Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,645,307	C→T	9.2%	A220A (GCC→GCT)	PP_4954 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,645,307	0	C	T	9.2%	145.9 / 4.0	55	A220A (GCC→GCT)	PP_4954	membrane protein
Reads supporting (aligned to +/- strand):  ref base C (25/24);  new base T (3/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

CCGGTACAGCAGAGCCTGGCCAGACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAACGCACTGA  >  NC_002947/5645164‑5645455                                                                                                                                                |                                                                                                                                                      ccGGTACAGCAGAGCCTGGCCAGACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCt                                                                                                                                                  <  7:182327/149‑1 (MQ=255)   ggTACAGCAGAGCCTGGCCAGACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGa                                                                                                                                                <  4:288274/149‑1 (MQ=255)          cagAGCCTGGCCAGACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCa                                                                                                                                           >  6:9241/1‑147 (MQ=255)          cagAGCCTGGCCAGACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCa                                                                                                                                           <  5:9241/147‑1 (MQ=255)          cagAGCCTGGCCAGACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGc                                                                                                                                         >  5:17126/1‑149 (MQ=255)             agCCTGGCCAGACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCTGCGTTCAGCGCGGCC‑ac                                                                                                                                     <  5:106341/149‑2 (MQ=255)               ccTGGCCAGACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGCGGTGGCCGCGCTGAACGCAGCC‑AAgg                                                                                                                                   <  1:8638/149‑1 (MQ=255)                   gCCAGACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGgaga                                                                                                                                <  4:283318/148‑1 (MQ=255)                   gCCAGACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGgaga                                                                                                                                <  6:172980/148‑1 (MQ=255)                       gACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACTGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGatcatc                                                                                                                           <  8:287798/149‑1 (MQ=255)                        aCTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGgaga                                                                                                                                <  4:263112/143‑1 (MQ=255)                        aCTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGgaga                                                                                                                                >  3:263112/1‑143 (MQ=255)                         cTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATcc                                                                                                                          <  7:276147/148‑1 (MQ=255)                               ttcGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCg                                                                                                                   >  5:90306/1‑149 (MQ=255)                                   aGCTGCTCAGCTACGTGAAGCTCAAGTAAATCCTGTTTTTTACGGTCCGCTCGCTCGACGTCTAGGCAAGGCGCCTGGAACTGGCCCAGCAGAAGGGGAAGGTGGTGGAGGGGCTGATCGGTGCC‑AAGGAGATCTTCCTGGAGCGGGTg                                                                                                               <  8:236219/149‑1 (MQ=255)                                                  tGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGccaacc                                                                                                 >  6:60923/1‑148 (MQ=255)                                                   gAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGccaacca                                                                                                >  6:195988/1‑148 (MQ=255)                                                      gttCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACt                                                                                            >  6:90464/3‑149 (MQ=255)                                                        tCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTc                                                                                           >  2:43673/1‑148 (MQ=255)                                                        tCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTc                                                                                           >  8:252379/1‑148 (MQ=255)                                                          aaGGCCAGCCTGTTTGACCCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCCGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCg                                                                                          >  1:129732/1‑147 (MQ=255)                                                          aaGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGa                                                                                         <  5:195988/148‑1 (MQ=255)                                                          aaGGCCAGCCTGTCTGATCCCGTCCGGCCGCTCGACCACGACGCTCGGCGCCTGGAACTGGCCCAGCAGTACGGCAAGGTGGTGGACGCGCTGAACGCAGCC‑AAGCAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGaa                                                                                        <  6:164/149‑1 (MQ=255)                                                             gCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGt                                                                                     <  5:138587/149‑1 (MQ=255)                                                                 gCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGc                                                                                 <  5:60923/149‑1 (MQ=255)                                                                         gAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCt                                                                         >  3:165729/1‑149 (MQ=255)                                                                           aCCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGCACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTc                                                                        >  4:172981/1‑148 (MQ=255)                                                                            ccGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTc                                                                        >  3:13846/1‑147 (MQ=255)                                                                             cGGTCCGGACGCTCGACGTCGAGGCACGGCGCCTGGAACTGTCCCAGGAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGTTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAg                                                                     <  6:90306/149‑1 (MQ=255)                                                                               gTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCGAGCCCACCTCGAAGGTTAGCCGCTGCCTCa                                                                       >  8:211064/1‑145 (MQ=255)                                                                               gTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCa                                                                       <  7:211064/145‑1 (MQ=255)                                                                               gTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCt                                                                   >  8:206521/1‑149 (MQ=255)                                                                                  cGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGt                                                                 <  1:43673/148‑1 (MQ=255)                                                                                   gCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTAc                                                               <  3:294666/149‑1 (MQ=255)                                                                                       gCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGccaa                                                                                                   >  6:201418/1‑109 (MQ=255)                                                                                       gCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGccaa                                                                                                   <  5:201418/109‑1 (MQ=255)                                                                                         tCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTg                                                         >  1:312403/1‑149 (MQ=255)                                                                                                cGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTTGCCCAgg                                                  <  7:206521/149‑1 (MQ=255)                                                                                                   ggCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGt                                                                 <  6:204582/131‑1 (MQ=255)                                                                                                   ggCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGt                                                                 >  5:204582/1‑131 (MQ=255)                                                                                                      aCGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCTAAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATc                                             >  1:249052/1‑149 (MQ=255)                                                                                                           gcCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAg                                       >  3:88431/1‑149 (MQ=255)                                                                                                               ggAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCgtg                                   >  5:141565/1‑149 (MQ=255)                                                                                                                gAACTGGCCCAGCAGAACGGCAAGGTTGTGGCCGCGCCGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCgt                                    <  2:129732/147‑1 (MQ=255)                                                                                                                        ccAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCt                          <  4:165729/149‑1 (MQ=255)                                                                                                                             agAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCAc                     <  4:13846/149‑1 (MQ=255)                                                                                                                             agAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCAc                     <  5:90464/149‑1 (MQ=255)                                                                                                                                       ggtggtGGCCGCGCCGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTAc            >  2:54891/1‑148 (MQ=255)                                                                                                                                        tccttggCTGCGTTCAGCGCGGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTAc                  <  7:27213/137‑1 (MQ=255)                                                                                                                                        tccttggCTGCGTTCAGCGCGGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTAc                  >  8:27213/5‑141 (MQ=255)                                                                                                                                         tggtggCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAAc         >  7:9925/1‑149 (MQ=255)                                                                                                                                           ttggCTGCGTTCAGCGCGGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAACg        >  4:136751/2‑148 (MQ=255)                                                                                                                                           ttggCTGCGTTCAGCGCGGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAACg        >  2:97939/2‑148 (MQ=255)                                                                                                                                           gtggCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAACg        >  2:310520/1‑148 (MQ=255)                                                                                                                                             ggCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAACGCAc     >  5:71656/1‑149 (MQ=255)                                                                                                                                                cgcgCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCa                                >  4:11081/1‑119 (MQ=255)                                                                                                                                                cgcgCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCa                                <  3:11081/119‑1 (MQ=255)                                                                                                                                                cgcgCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAACGCACTGa  <  4:88431/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                      CCGGTACAGCAGAGCCTGGCCAGACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCC‑AAGGAGATCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAACGCACTGA  >  NC_002947/5645164‑5645455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑