Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 513,459 A→T 10.6% intergenic (‑106/‑85) crp ← / → yhfA DNA‑binding transcriptional dual regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947513,4590AT10.6% 146.7 / 5.5 54intergenic (‑106/‑85)crp/yhfADNA‑binding transcriptional dual regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (22/26);  new base T (3/3);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATGTTCTTTATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGCAATGTTCCTCGGCGAATCGGGGAGCGGCC  >  NC_002947/513314‑513604
                                                                                                                                                 |                                                                                                                                                 
cGATGTTCTTTATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTAATTTGGCGACAGCTTATAAGACACTACTAGATGTTTCGGTACt                                                                                                                                                 >  8:206600/1‑148 (MQ=255)
      tCTTTATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGa                                                                                                                                          >  3:197403/1‑149 (MQ=255)
       cTTTATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGa                                                                                                                                          >  2:215277/1‑148 (MQ=255)
        tttATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGaca                                                                                                                                        >  4:219759/1‑149 (MQ=255)
          tATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGac                                                                                                                                         <  4:197403/146‑1 (MQ=255)
          tATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACACATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGacac                                                                                                                                       <  3:252889/148‑1 (MQ=255)
                ggCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACAtctc                                                                                                                                  <  1:215277/147‑1 (MQ=255)
                 gCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGt                                                                                                                                <  3:219759/148‑1 (MQ=255)
                 gCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACAACGAGATGTGTCGGTTTAGTACCGAAACATCTCGta                                                                                                                               <  8:84847/149‑2 (MQ=38)
                        gggCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTc                                                                                                                         <  2:236173/148‑1 (MQ=255)
                             gAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAg                                                                                                                   >  3:279747/1‑149 (MQ=255)
                                   aCCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGca                                                                                                              >  3:299575/1‑148 (MQ=255)
                                   aCCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGATTTTCCCACAGCCGca                                                                                                              >  5:63466/1‑148 (MQ=255)
                                             aaTCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGTTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAg                                                                                                   <  1:128993/149‑1 (MQ=255)
                                             aaTCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAg                                                                                                   >  1:116997/1‑149 (MQ=255)
                                               tCCTGTTCGATATGACTCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCATTTCCCACAGCCGCACAAGGTCTAAGCt                                                                                                 <  1:178143/149‑1 (MQ=255)
                                               tCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCt                                                                                                 <  2:116997/149‑1 (MQ=255)
                                                  tGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGa                                                                                               >  7:73157/1‑148 (MQ=255)
                                                       gATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGac                                                                                              <  7:143914/144‑1 (MQ=255)
                                                       gATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGac                                                                                              >  8:143914/1‑144 (MQ=255)
                                                       gATATGACGCATAGGCTTTTTTAGAGGGTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACAccc                                                                                          <  2:268707/148‑1 (MQ=255)
                                                          atGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCttt                                                                                       <  6:63466/148‑1 (MQ=255)
                                                              cGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCg                                                                                   >  5:61810/1‑148 (MQ=255)
                                                               gCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGa                                                                                  >  7:4794/1‑148 (MQ=255)
                                                                       ttttttAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAg                                                                           >  4:15255/1‑147 (MQ=255)
                                                                            tAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTcc                                                                     <  3:15255/148‑1 (MQ=255)
                                                                            tAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTcc                                                                     <  4:279747/148‑1 (MQ=255)
                                                                                 gTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAg                                                               <  8:73157/149‑1 (MQ=255)
                                                                                     gCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATg                                                            >  8:271324/1‑148 (MQ=255)
                                                                                     gCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATg                                                            >  6:156179/1‑148 (MQ=255)
                                                                                      cTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATg                                                            >  1:45481/1‑147 (MQ=255)
                                                                                        gACGGAGAGCGCCAGGCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAgg                                                        <  8:121274/149‑1 (MQ=255)
                                                                                         aCGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAg                                                         <  8:4794/147‑1 (MQ=255)
                                                                                          cGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGc                                                       <  5:156179/148‑1 (MQ=255)
                                                                                                    cAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGt                                            <  7:271324/149‑1 (MQ=255)
                                                                                                     aGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCGAGTg                                           <  7:206600/149‑1 (MQ=255)
                                                                                                       tCATTTGTCTCCAGCTTATCAGACACTACGTGATGTTTCGGTAGTATAGCGACACATGTAGTGCTTTTGCCTCAGCCGGACAAGGTCTAAGCTGAGTCCCTTTTTCTTAATTCAGGAGTCCGGGTAGATGAAGGCAGGTATCGAGTgg                                          <  2:8526/148‑1 (MQ=255)
                                                                                                       tCATTTGGCGCCAGCTTATCAGACACAACGAGATTATTCGGTACTAAACAGAAACATCTCGTGCTTTTCCCACAGCCGCAAAACGTCTAAGCTGACACCCTTTTCCGAAAATAAGGATTCCGGCAAGATGAAGGCACGTATCAAGttt                                          >  1:8526/1‑146 (MQ=255)
                                                                                                                    gCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGCAAt                            >  8:235114/1‑149 (MQ=255)
                                                                                                                         tCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGCAATGTTcc                       >  7:268504/1‑149 (MQ=255)
                                                                                                                             acacTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGCAATGTTCCTCgg                   <  2:45481/149‑1 (MQ=255)
                                                                                                                                 tACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGc                               <  4:196845/133‑1 (MQ=255)
                                                                                                                                 tACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGc                               >  3:196845/1‑133 (MQ=255)
                                                                                                                                 caCGAGATGTGTCGGTTTAGTACCGAAACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAATATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAg                                 <  1:73747/130‑1 (MQ=38)
                                                                                                                                 caCGAGATGTGTCGGTTTAGTACCGAAACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAg                                 <  1:69242/130‑1 (MQ=38)
                                                                                                                                 caCGAGATGTGTCGGTTTAGTACCGAAACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAg                                 >  2:73747/2‑131 (MQ=38)
                                                                                                                                 caCGAGATGTGTCGGTTTAGTACCGAAACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAg                                 >  2:69242/2‑131 (MQ=38)
                                                                                                                                   cGAGATGTGTCGGTTTAGTACCGAAACATCTCGTGCTTTTCCCACAGCCGCACAAGATCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACATATCCAGTGGGCCGGTGAGGCAATGTTCCTCGGCGAATc             >  5:222950/1‑149 (MQ=255)
                                                                                                                                      gaTGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGCAATGTTCCTCGGCGAATCggg          <  6:61810/149‑1 (MQ=255)
                                                                                                                                       aTGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGc                                        <  4:112578/118‑1 (MQ=255)
                                                                                                                                       aTGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGc                                        >  3:112578/1‑118 (MQ=255)
                                                                                                                                              ggTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAgg                                >  1:306699/1‑119 (MQ=255)
                                                                                                                                              ggTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAgg                                <  2:306699/119‑1 (MQ=255)
                                                                                                                                              ggTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGCAATGTTCCTCGGCGAATCGGGGAGCGGcc  <  2:20568/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CGATGTTCTTTATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGCAATGTTCCTCGGCGAATCGGGGAGCGGCC  >  NC_002947/513314‑513604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑