Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,458,541	T→C	6.8%	L758P (CTG→CCG)	leuS →	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,458,541	0	T	C	6.8%	173.6 / 2.2	59	L758P (CTG→CCG)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (28/27);  new base C (2/2);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ATCCGCCGCGCCATTCACGCTGCCATCAAGCAGGCCAGCACCGACGTGGGCCAGTTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTGGGCCACGCAGGCTCGGTGATCGATGCCGCCTGGCCAAGCGTCGACGAACAGGCGCTGGTGCAG  >  NC_002947/5458393‑5458683                                                                                                                                                     |                                                                                                                                               aTCCGCCGCGCCATTCACGCTGCCATCAAGCAGGCCAGCACCGACGTGGGCCAGTTCCACAAGTTCAATACCGCCATCGACCAGGTGATGACCGTGATTAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCctgct                                                                                                                                                <  1:235326/149‑1 (MQ=255)             aTTCACGCTGCCATCAAGCAGGCCAGCACCGACGTGGGCCAGGTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCt                                                                                                                                    <  8:240334/149‑1 (MQ=255)              ttaaCGCTGCCATCAAGCAGGCCAGCACCGACGTGGGCCAGTTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCtgctgc                                                                                                                                              >  3:13903/4‑138 (MQ=255)              ttCACGCTGCCATCAAGCAGGCCAGCACCGACGTGGGCCAGTTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCTCCCtgctgc                                                                                                                                              <  4:13903/138‑1 (MQ=255)                 aCGCTGCCATCAAGCAGGCCAGCACCGACGTGGGCCAGTTCCACAAGTTCAATACCGCCATCGCCCAGGTGCCGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTgg                                                                                                                                  >  5:61941/1‑147 (MQ=255)                    cTGCCATCAAGCAGGCCAGCACCGACGTGGGCCAGTTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGcc                                                                                                                             <  4:270333/149‑1 (MQ=255)                       ccATCAAGCAGGCCAGCACCGACGTGGGCCAGTTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTgg                                                                                                                                  <  3:67600/141‑1 (MQ=255)                       ccATCAAGCAGGCCAGCACCGACGTGGGCCAGTTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTgg                                                                                                                                  >  4:67600/1‑141 (MQ=255)                         aTCAAGCAGGCCAGCACCGACGTGGGCCAGTTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCt                                                                                                                                    >  5:289522/1‑137 (MQ=255)                         aTCAAGCAGGCCAGCACCGACGTGGGCCAGTTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCt                                                                                                                                    <  6:289522/137‑1 (MQ=255)                         aTCAAGCAGGCCAGCACCGACGTGGGCCAGTTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCAc                                                                                                                        >  6:37969/1‑149 (MQ=255)                         aTCAAGCAGGCCAGCACCGACGTGGGCCAGTTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCAc                                                                                                                        >  2:314524/1‑149 (MQ=255)                             aGCAGGCCAGCACCGACGTGGGCCAGTTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCctg                                                                                                                    >  1:127977/1‑149 (MQ=255)                                     aGCACCGACGTGGGCCAGTTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGgc                                                                                                            >  8:101890/1‑149 (MQ=255)                                          cGACGTGGGCCAGTTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGa                                                                                                       >  8:126927/1‑149 (MQ=255)                                          cGACGTGGGCCAGTTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGa                                                                                                       <  1:314524/149‑1 (MQ=255)                                                gggCCAGTTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCctgc                                                                                                                   >  7:8246/1‑131 (MQ=255)                                                 ggCCAGTTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTc                                                                                                 <  3:24474/148‑1 (MQ=255)                                                   ccAGTTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCg                                                                                               >  7:260559/1‑148 (MQ=255)                                                   ccAGTTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCCGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTcctc                                                                                              >  8:259840/1‑147 (MQ=255)                                                   ccAGTTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCCGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCg                                                                                               >  8:264250/1‑148 (MQ=255)                                                      gTTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGaa                                                                                                                                >  4:155614/1‑112 (MQ=255)                                                      gTTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGaa                                                                                                                                <  3:155614/112‑1 (MQ=255)                                                       ttCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACAt                                                                                          <  7:11557/149‑1 (MQ=255)                                                          cacaAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACAtctc                                                                                       >  1:62157/1‑149 (MQ=255)                                                          cacaAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACAtctc                                                                                       >  6:154341/1‑149 (MQ=255)                                                           acaAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACAtctct                                                                                      >  3:36641/1‑149 (MQ=255)                                                                ttCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCAcgc                                                                                 >  7:116667/1‑149 (MQ=255)                                                                  cAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACgcgc                                                                               >  2:2393/1‑149 (MQ=255)                                                                   aaTACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGcc                                                                                                                             <  1:249960/102‑1 (MQ=255)                                                                   aaTACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGcc                                                                                                                             >  2:249960/1‑102 (MQ=255)                                                                     tACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCtgt                                                                            <  7:101890/149‑1 (MQ=255)                                                                     tACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCtgt                                                                            <  5:37969/149‑1 (MQ=255)                                                                        cGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGgc                                                                         <  2:127977/149‑1 (MQ=255)                                                                            aTCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGgcagca                                                                     >  1:167414/1‑149 (MQ=255)                                                                                cccAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTg                                                                 >  6:267783/1‑149 (MQ=255)                                                                                cccAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTg                                                                 <  4:302174/149‑1 (MQ=255)                                                                                 ccAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCCGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTgg                                                                <  7:259840/149‑1 (MQ=255)                                                                                 ccAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCCGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTgg                                                                <  7:264250/149‑1 (MQ=255)                                                                                        atgaCCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTGGGCCACGc                                                         <  8:260559/149‑1 (MQ=255)                                                                                              gTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTGGGCCACGCAGGCTc                                                   >  8:152803/1‑149 (MQ=255)                                                                                                 atgaACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTGGGCCACGCAGGCTCgg                                                 <  8:116667/148‑1 (MQ=255)                                                                                                   gaACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTGGGCCACGCAGGCTCGGTGa                                              >  6:72147/1‑149 (MQ=255)                                                                                                      cGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTgg                                                                <  4:210031/128‑1 (MQ=255)                                                                                                      cGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTgg                                                                >  3:210031/1‑128 (MQ=255)                                                                                                           tGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTGGGCCACGCAGGCTCGGTGATCGATgcc                                      >  2:300144/1‑149 (MQ=255)                                                                                                                     cccGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTGGGCCACGCAGGCTCGGTGATCGATGCCGCCTGGCCaa                            <  1:2393/149‑1 (MQ=255)                                                                                                                      ccGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTGGGCCACGCAGGCTCGGTGATCGATGCCGCCTGGCCAAg                           >  4:151654/1‑149 (MQ=255)                                                                                                                        gCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTGGGCCACGCAGGCTCGGTGAt                                             <  7:296850/129‑1 (MQ=255)                                                                                                                           aGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTGGGCCACGCAGGCTCGGTGATCGATGCCGCCTGGCCAAGCGTc                       >  5:37898/1‑148 (MQ=255)                                                                                                                            gCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTGGGCCACGCAGGCTCGGTGATCGATGCCGCCTGGCCAAGCGTcga                     <  5:72147/149‑1 (MQ=255)                                                                                                                            gCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTGGGCCACGCAGGCTCGGTGATCGATGCCGCCTGGCCAAGCGTcga                     <  5:185533/149‑1 (MQ=255)                                                                                                                              cACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTGGGCCACGCAGGCTCGGTGATCGATGCCGCCTGGCCAAGCGTcgacg                   <  7:126927/149‑1 (MQ=255)                                                                                                                              cACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTGGGCCACGCAGGCTCGGTGATCGACGCCGCCTGGCCAAGCGTcgac                    >  4:38070/1‑148 (MQ=255)                                                                                                                               aCCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTATGGCAGCACCTGGGCCACGCAGGCTCGGTGATCGATGCCGCCTGGCCAAGCGTcgacga                  <  4:231214/149‑1 (MQ=255)                                                                                                                                 cGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTGGGCCACGCAGGCTCGGTGATCGATGCCGCCTGGCCAAGCGTCGACGAAc                <  4:36641/149‑1 (MQ=255)                                                                                                                                   aaCAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTGGGCCACGCAGGCTCGGTGATCGATGCCGCCTGGCCAAGCGTCGACGAACAg              <  2:62157/149‑1 (MQ=255)                                                                                                                                          ccGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTGGGCCACGCAGGCTCGGTGATCGATGCCGCCTGGCCAAGCGTCGACGAACAGGCGCTgg       >  7:226272/1‑149 (MQ=255)                                                                                                                                           cgcgCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACAtctctc                                                                                     <  6:48804/70‑1 (MQ=255)                                                                                                                                           cgcgCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACAtctctc                                                                                     >  5:48804/1‑70 (MQ=255)                                                                                                                                           cgcgCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTGGGCCACGCAGGCTCGGTGATCGATGCCGCCTGGCCAAGCGTCGACGAACAGGCGCTGGt      <  2:167414/149‑1 (MQ=255)                                                                                                                                               cccTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTGGGCCACGCAGGCTCGGTGATCGATGCCGCCTGGCCAAGCGTCGACGAACAGGCGCTGGTGCAg  <  7:152803/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                               ATCCGCCGCGCCATTCACGCTGCCATCAAGCAGGCCAGCACCGACGTGGGCCAGTTCCACAAGTTCAATACCGCCATCGCCCAGGTGATGACCGTGATGAACGTGCTGGAAAAGGCCCCGCAAGCCACCGAACAGGACCGCGCCCTGCTGCAGGAAGGCCTGGAAGCCGTCACCCTGCTGCTGGCGCCGATCACTCCGCACATCTCTCACGCGCTGTGGCAGCACCTGGGCCACGCAGGCTCGGTGATCGATGCCGCCTGGCCAAGCGTCGACGAACAGGCGCTGGTGCAG  >  NC_002947/5458393‑5458683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑