Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,570,177 T→G 7.5% A24A (GCA→GCC)  nnrD ← ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,570,1770TG 7.5% 185.7 / 4.6 64A24A (GCA→GCC) nnrDADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (25/34);  new base G (3/2);  total (28/36)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCGCCTC  >  NC_002947/5570031‑5570324
                                                                                                                                                  |                                                                                                                                                   
tGCTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCtgccgtgc                                                                                                                                                   <  5:103793/149‑1 (MQ=255)
   tCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACGTGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCgg                                                                                                                                                >  3:120554/1‑149 (MQ=255)
     gggCCGGGATGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTGCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCgg                                                                                                                                                <  6:253505/147‑1 (MQ=255)
          gggTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGTCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCgg                                                                                                                                         <  1:310133/149‑1 (MQ=255)
           ggTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCggg                                                                                                                                        >  3:70472/1‑149 (MQ=255)
            gTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCggg                                                                                                                                        >  7:234722/1‑148 (MQ=255)
             ttGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCa                                                                                                                                      <  4:10532/149‑1 (MQ=255)
                                  cgccgcAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAAt                                                                                                                 <  2:225033/149‑1 (MQ=255)
                                   gccgcAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGGACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAAtt                                                                                                                <  4:214647/149‑1 (MQ=255)
                                      gcAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATtgtg                                                                                                             <  6:89010/149‑1 (MQ=255)
                                      gcAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATtgtg                                                                                                             <  7:248040/149‑1 (MQ=255)
                                       cAACGCTGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGTTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTgg                                                                                                            <  3:230586/149‑1 (MQ=255)
                                       cAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTTCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTgg                                                                                                            <  5:49231/149‑1 (MQ=255)
                                       cAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTgg                                                                                                            <  1:88534/149‑1 (MQ=255)
                                        aaCGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACGTGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTggg                                                                                                           <  4:120554/149‑1 (MQ=255)
                                        aaCGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTggg                                                                                                           <  8:234722/149‑1 (MQ=255)
                                        aaCGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTggg                                                                                                           >  4:237602/1‑149 (MQ=255)
                                        aaCGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTggg                                                                                                           <  7:176336/149‑1 (MQ=255)
                                           gCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCa                                                                                                        >  2:206232/1‑149 (MQ=255)
                                              gggCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCAtt                                                                                                      >  7:261510/1‑148 (MQ=255)
                                                 cggcTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTg                                                                                                  <  2:216561/149‑1 (MQ=255)
                                                   gcTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTggg                                                                                                <  4:63696/149‑1 (MQ=255)
                                                     ttCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGgt                                                                                              >  8:118877/1‑149 (MQ=255)
                                                        ggCACTGAGCAACACAGCGCGTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGGATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGtt                                                                                           <  4:292911/149‑1 (MQ=255)
                                                         gCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGttt                                                                                          <  4:70472/149‑1 (MQ=255)
                                                           aCTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTg                                                                                         >  3:272159/1‑148 (MQ=255)
                                                           aCTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTg                                                                                         <  4:272159/148‑1 (MQ=255)
                                                               aGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTg                                                                                    >  3:282614/1‑149 (MQ=255)
                                                                gCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGc                                                                                   >  2:113009/1‑149 (MQ=255)
                                                                   acacAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCgg                                                                                 >  7:158873/1‑148 (MQ=255)
                                                                       aGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATgg                                                                            >  7:44169/1‑149 (MQ=255)
                                                                        gcgcCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATggg                                                                           >  7:91779/1‑149 (MQ=255)
                                                                          gcCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTACGGCATGGGGt                                                                         <  3:222292/149‑1 (MQ=255)
                                                                                ggTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAgg                                                                   <  7:118877/149‑1 (MQ=255)
                                                                                     ccAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATggg                                                                           <  6:20875/136‑1 (MQ=255)
                                                                                     ccAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATggg                                                                           >  5:20875/1‑136 (MQ=255)
                                                                                       aGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAAt                                                            >  8:96444/1‑149 (MQ=255)
                                                                                       aGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCAATACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAAt                                                            >  7:66757/1‑149 (MQ=255)
                                                                                       aGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGGGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAAt                                                            <  4:26431/149‑1 (MQ=255)
                                                                                               cccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCAATACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCa                                                    <  8:66757/149‑1 (MQ=255)
                                                                                                ccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTgg                                                      >  1:128420/1‑146 (MQ=255)
                                                                                                       ccaGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATtata                                            <  2:128420/149‑1 (MQ=255)
                                                                                                       ccaGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATtata                                            <  7:96444/149‑1 (MQ=255)
                                                                                                          gTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACg                                          >  3:125231/1‑148 (MQ=255)
                                                                                                              aTGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGTGTGTTTGGTCTGc                                                                                   <  8:118854/103‑1 (MQ=255)
                                                                                                              aTGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGc                                                                                   >  7:118854/1‑103 (MQ=255)
                                                                                                               tGGCCAAAGTCGCCTTTGTGGGCATGAGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGAGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATTTATTGCAGAATtataagaaact                                     >  6:221558/1‑141 (MQ=255)
                                                                                                                    aaaGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGccc                               >  5:140985/1‑149 (MQ=255)
                                                                                                                    aaaGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGccc                               >  2:131325/1‑149 (MQ=255)
                                                                                                                       gTCGCCTTTGTGGGCAGTCGCCTGGCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTa                            <  8:91779/149‑1 (MQ=255)
                                                                                                                          gCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTg                                                       <  7:147427/119‑1 (MQ=255)
                                                                                                                          gCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTg                                                       >  8:147427/1‑119 (MQ=255)
                                                                                                                          gCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAtt                          <  4:125231/148‑1 (MQ=255)
                                                                                                                            cTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgcc                       <  3:237602/149‑1 (MQ=255)
                                                                                                                                gtgGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAACGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgccttgc                   >  2:199364/1‑149 (MQ=255)
                                                                                                                                 tgGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgccttgc                   <  8:158873/148‑1 (MQ=255)
                                                                                                                                    gCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccat               <  6:140985/149‑1 (MQ=255)
                                                                                                                                    gCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccat               <  1:206232/149‑1 (MQ=255)
                                                                                                                                    gCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCcca                >  4:150273/1‑148 (MQ=255)
                                                                                                                                     cATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatc              <  3:150273/149‑1 (MQ=255)
                                                                                                                                        ccgcCTGCCGGCACGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGccc                               <  8:115233/128‑1 (MQ=255)
                                                                                                                                        ccgcCTGCCGGCACGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGccc                               >  7:115233/2‑129 (MQ=255)
                                                                                                                                        ccgcCTGCCGGCACGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatccat           >  2:84133/2‑149 (MQ=255)
                                                                                                                                        ccgcCTGCCGGCACGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatccat           >  3:128485/2‑149 (MQ=255)
                                                                                                                                         cgcCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATg          <  4:282614/149‑1 (MQ=255)
                                                                                                                                               ccgtgccgGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCGcc    >  6:316859/1‑149 (MQ=255)
                                                                                                                                                 gtgccgGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgccttgc                   <  5:44453/132‑1 (MQ=255)
                                                                                                                                                 gtgccgGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgccttgc                   >  6:44453/1‑132 (MQ=255)
                                                                                                                                                 gtgccgGCAGGCGGGCAACCCTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCGCctc  <  8:261510/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TGCTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCGCCTC  >  NC_002947/5570031‑5570324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑