MQ=255)
                                                                aGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACg                                                                               <  4:218073/149‑1 (MQ=255)
                                                                  cAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGTATCACCAGTTCCAGCCAGCTGGTCGGCACGct                                                                             >  3:93756/1‑149 (MQ=255)
                                                                   aaCCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGctc                                                                            >  7:49043/1‑149 (MQ=255)
                                                                    aCCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCTTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCa                                                                                                      >  6:147329/1‑122 (MQ=255)
                                                                    aCCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCTTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCa                                                                                                      <  5:147329/122‑1 (MQ=255)
                                                                    aCCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCTTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCa                                                                                                      <  5:151925/122‑1 (MQ=255)
                                                                    aCCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCTTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCa                                                                                                      >  6:151925/1‑122 (MQ=255)
                                                                      ccTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTg                                                                         >  8:74923/1‑149 (MQ=255)
                                                                      ccTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGGTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTg                                                                         >  7:171590/1‑149 (MQ=255)
                                                                      ccTGCTCCAGCCCATCCAGCGCCCAGTCACAAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTg                                                                         >  7:286864/1‑149 (MQ=255)
                                                                            ccAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAg                                                                                                     >  5:212507/1‑115 (MQ=255)
                                                                            ccAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAg                                                                                                     <  6:212507/115‑1 (MQ=255)
                                                                            ccAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGt                                                                                      <  7:3856/130‑1 (MQ=255)
                                                                            ccAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGt                                                                                      >  8:3856/1‑130 (MQ=255)
                                                                             cAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTcgc                                                                                                               >  8:173830/1‑104 (MQ=255)
                                                                             cAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAAcc                                                                  >  4:93257/1‑149 (MQ=255)
                                                                             cAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAAcc                                                                  >  8:222904/1‑149 (MQ=255)
                                                                             cAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAAcc                                                                  >  3:133551/1‑149 (MQ=255)
                                                                              aGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTc                                                                                                  <  1:61046/116‑1 (MQ=255)
                                                                              aGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTc                                                                                                  >  2:61046/1‑116 (MQ=255)
                                                                                      cAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACcggcg                                                         <  8:68436/149‑1 (MQ=255)
                                                                                      cAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACcggc                                                          <  7:222904/148‑1 (MQ=255)
                                                                                       aGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGTATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACcggcgg                                                        <  4:93756/149‑1 (MQ=255)
                                                                                       aGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCa                                                                                 >  1:24623/1‑124 (MQ=255)
                                                                                       aGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCa                                                                                 <  2:24623/124‑1 (MQ=255)
                                                                                       aGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACcggcgg                                                        <  8:296508/149‑1 (MQ=255)
                                                                                       aGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACcggcgg                                                        <  7:90939/149‑1 (MQ=255)
                                                                                          gcCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCGGCGGGtt                                                     <  4:204791/149‑1 (MQ=255)
                                                                                          gcCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCGGCGGGtt                                                     <  6:138253/149‑1 (MQ=255)
                                                                                            ccAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTcca                                                                                                >  1:123682/1‑104 (MQ=255)
                                                                                            ccAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTcca                                                                                                <  2:123682/104‑1 (MQ=255)
                                                                                            ccAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCGGCGGGTTAc                                                   >  1:287180/1‑149 (MQ=255)
                                                                                                  aCCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCGGCGGGTTACGCGGc                                              <  4:47171/148‑1 (MQ=255)
                                                                                                   ccAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCGGCGGGTTACGCGGCca                                            <  7:276450/149‑1 (MQ=255)
                                                                                                    cAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCCCCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCGGCGGGTTACGCGGCca                                            >  2:40528/1‑148 (MQ=255)
                                                                                                    cAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCGGCGGGTTACGCGGCca                                            >  2:152317/1‑148 (MQ=255)
                                                                                                    cAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCGGCGGGTTACGCGGCca                                            <  1:152317/148‑1 (MQ=255)
                                                                                                          gcacgcgcacCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGc                                                                        >  4:190497/1‑114 (MQ=255)
                                                                                                            acgcgcacCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTa                                                               <  7:277816/121‑1 (MQ=255)
                                                                                                            acgcgcacCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTa                                                               >  8:277816/1‑121 (MQ=255)
                                                                                                            acgcgcacCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCGGCGGGTTACGCGGCCACAGCTCggg                                   <  7:74923/149‑1 (MQ=255)
                                                                                                            acgcgcacCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCAGCGGGTTACGCGGCCACAGCTCggg                                   <  5:8342/149‑1 (MQ=255)
                                                                                                               cgcacCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCGGCGGGTTACGCGGCCACAGCTCGGGttc                                <  4:287619/149‑1 (MQ=255)
                                                                                                               cgcacCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCGGCGGGTTACGCGGCCACAGCTCGGGttc                                <  8:171590/149‑1 (MQ=255)
                                                                                                                gcacCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCGGCGGGTTACGCGGCCACAGCTCGGGttct                               <  8:286864/149‑1 (MQ=255)
                                                                                                                 cacCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCGGCGGGTTACGCGGCCACAGCTCGGGttctt                              >  8:214532/1‑149 (MQ=255)
                                                                                                                    cAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCGGCGGGTTACGCGGCCACAGCTCGGGttcttc                             >  1:120891/1‑147 (MQ=255)
                                                                                                                    cAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCGGCGGGTTACGCGGCCACAGCTCGGGTTCTTCCa                           <  3:46704/149‑1 (MQ=255)
                                                                                                                      ggCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCGGCGGGTTACGCGGCCACAGCTCGGGTTCTTCCAgg                         <  2:287180/149‑1 (MQ=255)
                                                                                                                                         cACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCGGCGGGTTACGCGGCCACAGCTCGGGTTCTTCCAGGCGCCGCGCCTCAGCCGGc       >  6:319952/1‑148 (MQ=255)
                                                                                                                                            tgctgcCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTCCCGGCGGGTTACGCGGCCACAGCTCGGGTTCTTCCAGGCGCCGCGCCTCAGCCGGCAAgg   >  7:207696/1‑149 (MQ=255)
                                                                                                                                              ctgcCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCGGCGGGTTACGCGGCCACAGCTCGGGTTCTTCCAGGCGCCGCGCCTCAGCCGGCAAGGt  <  7:214532/148‑1 (MQ=255)
                                                                                                                                                 ccGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCGGCGGGTTACGCGGCCACAGCTCGGGTTCTTCCAGGCGCCGCGCCTCa             >  6:301360/1‑134 (MQ=255)
                                                                                                                                                 ccGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCGGCGGGTTACGCGGCCACAGCTCGGGTTCTTCCAGGCGCCGCGCCTCa             <  5:301360/134‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
TAAAACATTTCAGGGCCATTCGCGGGCCCTGACCTGAACTAAAGCCTGGCAGGGACCTCCCGCCAGCAACCCTGCTCCAGCCCATCCAGCGCCCAGTCACCAATGCGCACGCGCACCAGGCGCAAGGTCGGCAAGCCCACTGCTGCCGTCATCCGCCGCACCTGGCGGTTACGCCCTTCGCGAATCACCAGTTCCAGCCAGCTGGTCGGCACGCTCTTGCGAAACCGTACCGGCGGGTTACGCGGCCACAGCTCGGGTTCTTCCAGGCGCCGCGCCTCAGCCGGCAAGGT  >  NC_002947/5438685‑5438974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑