Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,457,903	G→A	6.6%	L545L (CTG→CTA)	leuS →	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,457,903	0	G	A	6.6%	177.9 / 2.6	60	L545L (CTG→CTA)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (26/30);  new base A (2/2);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

TGGACACCTTCGTCGAGTCGTCCTGGTACTTCGCCCGCTACGCCTCGCCAAACTACGACAAAGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTCGCCGAAACCTACTACCGCGTTGCCAGCAACGGCGGCAAGGACTGGTTCAACC  >  NC_002947/5457758‑5458051                                                                                                                                                    |                                                                                                                                                      tGGACACCTTCGTCGAGTCGTCCTGGTACTTCGCCCGCTACGCCTCGCCAAACTACGACAAAGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑Cctgctg                                                                                                                                                    >  3:218958/1‑149 (MQ=255)        cTTCGTCGAGTCGTCCTGGTACTTCGCCCGCTACGCCTCGCCAAACTACGACAAAGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTAcgcgc                                                                                                                                             >  1:297906/1‑149 (MQ=255)        cTTCGTCGAGTCGTCCTGGTACTTCGCCCGCTACGCCTCGCCAAACTACGACAAAGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTACTGTAcgcgc                                                                                                                                             >  6:29286/1‑149 (MQ=255)               gAGTCGTCCTGGTACTTCGCCCGCTACGCCTCGCCAAACTACGACAAAGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGcttctt                                                                                                                                      >  7:96627/1‑149 (MQ=255)                aGTCGTCCTGGTACTTCGCCCGCTACGCCTCGCCAAACTACGACAAAGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCttcttc                                                                                                                                     <  8:64587/149‑1 (MQ=255)                          gTACTTCGCCCGCTACGCCTCGCCAAACTACGACAAAGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGa                                                                                                                           <  5:248820/149‑1 (MQ=255)                          gTACTTCGCCCGCTACGCCTCGCCAAACTACGACAAAGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGa                                                                                                                           >  2:24464/1‑149 (MQ=255)                                cGCCCGCTACGCCTCGCCAAACTACGACAAAGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGt                                                                                                                      <  2:297906/148‑1 (MQ=255)                                   ccGCTACGCCTCGCCAAACTACGACAAAGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTACTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACg                                                                                                                  <  5:29286/149‑1 (MQ=255)                                      cTACGCCTCGCCAAACTACGACAAAGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAg                                                                                                               <  4:218958/149‑1 (MQ=255)                                         cGCCTCGCCAAACTACGACAAAGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGc                                                                                                             <  1:24464/148‑1 (MQ=255)                                          gCCTCGCCAAACTACGACAAAGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCt                                                                                                           >  2:209134/1‑149 (MQ=255)                                           ccTCGCCAAACTACGACAAAGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTg                                                                                                          >  3:2245/1‑149 (MQ=255)                                               gacAAACTACGACAAAGGCCTTGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCTGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTc                                                                                                       <  5:310551/146‑1 (MQ=255)                                               gCCAAACTACGACAAGGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTg                                                                                                          >  1:39535/1‑145 (MQ=255)                                               gCCAAACTACGACAAGGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTg                                                                                                          <  2:39535/145‑1 (MQ=255)                                                 cAAACTACGACAAAGGCCTGGTCGACCCCAAATCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCATCCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGc                                                                                                                        <  1:207456/130‑1 (MQ=255)                                                 cAAACTACGACAAAGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGc                                                                                                                        >  2:207456/1‑129 (MQ=255)                                                 cAAACTACGACAAAGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCAcc                                                                                                    >  2:91585/1‑149 (MQ=255)                                                   aaCTACGACAAAGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTc                                                                                                  >  4:135620/1‑149 (MQ=255)                                                      tACGACAAAGGCCTGGTCGACCCCAAAGCCGCCAGACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCg                                                                                                 >  1:202953/1‑148 (MQ=255)                                                      tACGACAAAGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGaa                                                                                               >  4:248000/1‑149 (MQ=255)                                                         gACAAAGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAAc                                                                                              >  5:243407/1‑147 (MQ=255)                                                           cAAAGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGc                                                                                          >  3:109169/1‑149 (MQ=255)                                                                gCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTc                                                                                    <  4:2245/149‑1 (MQ=255)                                                                  cTGGTCGACACCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTc                                                                                    >  5:95494/1‑147 (MQ=255)                                                                         aCCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACctg                                                                           <  4:266285/149‑1 (MQ=255)                                                                            ccAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTc                                                                                    <  4:135245/137‑1 (MQ=255)                                                                            ccAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTc                                                                                    >  3:135245/1‑137 (MQ=255)                                                                             cAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCt                                                                                                   >  3:286253/1‑122 (MQ=255)                                                                             cAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCt                                                                                                   <  4:286253/122‑1 (MQ=255)                                                                             cAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGa                                                                       <  3:135620/149‑1 (MQ=255)                                                                                   cgccA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAgg                                                                 >  5:309319/1‑149 (MQ=255)                                                                                    gccA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGAGCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAggg                                                                <  2:228381/149‑1 (MQ=255)                                                                                      cA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGAccc                                                                    <  6:126875/143‑1 (MQ=255)                                                                                      cA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGAccc                                                                    >  5:126875/1‑143 (MQ=255)                                                                                          ccACTGGCTGCCGGTAGATCATTACATCGGCGGTATCGAACACGCGATACTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATTCGTGACGAAGTCCTGGTCACCTCGAACGAGC‑AGTTCAAGAACCTGCTGACCCAGGGCAttg                                                           <  6:158810/149‑3 (MQ=255)                                                                                           cACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTc                                                                                                       <  6:105769/105‑1 (MQ=255)                                                                                           cACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTc                                                                                                       >  5:105769/1‑105 (MQ=255)                                                                                            aCTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCAtggtg                                                         <  7:266799/149‑1 (MQ=255)                                                                                                gCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTc                                                      <  8:96627/148‑1 (MQ=255)                                                                                                      ggTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTCGCCGaaa                                               <  6:309319/149‑1 (MQ=255)                                                                                                       gTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAgg                                                                 >  5:21597/1‑130 (MQ=255)                                                                                                       gTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAgg                                                                 <  6:21597/130‑1 (MQ=255)                                                                                                       gTACATCACTACCTCGGCGGTAGCGAACACGCGATCCAGCA‑CCTGCTGGACGCGCCCTTCTTCCACAAGCTGCTGCGGGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTCGCCGAAAc                                              <  4:230531/149‑1 (MQ=255)                                                                                                          gATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTCGCCGAAACcta                                           <  1:209134/149‑1 (MQ=255)                                                                                                                  cATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTCGCCGAAACCTACTACcgcg                                   <  6:95494/149‑1 (MQ=255)                                                                                                                  cATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTCGCCGAAACCTACTACcgc                                    <  4:109169/148‑1 (MQ=255)                                                                                                                   aTCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCAcc                                                                                                    <  8:49237/84‑1 (MQ=255)                                                                                                                   aTCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCAcc                                                                                                    >  7:49237/1‑84 (MQ=255)                                                                                                                      ggcggTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTCGCCGAAACCTACTACCGCGTTGc                               >  3:226491/1‑149 (MQ=255)                                                                                                                       gcggTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTCGCCGAAACCTACTACCGCGTTGc                               >  1:56125/1‑148 (MQ=255)                                                                                                                               gAACACGCGATCCTGCA‑CCTACTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTCGCCGAAACCTACTACCGCGTTGCCAGCAAcgg                      >  3:116291/1‑149 (MQ=255)                                                                                                                                aaCACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTCGCCGAAACCTACTACCGCGTTGCCAGCAAcggc                     >  2:81366/1‑149 (MQ=255)                                                                                                                                 acacGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTCGCCGAAACCTACTACCGCGTTGCCAGCAAcggcg                    <  2:202953/149‑1 (MQ=255)                                                                                                                                  cacGCGATCCTGCA‑CCTACTGTACGCGCGCTTCTTCCACAAGCTGATGCGTTACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTCGCCGAAACCTACTACCGCGTTGCCAGCAAcggcgg                   <  4:116291/149‑1 (MQ=255)                                                                                                                                    cgcgATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTCGCCGAAACctac                                          >  7:230644/1‑124 (MQ=255)                                                                                                                                    cgcgATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTCGCCGAAACctac                                          <  8:230644/124‑1 (MQ=255)                                                                                                                                      cgATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTCGCCGAAACCTACTACCGCGTTGCCAGCAACGGCGGCAAg               <  3:248000/149‑1 (MQ=255)                                                                                                                                            tGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTCGCCGAAACCTACTACCGCGTTGCCAGCAACGGCGGCAAGGACTgg         >  3:196543/1‑149 (MQ=255)                                                                                                                                              cA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTCGCCGAAACCTACTACCGCGTTGCCAGCAACGGCGGCAAGGACTGGtt       <  4:226491/149‑1 (MQ=255)                                                                                                                                                  ctgctgTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTCGCCGAAACCTACTACCGCGTTGCCAGCAACGGCGGCAAGGACTGGTTCaa    >  7:253844/1‑149 (MQ=255)                                                                                                                                                   tgctgTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGCAAGTTCAAGAACATGATGACCCAGGGCTGGGTGTTCGCCGAACCCTACTACCGCGTTGCCAGCAACGCCGGCAAGGACTGGTTCaa    >  1:252617/1‑149 (MQ=255)                                                                                                                                                    gctgTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTCGCCGAAACCTACTACCGCGTTGCCAGCAACGGCGGCAAGGACTGGTTCAAcc  >  5:58958/1‑149 (MQ=255)                                                                                                                                                    gctgTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTCGCCGAAACCTACTACCGCGTTGCCAGCAACGGCGGCAAGGACTGGTTCAAcc  >  1:69530/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                      TGGACACCTTCGTCGAGTCGTCCTGGTACTTCGCCCGCTACGCCTCGCCAAACTACGACAAAGGCCTGGTCGACCCCAAAGCCGCCA‑ACCACTGGCTGCCGGTAGATCAGTACATCGGCGGTATCGAACACGCGATCCTGCA‑CCTGCTGTACGCGCGCTTCTTCCACAAGCTGATGCGTGACGAAGGCCTGGTCACCTCGAACGAGC‑CGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTCGCCGAAACCTACTACCGCGTTGCCAGCAACGGCGGCAAGGACTGGTTCAACC  >  NC_002947/5457758‑5458051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑