Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 513,463 A→T 12.8% intergenic (‑110/‑81) crp ← / → yhfA DNA‑binding transcriptional dual regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947513,4630AT12.8% 130.5 / 8.6 53intergenic (‑110/‑81)crp/yhfADNA‑binding transcriptional dual regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (21/25);  new base T (3/4);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

TCTTTATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGCAATGTTCCTCGGCGAATCGGGGAGCGGCCATGTCGT  >  NC_002947/513320‑513611
                                                                                                                                               |                                                                                                                                                    
tCTTTATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGa                                                                                                                                                 >  3:197403/1‑149 (MQ=255)
 cTTTATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGa                                                                                                                                                 >  2:215277/1‑148 (MQ=255)
  tttATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGaca                                                                                                                                               >  4:219759/1‑149 (MQ=255)
    tATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGac                                                                                                                                                <  4:197403/146‑1 (MQ=255)
    tATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACACATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGacac                                                                                                                                              <  3:252889/148‑1 (MQ=255)
          ggCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACAtctc                                                                                                                                         <  1:215277/147‑1 (MQ=255)
           gCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGt                                                                                                                                       <  3:219759/148‑1 (MQ=255)
           gCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACAACGAGATGTGTCGGTTTAGTACCGAAACATCTCGta                                                                                                                                      <  8:84847/149‑2 (MQ=38)
                  gggCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTc                                                                                                                                <  2:236173/148‑1 (MQ=255)
                       gAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAg                                                                                                                          >  3:279747/1‑149 (MQ=255)
                             aCCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGca                                                                                                                     >  3:299575/1‑148 (MQ=255)
                             aCCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGATTTTCCCACAGCCGca                                                                                                                     >  5:63466/1‑148 (MQ=255)
                                       aaTCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGTTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAg                                                                                                          <  1:128993/149‑1 (MQ=255)
                                       aaTCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAg                                                                                                          >  1:116997/1‑149 (MQ=255)
                                         tCCTGTTCGATATGACTCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCATTTCCCACAGCCGCACAAGGTCTAAGCt                                                                                                        <  1:178143/149‑1 (MQ=255)
                                         tCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCt                                                                                                        <  2:116997/149‑1 (MQ=255)
                                            tGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGa                                                                                                      >  7:73157/1‑148 (MQ=255)
                                                 gATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGac                                                                                                     >  8:143914/1‑144 (MQ=255)
                                                 gATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGac                                                                                                     <  7:143914/144‑1 (MQ=255)
                                                 gATATGACGCATAGGCTTTTTTAGAGGGTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACAccc                                                                                                 <  2:268707/148‑1 (MQ=255)
                                                    atGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCttt                                                                                              <  6:63466/148‑1 (MQ=255)
                                                        cGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCg                                                                                          >  5:61810/1‑148 (MQ=255)
                                                         gCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGa                                                                                         >  7:4794/1‑148 (MQ=255)
                                                                 ttttttAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAg                                                                                  >  4:15255/1‑147 (MQ=255)
                                                                      tAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTcc                                                                            <  4:279747/148‑1 (MQ=255)
                                                                      tAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTcc                                                                            <  3:15255/148‑1 (MQ=255)
                                                                           gTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAg                                                                      <  8:73157/149‑1 (MQ=255)
                                                                               gCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATg                                                                   >  6:156179/1‑148 (MQ=255)
                                                                               gCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATg                                                                   >  8:271324/1‑148 (MQ=255)
                                                                                cTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATg                                                                   >  1:45481/1‑147 (MQ=255)
                                                                                  gACGGAGAGCGCCAGGCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAgg                                                               <  8:121274/149‑1 (MQ=255)
                                                                                   aCGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAg                                                                <  8:4794/147‑1 (MQ=255)
                                                                                    cGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGc                                                              <  5:156179/148‑1 (MQ=255)
                                                                                              cAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGt                                                   <  7:271324/149‑1 (MQ=255)
                                                                                               aGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCGAGTg                                                  <  7:206600/149‑1 (MQ=255)
                                                                                                 tCATTTGTCTCCAGCTTATCAGACACTACGTGATGTTTCGGTAGTATAGCGACACATGTAGTGCTTTTGCCTCAGCCGGACAAGGTCTAAGCTGAGTCCCTTTTTCTTAATTCAGGAGTCCGGGTAGATGAAGGCAGGTATCGAGTgg                                                 <  2:8526/148‑1 (MQ=255)
                                                                                                 tCATTTGGCGCCAGCTTATCAGACACAACGAGATTATTCGGTACTAAACAGAAACATCTCGTGCTTTTCCCACAGCCGCAAAACGTCTAAGCTGACACCCTTTTCCGAAAATAAGGATTCCGGCAAGATGAAGGCACGTATCAAGttt                                                 >  1:8526/1‑146 (MQ=255)
                                                                                                              gCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGCAAt                                   >  8:235114/1‑149 (MQ=255)
                                                                                                                   tCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGCAATGTTcc                              >  7:268504/1‑149 (MQ=255)
                                                                                                                       acacTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGCAATGTTCCTCgg                          <  2:45481/149‑1 (MQ=255)
                                                                                                                           tACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGc                                      <  4:196845/133‑1 (MQ=255)
                                                                                                                           tACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGc                                      >  3:196845/1‑133 (MQ=255)
                                                                                                                           caCGAGATGTGTCGGTTTAGTACCGAAACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAATATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAg                                        <  1:73747/130‑1 (MQ=38)
                                                                                                                           caCGAGATGTGTCGGTTTAGTACCGAAACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAg                                        >  2:69242/2‑131 (MQ=38)
                                                                                                                           caCGAGATGTGTCGGTTTAGTACCGAAACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAg                                        <  1:69242/130‑1 (MQ=38)
                                                                                                                           caCGAGATGTGTCGGTTTAGTACCGAAACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAg                                        >  2:73747/2‑131 (MQ=38)
                                                                                                                             cGAGATGTGTCGGTTTAGTACCGAAACATCTCGTGCTTTTCCCACAGCCGCACAAGATCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACATATCCAGTGGGCCGGTGAGGCAATGTTCCTCGGCGAATc                    >  5:222950/1‑149 (MQ=255)
                                                                                                                                gaTGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGCAATGTTCCTCGGCGAATCggg                 <  6:61810/149‑1 (MQ=255)
                                                                                                                                 aTGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGc                                               >  3:112578/1‑118 (MQ=255)
                                                                                                                                 aTGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGc                                               <  4:112578/118‑1 (MQ=255)
                                                                                                                                        ggTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAgg                                       >  1:306699/1‑119 (MQ=255)
                                                                                                                                        ggTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAgg                                       <  2:306699/119‑1 (MQ=255)
                                                                                                                                        ggTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGCAATGTTCCTCGGCGAATCGGGGAGCGGcc         <  2:20568/149‑1 (MQ=255)
                                                                                                                                               aaCCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGCAATGTTCCTCGGCGAATCGGGGAGCGGCCATgtcgt  >  7:192778/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
TCTTTATCTTGGCGGGTAGGGCGGAGGCAACCATCACGAAATCCTGTTCGATATGACGCATAGGCTTTTTTAGAGGTTGGCTGACGGAGAGCGCCAGTCATTTGGCGCCAGCTTATCAGACACTACGAGATGTTTCGGTACTAAACCGACACATCTCGTGCTTTTCCCACAGCCGCACAAGGTCTAAGCTGACACCCTTTTCCGAAAATCAGGAGTCCGGGTAGATGAAGGCACGTATCCAGTGGGCCGGTGAGGCAATGTTCCTCGGCGAATCGGGGAGCGGCCATGTCGT  >  NC_002947/513320‑513611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑