Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,464,858 A→T 7.7% L87Q (CTG→CAG)  lipB ← octanoyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,464,8580AT 7.7% 151.2 / 2.7 52L87Q (CTG→CAG) lipBoctanoyltransferase
Reads supporting (aligned to +/- strand):  ref base A (22/26);  new base T (2/2);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

TCCGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGAT  >  NC_002947/5464719‑5465006
                                                                                                                                            |                                                                                                                                                    
tCCGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGcca                                                                                                                                             >  3:309799/1‑149 (MQ=255)
  cGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGccac                                                                                                                                            <  4:309799/148‑1 (MQ=255)
    tCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAg                                                                                                                                         >  4:1041/1‑149 (MQ=255)
    tCGACATAGACACCCGGGGCATAGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACCGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGccacca                                                                                                                                          <  1:233747/149‑1 (MQ=255)
         aTAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCTGGTGGCCTACCtgctgct                                                                                                                                    >  5:150141/1‑148 (MQ=255)
         aTAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCTGGTGGCCTACCtgctgct                                                                                                                                    <  6:150141/149‑2 (MQ=255)
          tAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGccc                                                                                                                                   >  3:208447/1‑149 (MQ=255)
            gACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCTGGTGGCCTACCTGCt                                                                                                                                       <  2:93970/143‑1 (MQ=255)
            gACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGACAGCTAGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCTGGTGGCCTACCTGCt                                                                                                                                       >  1:93970/1‑143 (MQ=255)
              cacCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCACCAGCAGGTAGGCCACCAGCTGCCCgggg                                                                                                                               <  1:176932/149‑1 (MQ=255)
               acCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGc                                                                                                                              >  4:240060/1‑149 (MQ=255)
                  cGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAACGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCAt                                                                                                                           <  7:222292/149‑1 (MQ=255)
                     ggCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGt                                                                                                                        <  6:298237/149‑1 (MQ=255)
                       cATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAg                                                                                                                      <  2:139645/149‑1 (MQ=255)
                        aTCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAgg                                                                                                                     >  2:167614/1‑149 (MQ=255)
                            tgCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGAc                                                                                                                 <  3:142604/148‑1 (MQ=255)
                              cTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCt                                                                                                               >  3:62785/1‑149 (MQ=255)
                               ttGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCCGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTg                                                                                                              >  8:162137/1‑149 (MQ=255)
                                  gCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGgc                                                                                                            <  3:1041/148‑1 (MQ=255)
                                        gCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCcgcg                                                                                                     >  5:230770/1‑149 (MQ=255)
                                        gCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCcgcg                                                                                                     >  4:38066/1‑149 (MQ=255)
                                          cTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGcggt                                                                                                   >  3:295542/1‑149 (MQ=255)
                                             aaCAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGcggtcg                                                                                                 <  6:212372/148‑1 (MQ=255)
                                             aaCAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGcggtcg                                                                                                 <  7:162137/148‑1 (MQ=255)
                                             aaCAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGcggtc                                                                                                  <  3:38066/147‑1 (MQ=255)
                                              aCAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGcggtcggt                                                                                               <  1:167614/149‑1 (MQ=255)
                                                    tAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGcac                                                                                         <  8:53673/149‑1 (MQ=255)
                                                      gCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGcacca                                                                                       <  5:157564/149‑1 (MQ=255)
                                                         ggCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCg                                                                                    >  6:80755/1‑149 (MQ=255)
                                                             aGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAAt                                                                                <  4:62785/149‑1 (MQ=255)
                                                                   tCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGcc                                                                          >  3:38515/1‑149 (MQ=255)
                                                                   tCGATGAGGGCCAGCTCGATACGGCTGACAAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGACCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCTCCACCGGAATGTCgac                                                                          >  3:42512/1‑147 (MQ=255)
                                                                     gATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGcggtcg                                                                                                 <  4:220749/124‑1 (MQ=255)
                                                                     gATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGcggtcg                                                                                                 >  3:220749/1‑124 (MQ=255)
                                                                      aTGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGcca                                                                                                                                             >  8:2577/1‑79 (MQ=255)
                                                                      aTGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGcca                                                                                                                                             <  7:2577/79‑1 (MQ=255)
                                                                          gggCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCAc                                                                    >  3:14450/1‑148 (MQ=255)
                                                                               aGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACcagcag                                                              <  3:240060/149‑1 (MQ=255)
                                                                                gCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAgg                                                             >  1:221902/1‑149 (MQ=255)
                                                                                  tCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCgg                                                                       <  1:13847/137‑1 (MQ=255)
                                                                                  tCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCgg                                                                       >  2:13847/1‑137 (MQ=255)
                                                                                  tCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTg                                                           >  1:316462/1‑149 (MQ=255)
                                                                                      tACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCTGCTGGTGccgg                                                       <  5:80755/149‑4 (MQ=255)
                                                                                        cGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCTGCTGGTGccgggc                                                     <  4:208447/149‑6 (MQ=255)
                                                                                        cGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGc                                                     <  2:316462/149‑1 (MQ=255)
                                                                                               ccAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCg                                              >  2:62569/1‑149 (MQ=255)
                                                                                                           acgcCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTg                                   >  1:280909/1‑149 (MQ=255)
                                                                                                            cgcCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTgg                                  <  2:221902/149‑1 (MQ=255)
                                                                                                             gcCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTggg                                 >  1:182435/1‑149 (MQ=255)
                                                                                                              ccAACGCCCAGCCCGCGCACATCCAGCCGCAGGTAGTCCACCACCTGCCCGGGGCCATGGTAGGCGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCACGTGCTCGCCCTTGCCGGCCTGCCCCTGGGt                                <  4:42512/149‑1 (MQ=255)
                                                                                                              ccAAAGCCCCGCCGCCGCACATCCCGCAGCAGGTAGGCCACCCGCAGCCCGGGGCCATGGGAGGTGACCTGGCCGCCGCGGTCGGTCTGCCCCACCGGAATGTCGCCCGGCACCAGCAGGTGCGCGGCCTTGCCGGCCTGCCCCTGGGt                                <  4:38515/149‑1 (MQ=255)
                                                                                                                          cGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAgg                    <  2:182435/149‑1 (MQ=255)
                                                                                                                                       gcagcagGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGAccagc       >  8:116872/1‑149 (MQ=255)
                                                                                                                                          gcagGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGAccagc       >  8:22483/1‑146 (MQ=255)
                                                                                                                                            agGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGAt  <  4:14450/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                    
TCCGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGAT  >  NC_002947/5464719‑5465006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑