Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,464,864	G→C	7.1%	A85G (GCC→GGC)	lipB ←	octanoyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,464,864	0	G	C	7.1%	175.8 / 3.3	56	A85G (GCC→GGC)	lipB	octanoyltransferase
Reads supporting (aligned to +/- strand):  ref base G (24/27);  new base C (2/2);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

TCCGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGATT  >  NC_002947/5464719‑5465007                                                                                                                                                   |                                                                                                                                                tCCGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGcca                                                                                                                                              >  3:309799/1‑149 (MQ=255)   cGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGccac                                                                                                                                             <  4:309799/148‑1 (MQ=255)     tCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAg                                                                                                                                          >  4:1041/1‑149 (MQ=255)     tCGACATAGACACCCGGGGCATAGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACCGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGccacca                                                                                                                                           <  1:233747/149‑1 (MQ=255)          aTAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCTGGTGGCCTACCtgctgct                                                                                                                                     <  6:150141/149‑2 (MQ=255)          aTAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCTGGTGGCCTACCtgctgct                                                                                                                                     >  5:150141/1‑148 (MQ=255)           tAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGccc                                                                                                                                    >  3:208447/1‑149 (MQ=255)             gACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCTGGTGGCCTACCTGCt                                                                                                                                        <  2:93970/143‑1 (MQ=255)             gACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGACAGCTAGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCTGGTGGCCTACCTGCt                                                                                                                                        >  1:93970/1‑143 (MQ=255)               cacCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCACCAGCAGGTAGGCCACCAGCTGCCCgggg                                                                                                                                <  1:176932/149‑1 (MQ=255)                acCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGc                                                                                                                               >  4:240060/1‑149 (MQ=255)                   cGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAACGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCAt                                                                                                                            <  7:222292/149‑1 (MQ=255)                      ggCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGt                                                                                                                         <  6:298237/149‑1 (MQ=255)                        cATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAg                                                                                                                       <  2:139645/149‑1 (MQ=255)                         aTCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAgg                                                                                                                      >  2:167614/1‑149 (MQ=255)                             tgCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGAc                                                                                                                  <  3:142604/148‑1 (MQ=255)                               cTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCt                                                                                                                >  3:62785/1‑149 (MQ=255)                                ttGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCCGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTg                                                                                                               >  8:162137/1‑149 (MQ=255)                                   gCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGgc                                                                                                             <  3:1041/148‑1 (MQ=255)                                         gCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCcgcg                                                                                                      >  4:38066/1‑149 (MQ=255)                                         gCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCcgcg                                                                                                      >  5:230770/1‑149 (MQ=255)                                           cTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGcggt                                                                                                    >  3:295542/1‑149 (MQ=255)                                              aaCAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGcggtcg                                                                                                  <  6:212372/148‑1 (MQ=255)                                              aaCAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGcggtcg                                                                                                  <  7:162137/148‑1 (MQ=255)                                              aaCAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGcggtc                                                                                                   <  3:38066/147‑1 (MQ=255)                                               aCAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGcggtcggt                                                                                                <  1:167614/149‑1 (MQ=255)                                                     tAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGcac                                                                                          <  8:53673/149‑1 (MQ=255)                                                       gCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGcacca                                                                                        <  5:157564/149‑1 (MQ=255)                                                          ggCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCg                                                                                     >  6:80755/1‑149 (MQ=255)                                                              aGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAAt                                                                                 <  4:62785/149‑1 (MQ=255)                                                                    tCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGcc                                                                           >  3:38515/1‑149 (MQ=255)                                                                    tCGATGAGGGCCAGCTCGATACGGCTGACAAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGACCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCTCCACCGGAATGTCgac                                                                           >  3:42512/1‑147 (MQ=255)                                                                      gATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGcggtcg                                                                                                  >  3:220749/1‑124 (MQ=255)                                                                      gATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGcggtcg                                                                                                  <  4:220749/124‑1 (MQ=255)                                                                       aTGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGcca                                                                                                                                              >  8:2577/1‑79 (MQ=255)                                                                       aTGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGcca                                                                                                                                              <  7:2577/79‑1 (MQ=255)                                                                           gggCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCAc                                                                     >  3:14450/1‑148 (MQ=255)                                                                                aGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACcagcag                                                               <  3:240060/149‑1 (MQ=255)                                                                                 gCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAgg                                                              >  1:221902/1‑149 (MQ=255)                                                                                   tCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCgg                                                                        <  1:13847/137‑1 (MQ=255)                                                                                   tCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCgg                                                                        >  2:13847/1‑137 (MQ=255)                                                                                   tCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTg                                                            >  1:316462/1‑149 (MQ=255)                                                                                       tACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCTGCTGGTGccgg                                                        <  5:80755/149‑4 (MQ=255)                                                                                         cGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCTGCTGGTGccgggc                                                      <  4:208447/149‑6 (MQ=255)                                                                                         cGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGc                                                      <  2:316462/149‑1 (MQ=255)                                                                                                ccAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCg                                               >  2:62569/1‑149 (MQ=255)                                                                                                            acgcCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTg                                    >  1:280909/1‑149 (MQ=255)                                                                                                             cgcCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTgg                                   <  2:221902/149‑1 (MQ=255)                                                                                                              gcCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTggg                                  >  1:182435/1‑149 (MQ=255)                                                                                                               ccAACGCCCAGCCCGCGCACATCCAGCCGCAGGTAGTCCACCACCTGCCCGGGGCCATGGTAGGCGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCACGTGCTCGCCCTTGCCGGCCTGCCCCTGGGt                                 <  4:42512/149‑1 (MQ=255)                                                                                                               ccAAAGCCCCGCCGCCGCACATCCCGCAGCAGGTAGGCCACCCGCAGCCCGGGGCCATGGGAGGTGACCTGGCCGCCGCGGTCGGTCTGCCCCACCGGAATGTCGCCCGGCACCAGCAGGTGCGCGGCCTTGCCGGCCTGCCCCTGGGt                                 <  4:38515/149‑1 (MQ=255)                                                                                                                           cGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAgg                     <  2:182435/149‑1 (MQ=255)                                                                                                                                        gcagcagGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGAccagc        >  8:116872/1‑149 (MQ=255)                                                                                                                                           gcagGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGAccagc        >  8:22483/1‑146 (MQ=255)                                                                                                                                             agGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGAt   <  4:14450/149‑1 (MQ=255)                                                                                                                                              ggTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGAtt  <  4:204765/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                TCCGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCA‑CGCACGCCAAAGCCCAGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGATT  >  NC_002947/5464719‑5465007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑