Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,503,148 C→A 9.1% P33H (CCT→CAT) ‡ oprC → copper receptor OprC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,503,1480CA 9.1% 200.4 / 8.6 66P33H (CCT→CAT) ‡oprCcopper receptor OprC
Reads supporting (aligned to +/- strand):  ref base C (27/33);  new base A (2/4);  total (29/37)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01

ACGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGCCGACTACC  >  NC_002947/5503000‑5503291
                                                                                                                                                    |                                                                                                                                               
aCGAAGCCAGGAGGCCGCGCGCTAACTCATCCCTGATCGACTGGACTCATTATGTCCGCCTGCACCCCTGTTTTTGCCTCGTCCCAGCGTGGGACCATCGCCCCCCTGCGCGGCTCGCCGCCCGCGCCCATGGCCCTGGCCGCCGGccc                                                                                                                                                 <  4:193315/149‑1 (MQ=255)
    aGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTACACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGc                                                                                                                                             <  3:269134/149‑1 (MQ=255)
     gCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGcc                                                                                                                                            <  4:207532/149‑1 (MQ=255)
     gCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGcc                                                                                                                                            <  7:274475/149‑1 (MQ=255)
      ccAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCa                                                                                                                                           >  3:293589/1‑149 (MQ=255)
       cAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTTCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCAt                                                                                                                                          <  5:304188/149‑1 (MQ=255)
       cAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCAt                                                                                                                                          <  4:224120/149‑1 (MQ=255)
       cAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCAt                                                                                                                                          <  2:5085/149‑1 (MQ=255)
         ggaggCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCAGGGCCGGCGGCCAGGGCCATgg                                                                                                                                        <  3:85085/149‑2 (MQ=255)
            ggCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGa                                                                                                                                     <  1:212926/149‑1 (MQ=255)
             gCTTCGATCTAAATCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTTCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCTCCGGCCCTGGCCATTAAGa                                                                                                                                     <  8:250456/148‑1 (MQ=255)
              cTTCGCGCTAACTCATGACTGACCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGAcc                                                                                                                                   <  8:81717/149‑1 (MQ=255)
                 cgcgCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCAGGGCCGGCGGCCAGGGCCATgggcgcga                                                                                                                                  <  6:161783/147‑8 (MQ=38)
                    gcTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGc                                                                                                                             <  4:120410/149‑1 (MQ=255)
                        aCTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCacga                                                                                                                         >  2:249944/1‑149 (MQ=255)
                          tCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCAGGGCCGGCGGCCAGGGCCATggg                                                                                                                                       <  7:294210/133‑3 (MQ=255)
                          tCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCAGGGCCGGCGGCCAGGGCCATggg                                                                                                                                       <  7:294208/133‑3 (MQ=255)
                          tCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCAGGGCCGGCGGCCAGGGCCATggg                                                                                                                                       >  8:294208/1‑131 (MQ=255)
                          tCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCAGGGCCGGCGGCCAGGGCCATggg                                                                                                                                       >  8:294210/1‑131 (MQ=255)
                           cATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCacgacg                                                                                                                       >  1:187085/1‑148 (MQ=255)
                                    tCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGtt                                                                                                             <  7:175039/149‑1 (MQ=255)
                                        cTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCTCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGc                                                                                                         <  4:306421/149‑1 (MQ=255)
                                         tGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGa                                                                                                                                     <  1:171373/120‑1 (MQ=255)
                                         tGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGa                                                                                                                                     >  2:171373/1‑120 (MQ=255)
                                           gAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGcc                                                                                                        <  4:293589/147‑1 (MQ=255)
                                           gAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGc                                                                                                         >  8:239840/1‑146 (MQ=255)
                                           gAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGc                                                                                                         <  7:239840/146‑1 (MQ=255)
                                                 ttATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGt                                                                                                 >  7:77899/1‑148 (MQ=255)
                                                 ttATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGACTTGAGCCCGACGGt                                                                                                 >  4:4812/1‑148 (MQ=255)
                                                 ttATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATAGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGCCCATGTGCACGACGCACCCGACATGAGCCCGACGGt                                                                                                 >  2:138747/1‑148 (MQ=255)
                                                         ggCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCAccgc                                                                                        >  3:170909/1‑149 (MQ=255)
                                                          gCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGCCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCAccgcc                                                                                       <  1:249944/149‑1 (MQ=255)
                                                               aCCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGc                                                                                  <  4:170909/149‑1 (MQ=255)
                                                                 cccTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCAGCCGAGTTGAGCCCGACGGTGATCACCGCCGTGAccc                                                                                >  5:302645/1‑149 (MQ=255)
                                                                  ccTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGcccc                                                                               <  2:187085/149‑1 (MQ=255)
                                                                   cTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGa                                                                                                           <  1:273200/120‑1 (MQ=255)
                                                                   cTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGa                                                                                                           >  2:273200/1‑120 (MQ=255)
                                                                    tGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGcccc                                                                               >  8:127113/1‑147 (MQ=255)
                                                                         ttGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTc                                                                         <  2:248724/148‑1 (MQ=255)
                                                                           gCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATTGCCCTGGCCGCCGGCCCTGGCCATGAATACCATGTCACCCACGCACCCGAGTTGAGCCCGCCCGTGCTCACAGCAGATGCCCCGAGTAcgcc                                                                      >  5:296574/1‑149 (MQ=255)
                                                                                gTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGa                                                                 >  2:46685/1‑149 (MQ=255)
                                                                                      gCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTgg                                                           >  8:254520/1‑149 (MQ=255)
                                                                                             aCCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCaa                                                    >  8:32168/1‑149 (MQ=255)
                                                                                               cATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAAcc                                                  >  3:228765/1‑149 (MQ=255)
                                                                                                    ccgccCTGTGCGGCTCGCTGCTCGCGCCTATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGa                                               >  6:237015/1‑147 (MQ=255)
                                                                                                    ccgccCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGa                                               >  5:262888/1‑147 (MQ=255)
                                                                                                        ccTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGAcc                                         >  7:247465/1‑149 (MQ=255)
                                                                                                            tgCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCcgc                                      <  7:127113/148‑1 (MQ=255)
                                                                                                            tgCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCcgc                                      <  8:247465/148‑1 (MQ=255)
                                                                                                               ggCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgcca                                  <  7:32168/149‑1 (MQ=255)
                                                                                                                 cTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgccagc                                <  4:207952/149‑1 (MQ=255)
                                                                                                                    gctgctCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgccagcca                              <  4:228765/148‑1 (MQ=255)
                                                                                                                    gctgctCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgccagcc                               <  8:77899/147‑1 (MQ=255)
                                                                                                                         tCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCg                        >  5:119764/1‑149 (MQ=255)
                                                                                                                           gcgcCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCcg                                       <  4:60057/132‑1 (MQ=255)
                                                                                                                           gcgcCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCcg                                       >  3:60057/1‑132 (MQ=255)
                                                                                                                                    gCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACgg             <  3:4812/149‑1 (MQ=255)
                                                                                                                                     cccTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGgc            <  1:46685/149‑1 (MQ=255)
                                                                                                                                      ccTGGCCGCCGGCCCTGGACATGAAGTCCATGTGTAGGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGgcg           <  6:296574/149‑1 (MQ=255)
                                                                                                                                        tGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGcc         >  8:149626/1‑149 (MQ=255)
                                                                                                                                             gccgGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTc                                                                         <  4:145420/80‑1 (MQ=255)
                                                                                                                                             gccgGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTc                                                                         >  3:145420/1‑80 (MQ=255)
                                                                                                                                             gccgGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGCCGACTa    >  6:217537/1‑149 (MQ=255)
                                                                                                                                             gccgGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGCCGACTa    >  2:35095/1‑149 (MQ=255)
                                                                                                                                              ccgGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGCCGACTAc   <  7:254520/149‑1 (MQ=255)
                                                                                                                                              ccgGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGCCGACTAc   <  1:35095/149‑1 (MQ=255)
                                                                                                                                               cgGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGCCGACTAcc  >  4:129429/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
ACGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGCACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGCCGACTACC  >  NC_002947/5503000‑5503291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑