Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,561,959 T→A 7.4% D309V (GAC→GTC)  hflK ← integral membrane ATP‑dependent zinc metallopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,561,9590TA 7.4% 161.6 / 2.7 54D309V (GAC→GTC) hflKintegral membrane ATP‑dependent zinc metallopeptidase
Reads supporting (aligned to +/- strand):  ref base T (27/23);  new base A (2/2);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

TAGAGCAGGTTGCTCTGCCCGTCCTTGGTTGCCACCATGACCTTGCTCGAGTTGCTGTAGACCTCTTGCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGC‑ACCACGCCATTGGCATAGGACTCGGCCTGGTTGCGGGCACGCTGCTC  >  NC_002947/5561815‑5562095
                                                                                                                                                |                                                                                                                                         
tAGAGCAGGTTGCTCTGCCCGTCCTTGGTTGCCACCATGACCTTGCTCGAGTTGCTGTAGACCTCTTGCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGc                                                                                                                                       <  8:21281/149‑1 (MQ=255)
      aGGTTGCTCTGCCCGTCCTTGGTTGCCACCATGACCTTGCTCGAGTTGCTGTAGACCTCTTGCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGc                                                                                                                                       <  3:34374/143‑1 (MQ=255)
      aGGTTGCTCTGCCCGTCCTTGGTTGCCACCATGACCTTGCTCGAGTTGCTGTAGACCTCTTGCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGc                                                                                                                                       >  4:34374/1‑143 (MQ=255)
                gCCCGTCCTTGGTTGCCACCATGACCTTGCTCGAGTTGCTGTAGACCTCTTGCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCgg                                                                                                                       >  1:313427/1‑149 (MQ=255)
                 cccGTCCTTGGTTGCCACCATGACCTTGCTCGAGTTGCTGTAGACCTCTTGCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCgg                                                                                                                       <  3:147296/148‑1 (MQ=255)
                  ccGTCCTTGGTTGCCACCATGACCTTGCTCGAGTTGCTGTAGACCTCTTGCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGACAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCggg                                                                                                                      >  7:39404/1‑148 (MQ=255)
                    gTCCTTGGTTGCCACCATGACCTTGCTCGAGTTGCTGTAGACCTCTTGCATGGTCTCCAGATACAGACGCTCTCGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCt                                                                                                                                     <  8:58076/131‑1 (MQ=255)
                    gTCCTTGGTTGCCACCATGACCTTGCTCGAGTTGCTGTAGACCTCTTGCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCt                                                                                                                                     >  7:58076/1‑131 (MQ=255)
                       gttGGTTGCCACCATTACATTGCTCGAGTTGCTGTAGACCTCTTCCATGGTCTCCACTTACCGACGCTCACGGGTGACGTCTGGTGCCTTGCTGTTCTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGa                                                                                                                <  4:281849/148‑1 (MQ=255)
                          ggTTGCCACCATGACCTTGCTCGAGTTGCTGTAGACCTCTTGCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACt                                                                                                              <  2:313427/148‑1 (MQ=255)
                                caccaTGACCTTGCTCGAGTTGTTGTAGACCTCTTGCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTcgc                                                                                                       >  6:212771/1‑149 (MQ=255)
                                caccaTGACCTTGCTCGAGTTGCTGTAGACCTCTTGCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTg                                                                                                                             <  3:207569/127‑1 (MQ=255)
                                caccaTGACCTTGCTCGAGTTGCTGTAGACCTCTTGCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTg                                                                                                                             >  4:207569/1‑127 (MQ=255)
                                 accaTGACCTTGCTCGAGTTGCTGTAGACCTCTTGCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGACAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTcgcg                                                                                                      <  8:39404/149‑1 (MQ=255)
                                            gCTCGAGTTGCTGTAGACCTCTTGCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGACTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTgg                                                                                           >  1:214108/1‑149 (MQ=255)
                                              tCGAGTTGCTGTAGACCTCTTGCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCa                                                                                         >  2:50311/1‑149 (MQ=255)
                                                      cTGTAGACCTCTTGCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGACTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCga                                                                                  <  2:214108/148‑1 (MQ=255)
                                                         tAGACCTCTTGCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCTATGACTTCGTCGCGGTAACCATTgg                                                                                           <  1:118802/136‑1 (MQ=255)
                                                         tAGACCTCTTGCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCGATGACTTCGTCGCGGTAACCATTgg                                                                                           >  2:118802/1‑136 (MQ=255)
                                                           gACCTCTTGCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGatgatg                                                                             <  5:212771/148‑1 (MQ=255)
                                                            aCCTCTTGCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATgcg                                                                           >  4:210891/1‑149 (MQ=255)
                                                                 ttGCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTggg                                                                      >  2:37893/1‑149 (MQ=255)
                                                                   gCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGcc                                                                    >  4:238464/1‑149 (MQ=255)
                                                                     aTGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCaa                                                                                                                   >  4:146063/1‑100 (MQ=255)
                                                                     aTGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCaa                                                                                                                   <  3:146063/100‑1 (MQ=255)
                                                                             cAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGc                                                                                          >  3:247777/1‑117 (MQ=255)
                                                                             cAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGc                                                                                          <  4:247777/117‑1 (MQ=255)
                                                                             cAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACgg                                                           <  3:210891/148‑1 (MQ=255)
                                                                                    ataCGCTCACGGGTTACGTCAGGTGGCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGTCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCGTCGAGTATGCGCTGGGCCTGACCACGGGCTTCCgg                                                   <  8:158268/147‑1 (MQ=255)
                                                                                    aGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCgg                                                   >  2:279064/1‑149 (MQ=255)
                                                                                         cTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGc‑acca                                             <  1:267609/149‑1 (MQ=255)
                                                                                          tCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGc‑accac                                            >  5:270843/1‑149 (MQ=255)
                                                                                          tCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGc‑accac                                            >  7:97603/1‑149 (MQ=255)
                                                                                          tCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGc‑accac                                            >  1:91534/1‑149 (MQ=255)
                                                                                            aCGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGc‑accac                                            >  6:160555/1‑147 (MQ=255)
                                                                                            aCGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGc‑accac                                            <  5:160555/147‑1 (MQ=255)
                                                                                            aCGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGC‑ACCACGc                                          >  3:220285/1‑149 (MQ=255)
                                                                                            aCGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGC‑ACCACGc                                          <  3:238464/149‑1 (MQ=255)
                                                                                                gTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTg                                                                        >  6:307702/1‑116 (MQ=255)
                                                                                                gTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTg                                                                        <  5:307702/116‑1 (MQ=255)
                                                                                                    cGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGC‑ACCACGCCATTGGc                                   <  6:270843/148‑1 (MQ=255)
                                                                                                     gTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGC‑ACCACGCCATTGGCa                                  >  3:107792/1‑148 (MQ=255)
                                                                                                     gTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGC‑ACCACGCCATTGGCa                                  >  7:118164/1‑148 (MQ=255)
                                                                                                     gTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGC‑ACCACGCCATTGGCa                                  >  7:138326/1‑148 (MQ=255)
                                                                                                                   ggTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGC‑ACCACGCCATTGGCATAGGACTCGGCCTgg                   >  5:46603/1‑149 (MQ=255)
                                                                                                                    gTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGC‑ACCACGCCATTg                                     <  2:55624/130‑1 (MQ=255)
                                                                                                                       cTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGCAACCACGCCATTGGCATAGGACTCGGCCTGGtt                 <  1:37893/148‑1 (MQ=255)
                                                                                                                        tCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGC‑ACCACGCCATTGGCATAGGACTCGGCCTGGTTGCg              >  7:46488/1‑149 (MQ=255)
                                                                                                                          ggCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGC‑ACCACGCCATTGGCATAGGACTCGGCCTGGTTGCgg             <  8:97603/148‑1 (MQ=255)
                                                                                                                           gCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGC‑ACCACGCCATTGGCATAGGACTCGGCCTGGTTGCGGGc           <  8:118164/149‑1 (MQ=255)
                                                                                                                               gcagcTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGC‑ACCACGCCATTGGCATAGGACTCGGCCTGGTTGCGGGCACgc       >  6:120624/1‑149 (MQ=255)
                                                                                                                                cagcTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGC‑ACCACGCCATTGGCATAGGACTCGGCCTGGTTGCGGGCACgct      >  7:163245/1‑149 (MQ=255)
                                                                                                                                   aagggTGAGGCAGACCGCTTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGC‑ACCACGCCATTGGCATAGGACTCGGCCTGGTTGCGGGCACgctgct   >  1:311378/4‑149 (MQ=255)
                                                                                                                                    agggTGAGGCAGACCGCTTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGC‑ACCACGCCATTGGCATAGGACTCGGCCTGGTTGCGGGCACGCTGCTc  <  2:311378/147‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                         
TAGAGCAGGTTGCTCTGCCCGTCCTTGGTTGCCACCATGACCTTGCTCGAGTTGCTGTAGACCTCTTGCATGGTCTCCAGATACAGACGCTCACGGGTTACGTCAGGTGCCTTGCGGTACTCGGCAAGCAGCTTGGTGAAGCGGTCTGCCTCACCCTTGGCGCGGGCAATGACTTCGTCGCGGTAACCATTGGCATCCTCGATGATGCGCTGGGCCTGACCACGGGCTTCCGGC‑ACCACGCCATTGGCATAGGACTCGGCCTGGTTGCGGGCACGCTGCTC  >  NC_002947/5561815‑5562095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑