Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,459,232 A→T 9.3% D99V (GAC→GTC)  PP_4795 → lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,459,2320AT 9.3% 122.0 / 3.1 43D99V (GAC→GTC) PP_4795lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (19/20);  new base T (2/2);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCGTGAAGTGCTGGAACGTAGCGGCGTCAACGTGCACGCCGGCGCACCGTATCGCCTGGTGCTGACCAACGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATCACCGGCTCCGAGCAGGAAGCCAAACGCGCCCGCGAAGAAATGCGCCGCGACCTGGT  >  NC_002947/5459084‑5459376
                                                                                                                                                    |                                                                                                                                                
cTGCGTGAAGTGCTGGAACGTAGCGGCGTCAACGTGCACGCCGGCGCACCGTATCGCCTGGTGCTGACCAACGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGa                                                                                                                                                  >  7:5674/1‑149 (MQ=255)
cTGCGTGAAGTGCTGGAACGTAGCGGCGTCAACGTGCACGCCGGCGCACCGTATCGCCTGGTGCTGACCAACGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGa                                                                                                                                                  <  5:188123/149‑1 (MQ=255)
        aGTGCTGGAACGTAGCGGCGTCAACGTGCACGCCGGCGCACCGTATCGCCTGGTGCTGACCAACGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGa                                                                                                                                                  <  8:170356/141‑1 (MQ=255)
        aGTGCTGGAACGTAGCGGCGTCAACGTGCACGCCGGCGCACCGTATCGCCTGGTGCTGACCAACGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGa                                                                                                                                                  >  7:170356/1‑141 (MQ=255)
             tGGAACGTAGCGGCGTCAACGTGCACGCCGGCGCACCGTATCGCCTGGTGCTGACCAACGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTg                                                                                                                                     >  1:198933/1‑149 (MQ=255)
                 aCGTAGCGGCGTCAACGTGCACGCCGGCGCACCGTATCGCCTGGTGCTGACCAACGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGcc                                                                                                                                 >  3:86321/1‑149 (MQ=255)
                    tAGCGGCGTCAACGTGCACGCCGGCGCACCGTATCGCCTGGTGCTGACCAACGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTg                                                                                                                               >  5:165959/1‑148 (MQ=255)
                         gcgTCAACGTGCACGCCGGCGCACCGTATCGCCTGGTGCTGACCAACGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGG‑CTGAACAAcc                                                                                                                        <  4:242229/149‑1 (MQ=255)
                            tCAACGTGCACGCCGGCGCACCGTATCGCCTGGTGCTGACCAACGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTg                                                                                                                      <  1:62454/149‑1 (MQ=255)
                            tCAACGTGCACGCCGGCGCACCGTATCGCCTGGTGCTGACCAACGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTg                                                                                                                      >  3:37376/1‑149 (MQ=255)
                              aaCGTGCACGCCGGCGCACCGTATCGCCTGGTGCTGACCAACGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTgg                                                                                                                     <  7:185281/148‑1 (MQ=255)
                                   gCACGCCGGCGCACCGTATCGCCTGGTGCTGACCAACGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCtga                                                                                                               >  1:159333/1‑149 (MQ=255)
                                       gCCGGCGCACCGTATCGCCTGGTGCTGACCAACGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAg                                                                                                           <  2:198933/149‑1 (MQ=255)
                                             gcACCGTATCGCCTGGTGCTGACCAACGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACaa                                                                                                     <  4:37376/149‑1 (MQ=255)
                                                   tATCGCCTGGTGCTGACCAACGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGa                                                                                               <  8:5674/149‑1 (MQ=255)
                                                               cTGACCAACGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGt                                                                                   <  8:89098/149‑1 (MQ=255)
                                                                   ccAACGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTAc                                                                               <  4:159845/149‑1 (MQ=255)
                                                                    cAACGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGt                                                                                            >  6:19365/1‑135 (MQ=255)
                                                                    cAACGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGt                                                                                            <  5:19365/135‑1 (MQ=255)
                                                                     aaCGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTAcgt                                                                             >  3:91031/1‑149 (MQ=255)
                                                                      aCGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTAcgtg                                                                            >  6:67/1‑149 (MQ=255)
                                                                       cGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTAcgtgc                                                                           >  1:241743/1‑149 (MQ=255)
                                                                         aaCAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGc                                                                                        <  4:124687/134‑1 (MQ=255)
                                                                              gagcgcgagcgcTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACgg                                                                    >  1:197272/1‑149 (MQ=255)
                                                                                            ggCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCg                                                                <  7:182933/139‑1 (MQ=255)
                                                                                            ggCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCg                                                                >  8:182933/1‑139 (MQ=255)
                                                                                              cGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATCACCGGc                                                    <  6:165959/149‑1 (MQ=255)
                                                                                                 ccTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATc                                                          >  7:299139/1‑140 (MQ=255)
                                                                                                 ccTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATc                                                          <  8:299139/140‑1 (MQ=255)
                                                                                                  cTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATCACCGGCTcc                                                 >  1:92558/1‑148 (MQ=255)
                                                                                                  cTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATCACCGGCTcc                                                 >  7:200545/1‑148 (MQ=255)
                                                                                                   tACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATCACCGGCTcc                                                 >  5:188154/1‑147 (MQ=255)
                                                                                                    acaacaGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGTCTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATCACCGGCTCCGAg                                              >  4:196201/1‑149 (MQ=255)
                                                                                                    acaacaGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGTCTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATCACCGGCTCCGAg                                              >  4:200061/1‑149 (MQ=255)
                                                                                                     caacaGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATCACCGGCTCCGAGc                                             <  2:159333/149‑1 (MQ=255)
                                                                                                      aacaGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATCACCGGCTCCGAGCa                                            <  8:200545/149‑1 (MQ=255)
                                                                                                             gCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATCACCGGCTCCGAGCAGGAAGcc                                     <  2:241743/149‑1 (MQ=255)
                                                                                                              cAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATCACCGGCTCCGAGCAGGAAGCCa                                    <  4:91031/149‑1 (MQ=255)
                                                                                                                 ccGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATCACCGGCTCCGAGCAGGAAGCCAAAc                                 <  5:67/149‑1 (MQ=255)
                                                                                                                          cgAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATCACCGGCTCCGAGCAGGAAGCCAAACGCGCCcgc                         >  7:213033/1‑148 (MQ=255)
                                                                                                                                cGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATCACCGGCTCCGAGCAGGaa                                        >  7:191935/1‑127 (MQ=255)
                                                                                                                                cGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATCACCGGCTCCGAGCAGGaa                                        <  8:191935/127‑1 (MQ=255)
                                                                                                                                 gAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATCACCGGCTCCGAGCAGGAAGCCAAACGCGCCCGCGAAGAAAt                 >  2:175346/1‑149 (MQ=255)
                                                                                                                                       accaccAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATCACCGGCTCCGAGCAGGAAGCCAAACGCGCCCGCGAAGAAATGcgccg           <  6:188154/149‑1 (MQ=255)
                                                                                                                                             aaGCTGGTCTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATCACCGGCTCCGAGCAGGAAGCCAAACGCGCCCGCGAAGAAATGCGCCGCGACCt     <  3:196201/149‑1 (MQ=255)
                                                                                                                                             aaGCTGGTCTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATCACCGGCTCCGAGCAGGAAGCCAAACGCGCCCGCGAAGAAATGCGCCGCGACCt     <  3:200061/149‑1 (MQ=255)
                                                                                                                                                 tGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATCACCGGCTCCGAGCAGGAAGCCAAACGCGCCCGCGAAGAAATGCGCCGCGACCTGGt  >  7:4750/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CTGCGTGAAGTGCTGGAACGTAGCGGCGTCAACGTGCACGCCGGCGCACCGTATCGCCTGGTGCTGACCAACGAACAGGAGCGCGAGCGCTCGGCGACCTACAACAGCGGCAACCGTACCGCCGAGTACGAGCTGACCACCAAGCTGGACTACAGCATCCTGGGCCTGAACAACCTGGAGCTGATGAGCGACAAGCTGGAGGTACGCAAGGTCTACGTGCGTGACGGCTCGAACATCACCGGCTCCGAGCAGGAAGCCAAACGCGCCCGCGAAGAAATGCGCCGCGACCTGGT  >  NC_002947/5459084‑5459376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑