Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,529,189	A→C	6.1%	intergenic (‑403/+191)	ydfG ← / ← livF‑II	bifunctional NADP‑dependent 3‑hydroxy acid dehydrogenase/malonic semialdehyde reductase/ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,529,189	0	A	C	6.1%	197.1 / 2.1	62	intergenic (‑403/+191)	ydfG/livF‑II	bifunctional NADP‑dependent 3‑hydroxy acid dehydrogenase/malonic semialdehyde reductase/ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (29/29);  new base C (2/2);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATGCCGCCTGCAGCGGTCTGTCCAAGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTT  >  NC_002947/5529044‑5529336                                                                                                                                                   |                                                                                                                                                    aCATGCCGCCTGCAGCGGTCTGTCCAAGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAg                                                                                                                                                  >  3:184652/1‑149 (MQ=255)                cGGTCTGTCCAAGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAg                                                                                                                                   <  4:184652/149‑1 (MQ=255)                  gTCTGTCCAAGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGc                                                                                                                                 >  6:53402/1‑149 (MQ=255)                         cAAGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCtt                                                                                                                              >  6:312099/1‑145 (MQ=255)                         cAAGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCtt                                                                                                                              <  5:312099/145‑1 (MQ=255)                         cAAGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAg                                                                                                                           >  3:169891/1‑148 (MQ=255)                         cAAGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGc                                                                                                                          <  4:165569/149‑1 (MQ=255)                         cAAGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGc                                                                                                                          >  2:1618/1‑149 (MQ=255)                         cAAGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGc                                                                                                                          <  4:161341/149‑1 (MQ=255)                          aaGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGc                                                                                                                          >  8:14724/1‑148 (MQ=255)                           aGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGTACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGa                                                                                                                        >  1:56089/1‑149 (MQ=255)                             gTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGata                                                                                                                      <  1:309095/149‑1 (MQ=255)                                  tACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTCAAGGCCTTGAGTATCAAGCCCTGTAGCGATTTgat                                                                                                                  <  6:62074/148‑3 (MQ=37)                                       agagTTATCCACAGGATTGGCACTGCACTTGAAGCACCCGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATg                                                                                                            >  7:292591/1‑149 (MQ=255)                                            tATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAAC‑AAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTc                                                                                                       <  6:307621/148‑1 (MQ=255)                                             aTCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACACCGAATTTGAAAAAAAAATGAACAAAACGGTCCAGGGATTGATAAACATGGCCTTGAGCGTTAT‑CCACCAtttttttc                                                                                                      >  1:98637/1‑147 (MQ=255)                                                cacaGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCca                                                                                                     >  5:1149/1‑147 (MQ=255)                                                cacaGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCca                                                                                                     >  7:126882/1‑147 (MQ=255)                                                cacaGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCA‑AAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGa                                                                                                                                           >  6:327306/1‑108 (MQ=255)                                                cacaGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCA‑AAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGa                                                                                                                                           <  5:327306/108‑1 (MQ=255)                                                    ggATTGGCACTGCAATTGAAGCACACGCAGAGATTTTTAAAGCTTTGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAggcg                                                                                               <  3:198741/149‑1 (MQ=255)                                                            actgcactTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCTCTACAGGGCTTGATACTCAAGTCCTTGAGCGATATGCAACAATGTTTTACACAGGCGGCTCcac                                                                                        >  4:13213/1‑148 (MQ=255)                                                            actgcactTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCcaca                                                                                       >  8:50936/1‑149 (MQ=255)                                                            actgcactTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCcaca                                                                                       >  1:129034/1‑149 (MQ=255)                                                              tgcactTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCg                                                                                                                         >  4:180822/1‑113 (MQ=255)                                                              tgcactTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCg                                                                                                                         <  3:180822/113‑1 (MQ=255)                                                                            acGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAaga                                                                       <  1:1618/149‑1 (MQ=255)                                                                            acGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAaga                                                                       <  8:126882/149‑1 (MQ=255)                                                                                       tttaaaGCTTGGTAAAATCATCCACAAA‑GGGTATATTAACAAAAAATGATCAAATCGCTACAGGGCTTTATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCtgt                                                             <  3:13213/145‑1 (MQ=255)                                                                                       ttGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGt                                                                     <  3:146702/140‑1 (MQ=255)                                                                                       ttGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGt                                                                     >  4:146702/1‑140 (MQ=255)                                                                                       ttGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCtgtg                                                            <  6:126917/149‑1 (MQ=255)                                                                                       ttGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCtgt                                                             <  6:1149/148‑1 (MQ=255)                                                                                        tGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCtgtg                                                            >  6:75517/1‑148 (MQ=255)                                                                                        tGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCtgtg                                                            <  5:75517/148‑1 (MQ=255)                                                                                         gAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATTCCACCATGTTTTCCACAGGCGGATCCACACTATCCGTGGAAAAGAGTTGGCCTGTgg                                                           >  7:43261/1‑148 (MQ=255)                                                                                           aaGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGaaa                                                        <  2:56089/149‑1 (MQ=255)                                                                                            agattGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATGAATTCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACAATGTTTTCCACAGGCGCCTCCACACTATCCGt                                                                               >  1:65692/4‑125 (MQ=255)                                                                                            aGCTTGGGAAATTCATCCACAAC‑CGGGATATGAACAAAAAATGATCAAATCTCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGt                                                                               <  2:65692/125‑1 (MQ=255)                                                                                            aGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGt                                                                               >  1:70229/1‑125 (MQ=255)                                                                                            aGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGt                                                                               <  2:70229/125‑1 (MQ=255)                                                                                              cTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGGTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCAGTGGAAAAGAGTTGGCCTGTGGAAACaa                                                     >  6:286854/1‑149 (MQ=255)                                                                                                 gggAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAAt                                                    <  4:169891/147‑1 (MQ=255)                                                                                                          atcCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGtt                                                                    >  6:182733/1‑122 (MQ=255)                                                                                                          atcCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGtt                                                                    <  5:182733/122‑1 (MQ=255)                                                                                                          atcCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTAGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGaa                                          <  6:143211/148‑1 (MQ=255)                                                                                                            ccACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCtt                                               <  4:300121/141‑1 (MQ=255)                                                                                                            ccACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCtt                                               >  3:300121/1‑141 (MQ=255)                                                                                                                aaC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAatat                                   <  5:53402/149‑1 (MQ=255)                                                                                                                  c‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATgg                                 <  7:14724/149‑1 (MQ=255)                                                                                                                  c‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATgg                                 <  8:43261/149‑1 (MQ=255)                                                                                                                              aCAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGa                                       <  2:243181/132‑1 (MQ=255)                                                                                                                              aCAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGa                                       >  1:243181/1‑132 (MQ=255)                                                                                                                              aCAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCgg                      >  3:248375/1‑149 (MQ=255)                                                                                                                              aCAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCgg                      >  8:237710/1‑149 (MQ=255)                                                                                                                               cAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCgg                      >  8:258709/1‑148 (MQ=255)                                                                                                                                   aaaTGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGaa                 <  7:237710/149‑1 (MQ=255)                                                                                                                                   aaaTGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGaa                 <  4:248375/149‑1 (MQ=255)                                                                                                                                      tGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAgg               >  5:91765/1‑148 (MQ=255)                                                                                                                                         gcataTCGCTCAAGGCCTTGAGTATCAAGCCCTGTAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAaga                                                                       <  7:216213/85‑1 (MQ=17)                                                                                                                                         gcataTCGCTCAAGGCCTTGAGTATCAAGCCCTGTAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAaga                                                                       >  8:216213/5‑89 (MQ=17)                                                                                                                                                  tACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGttt  >  8:260575/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                    ACATGCCGCCTGCAGCGGTCTGTCCAAGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAAC‑GGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTGAGCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTT  >  NC_002947/5529044‑5529336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑