Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,672,372 G→C 6.3% G208R (GGC→CGC)  PP_4979 → substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,672,3720GC 6.3% 202.2 / 4.3 64G208R (GGC→CGC) PP_4979substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (30/30);  new base C (2/2);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.32e-01

AGAAGCCAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGAGCAGCAGG  >  NC_002947/5672224‑5672519
                                                                                                                                                    |                                                                                                                                                   
agaagCCAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTgg                                                                                                                                                     <  7:90231/149‑1 (MQ=255)
    gCCAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCt                                                                                                                                                 >  3:264699/1‑149 (MQ=255)
         cTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGGCGATGAACTGCCGCTGGTAATCAGCCGCCCGGCGCAGGACCTGGGCCTGGTgc                                                                                                                                            >  6:172915/1‑149 (MQ=255)
          ttCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTgcg                                                                                                                                           >  4:163963/1‑149 (MQ=255)
            cGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTgcgc                                                                                                                                          <  3:310469/148‑1 (MQ=255)
                  gCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTg                                                                                                                                   >  8:43751/1‑149 (MQ=255)
                   cTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTgg                                                                                                                                  >  8:112641/1‑149 (MQ=255)
                        aCTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGcgcg                                                                                                                             >  8:200200/1‑149 (MQ=255)
                                gCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTg                                                                                                                     >  2:32304/1‑149 (MQ=255)
                                            tGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCttt                                                                                                         <  5:172915/149‑1 (MQ=255)
                                              aTCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGc                                                                                                       <  7:186041/149‑1 (MQ=255)
                                                  ccgaccgCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGaa                                                                                                   <  7:200200/149‑1 (MQ=255)
                                                    gaccgCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGaa                                                                                                   >  4:38038/1‑147 (MQ=255)
                                                         cAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGc                                                                                            <  7:33296/149‑1 (MQ=255)
                                                         cAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGc                                                                                            <  7:112641/149‑1 (MQ=255)
                                                             gTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCg                                                                                        <  4:37694/149‑1 (MQ=255)
                                                                  gCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAgg                                                                                                                          >  6:244752/1‑110 (MQ=255)
                                                                  gCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAgg                                                                                                                          <  5:244752/110‑1 (MQ=255)
                                                                  gCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTc                                                                                    >  5:291708/1‑148 (MQ=255)
                                                                    aTTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCTAg                                                                                 <  1:285111/149‑1 (MQ=255)
                                                                    aTTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAg                                                                                 <  1:285108/149‑1 (MQ=255)
                                                                    aTTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAg                                                                                 <  4:264699/149‑1 (MQ=255)
                                                                       aCCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCa                                                                               <  3:52327/148‑1 (MQ=255)
                                                                         cTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAaga                                                                                                 >  4:61484/1‑128 (MQ=255)
                                                                         cTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAaga                                                                                                 <  3:61484/128‑1 (MQ=255)
                                                                            cTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGc                                                                                                       >  4:250753/1‑119 (MQ=255)
                                                                            cTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGc                                                                                                       <  3:250753/119‑1 (MQ=255)
                                                                             tCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCg                                                                        >  1:259162/1‑149 (MQ=255)
                                                                                  cAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTa                                                                   >  7:68012/1‑149 (MQ=255)
                                                                                        ggCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAg                                                              <  8:68012/148‑1 (MQ=255)
                                                                                          ccTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGa                                                           >  3:157088/1‑149 (MQ=255)
                                                                                              cAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAg                                                       >  6:155892/1‑149 (MQ=255)
                                                                                              cAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAg                                                       <  2:259162/149‑1 (MQ=255)
                                                                                               aaCAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGcc                                                                                                      >  4:294276/1‑101 (MQ=255)
                                                                                               aaCAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGcc                                                                                                      <  3:294276/101‑1 (MQ=255)
                                                                                                       gTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTta                                              <  3:38038/149‑1 (MQ=255)
                                                                                                       gTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTta                                              <  5:106188/149‑1 (MQ=255)
                                                                                                             gaaCTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGAAGCGATCAGCAACCGTTATACAAc                                        <  7:43751/149‑1 (MQ=255)
                                                                                                              aaCTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCg                                                                                        >  2:165118/1‑100 (MQ=255)
                                                                                                              aaCTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCg                                                                                        <  1:165118/100‑1 (MQ=255)
                                                                                                              aaCTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAAcc                                       <  1:52957/149‑1 (MQ=255)
                                                                                                              aaCTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAAcc                                       >  6:269102/1‑149 (MQ=255)
                                                                                                              aaCTGCCGCTGGT‑ATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAAcc                                       >  6:61726/1‑148 (MQ=255)
                                                                                                                  gCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGa                                                                                                                            <  4:71858/60‑1 (MQ=255)
                                                                                                                  gCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGa                                                                                                                            >  3:71858/1‑60 (MQ=255)
                                                                                                                  gCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCGTTGGCGAAGAGCTGCAGCGGTTCAAGTAGGAGCCGGCCGATGCGGCGATCAGCACCCGTGATACACCCGaca                                   >  6:228454/1‑149 (MQ=255)
                                                                                                                       tGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGCTGCGCAATCCAGGGCGCGAGGCACTGGACGTGGACTTTGCCGAAGAGCTGCAGCGGTTCAAGAAGGAGCAGTCCTATCAAGCGAACAGCAACCGTTAAACAACCGacaaagg                               >  6:322303/1‑144 (MQ=255)
                                                                                                                           aaTCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAAcc                                       <  3:299832/136‑1 (MQ=255)
                                                                                                                           aaTCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAAcc                                       >  4:299832/1‑136 (MQ=255)
                                                                                                                             tCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACAtt                        <  3:163963/149‑1 (MQ=255)
                                                                                                                               aGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATtct                      >  2:264943/1‑149 (MQ=255)
                                                                                                                               aGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATtc                       <  5:155892/148‑1 (MQ=255)
                                                                                                                                gccgccAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTtat                                             <  3:174075/125‑1 (MQ=255)
                                                                                                                                gccgccAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGaa                             <  5:156609/141‑1 (MQ=255)
                                                                                                                                gccgccAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGaa                             >  6:156609/1‑141 (MQ=255)
                                                                                                                                gccgccAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGAGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTtat                                             >  4:174075/1‑125 (MQ=255)
                                                                                                                                   gccAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAAc                  <  4:157088/149‑1 (MQ=255)
                                                                                                                                   accAGGCCCAGGTACTGCGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCa                                                        <  2:108988/110‑1 (MQ=255)
                                                                                                                                   accAGGCCCAGGTACTGCGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAAc                  >  7:94475/2‑149 (MQ=255)
                                                                                                                                   accAGGCCCAGGTACTGCGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAAc                  >  6:194961/2‑149 (MQ=255)
                                                                                                                                       ggCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCg              >  5:86776/1‑149 (MQ=255)
                                                                                                                                          gcAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGtt            >  5:183893/1‑148 (MQ=255)
                                                                                                                                           cAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGa          <  6:291708/149‑1 (MQ=255)
                                                                                                                                            aggtacTGCGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGag         <  8:94475/144‑1 (MQ=255)
                                                                                                                                            aGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGag         >  3:35337/1‑149 (MQ=255)
                                                                                                                                                   gggCCTGGTGCGCAAGCCTGGGCGCGAGGCAGTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGag         <  4:536/142‑1 (MQ=255)
                                                                                                                                                   gggCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAAc                  >  7:3055/1‑133 (MQ=255)
                                                                                                                                                   gggCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAAc                  <  8:3055/133‑1 (MQ=255)
                                                                                                                                                   gggCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGagcagaacg  >  3:536/1‑145 (MQ=255)
                                                                                                                                                    ggCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACATTGGAAACATTCTCAACGCCGTTGAGcagcag   <  1:32304/147‑1 (MQ=255)
                                                                                                                                                    ggCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGAGcagcag   <  5:61726/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGAAGCCAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGAGCAGCAGG  >  NC_002947/5672224‑5672519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑