Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,674,134 C→T 5.6% R459R (CGC→CGT)  rhlE‑II → ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,674,1340CT 5.6% 219.6 / 2.7 71R459R (CGC→CGT) rhlE‑IIATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base C (34/33);  new base T (2/2);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GCGAAGGTGGTAAAGACACCAACGGCGGCCGCAAGGACAAAGGCAAGGACAAAGGCAAAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCGGTAACCGCGCGGACTATGTCAGCCCTTACCAGGGCAAGAACCAGGGCCGC  >  NC_002947/5673986‑5674272
                                                                                                                                                    |                                                                                                                                          
gCGAAGGTGGTAAAGACACCAACAGCGGCCGCAAGGACAAAGGCAAGGACAAAGGCAAAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGc                                                                                                                                            <  7:44981/149‑1 (MQ=255)
  gAAGGTGGTAAAGACACCAACGGCGGCCGCAAGGACAAAGGCAAGGACAAAGGCAAAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGccc                                                                                                                                          >  8:134887/1‑149 (MQ=255)
  gAAGGTGGTAAAGACACCAACGGCGGCCGCAAGGACAAAGGCAAGGACAAAGGCAAAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGccc                                                                                                                                          <  4:213274/149‑1 (MQ=255)
         gtAAAGACACCAACGGCGGCCGCAAGGACAAAGGCAAGGACAAAGGCAAAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGcagcag                                                                                                                                   <  4:112260/149‑1 (MQ=255)
           aaaGACACCAACGGCGGCCGCAAGGACAAAGGCAAGGACAAAGGCAAAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGTCCGCAGCAGGc                                                                                                                                 >  8:213225/1‑149 (MQ=255)
           aaaGACACCAACGGCGGCCGCAAGGACAAAGGCAAGGACAAAGGCAAAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGTCCGCAGCAGGc                                                                                                                                 >  8:209090/1‑149 (MQ=255)
            aaGACACCAACGGCGGCCGCAAGGACAAAGGCAAGGACAAAGGCAAAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGc                                                                                                                                 <  2:300794/148‑1 (MQ=255)
                cacCAACGGCGGCCGCAAGGACAAAGGCAAGGACAAAGGCAAAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGc                                                                                                                             <  7:104376/148‑1 (MQ=255)
                cacCAACGGCGGCCGCAAGGACAAAGGCAAGGACAAAGGCAAAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCa                                                                                                                            <  3:49481/149‑1 (MQ=255)
                     aCGGCGGCCGCAAGGACAAAGGCAAGGACAAAGGCAAAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGcagcag                                                                                                                                   <  6:287434/137‑1 (MQ=255)
                     aCGGCGGCCGCAAGGACAAAGGCAAGGACAAAGGCAAAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGcagcag                                                                                                                                   >  5:287434/1‑137 (MQ=255)
                      cggcggCCGCAAGGACAAAGGCAAGGACAAAGGCAAAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACa                                                                                                                      <  3:217976/149‑1 (MQ=255)
                        gcggcCGCAAGGACAAAGGCAAGGACAAAGGCAAAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGc                                                                                                                    <  2:256987/149‑1 (MQ=255)
                        gcggcCGCAAGGACAAAGGCAAGGACAAAGGCAAAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGc                                                                                                                    <  8:26454/149‑1 (MQ=255)
                            ccGCAAGGACAAAGGCAAGGACAAAGGCAAAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGc                                                                                                                <  2:228218/149‑1 (MQ=255)
                                  ggacaaaggcaaggacaaaggcaaAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAgc                                                                                                          <  7:134887/149‑1 (MQ=255)
                                  ggacaaaggcaaggacaaaggcaaAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAgc                                                                                                          <  5:258800/149‑1 (MQ=255)
                                    acaaaggcaaggacaaaggcaaAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAgccg                                                                                                        >  7:285717/1‑149 (MQ=255)
                                      aaaggcaaggacaaaggcaaAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAgccgcc                                                                                                      >  7:181628/1‑149 (MQ=255)
                                      aaaggcaaggacaaaggcaaAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAgccgcc                                                                                                      <  4:269435/149‑1 (MQ=255)
                                            aaggacaaaggcaaAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACa                                                                                                                      <  7:114919/127‑1 (MQ=255)
                                            aaggacaaaggcaaAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACa                                                                                                                      >  8:114919/1‑127 (MQ=255)
                                                 caaaggcaaAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGCCAAGAAGTCACGCCCGCAGCAGGCCAGCAAGACCAGCGTGCCGAAGCCGCCGGCAGACcgcg                                                                                           >  4:298616/1‑149 (MQ=255)
                                                     ggcaaAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGAccc                                                                                       >  3:8585/1‑149 (MQ=255)
                                                        aaaGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGaa                                                                                                            >  6:315788/1‑125 (MQ=255)
                                                        aaaGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGaa                                                                                                            <  5:315788/125‑1 (MQ=255)
                                                        aaaGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCgg                                                                                     >  2:117198/1‑148 (MQ=255)
                                                         aaGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGaa                                                                                   <  6:246253/149‑1 (MQ=255)
                                                          aGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCgg                                                                                     >  3:7327/1‑146 (MQ=255)
                                                            ccAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGaa                                                                                                            >  2:158462/1‑121 (MQ=255)
                                                            ccAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGaa                                                                                                            <  1:158462/121‑1 (MQ=255)
                                                                gcagcaAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGaa                                                                                   >  8:8777/1‑142 (MQ=255)
                                                                gcagcaAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGaa                                                                                   <  7:8777/142‑1 (MQ=255)
                                                                 cagcaAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGTCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCt                                                                           <  7:213225/149‑1 (MQ=255)
                                                                 cagcaAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGTCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCt                                                                           <  7:209090/149‑1 (MQ=255)
                                                                  agcaAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTg                                                                          >  7:208308/1‑149 (MQ=255)
                                                                      aGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGaa                                                                                                            <  4:183751/111‑1 (MQ=255)
                                                                      aGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGaa                                                                                                            >  3:183751/1‑111 (MQ=255)
                                                                        cTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGgacgac                                                                    <  4:8585/149‑1 (MQ=255)
                                                                             gCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCt                                                                           <  7:177619/137‑1 (MQ=255)
                                                                             gCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCt                                                                           >  8:177619/1‑137 (MQ=255)
                                                                                        cgacAAGGAAAAGAGTGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCg                                                    >  6:77061/1‑149 (MQ=255)
                                                                                            aaGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCGGTaa                                                >  1:269108/1‑149 (MQ=255)
                                                                                              ggAAAAGAGCGGCGCCAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCg                                                    >  2:63998/1‑143 (MQ=255)
                                                                                              ggAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCg                                                    <  1:63998/143‑1 (MQ=255)
                                                                                               gAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCGGTAACcg                                             >  6:111434/1‑149 (MQ=255)
                                                                                                  aaGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGga                                                                        >  2:108270/1‑119 (MQ=255)
                                                                                                       cggcgACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCGGTAACCGCGCGGAc                                      >  3:235285/1‑148 (MQ=255)
                                                                                                        ggcgACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCGGTAACCGCGCGGACTa                                    <  8:208308/149‑1 (MQ=255)
                                                                                                         gcgACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCg                                                             <  2:91087/123‑1 (MQ=255)
                                                                                                         gcgACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCg                                                             >  1:91087/1‑123 (MQ=255)
                                                                                                          cgACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAACAGTTCCTGGACGACGAACTCGATAACTTCGGTAACCGCGCGGACTATg                                  <  4:7327/149‑1 (MQ=255)
                                                                                                              aaGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCTATAACTTCGGTAACCGCGCGGACTATGTCAg                              >  3:62507/1‑149 (MQ=255)
                                                                                                                gcagcaCCCGCGCAAGCCGCGTGACACGACGTCACGCCCGCAGCAGGCCAGCAATAACCGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGGTCCTGGACGACGAACTCGATAACTTCGGTAACCGCGCGGACTATGTCAGcc                            <  8:273075/149‑1 (MQ=255)
                                                                                                                gcagcaACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCGGTAACCGCGCGGACTATGTCAGcc                            <  2:269108/149‑1 (MQ=255)
                                                                                                                 cagcaACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCGGTAACCGCGCGGACTATGTCAGccc                           >  7:270956/1‑149 (MQ=255)
                                                                                                                  agcaACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCGGTAACCGCGCGGACTATGTCAGccc                           <  1:117198/148‑1 (MQ=255)
                                                                                                                     aaCCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCGGTAACCGCGCGGACTATGTCAGCCCTTAc                       <  5:111434/149‑1 (MQ=255)
                                                                                                                     aaCCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCGGTAACCGCGCGGACTATGTCAGCCCTTAc                       >  5:270266/1‑149 (MQ=255)
                                                                                                                     aaCCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCGGTAACCGCGCGGACTATGTCAGCCCTTAc                       >  2:218772/1‑149 (MQ=255)
                                                                                                                     aaCCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCGGTAACCGCGCGGACTATGTCAGCCCTTAc                       >  3:116063/1‑149 (MQ=255)
                                                                                                                     aaCCGCGCAAGCAGCGTGACAAGAAGTCACGCACGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCAGGAAGAGTTCCTCGACGACGAACTCGATAACTTCGGTAACCGCGCGGACTATGACAGCCCTTAc                       >  6:1257/1‑149 (MQ=255)
                                                                                                                       ccGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCGGTAAcc                                              <  2:167395/124‑1 (MQ=255)
                                                                                                                       ccGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCGGTAAcc                                              >  1:167395/1‑124 (MQ=255)
                                                                                                                           gcAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCGGTAACcgcgc                                          >  7:33707/1‑124 (MQ=255)
                                                                                                                           gcAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCGGTAACcgcgc                                          <  8:33707/124‑1 (MQ=255)
                                                                                                                                     tGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCGGTAACCGCGCGGACTATGTCAGCCCTTACCAGGGCAAGAACCAgg       <  5:77061/149‑1 (MQ=255)
                                                                                                                                         aagaagTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGCCCGCGACCCGGAAGAGTTCCTGGACCACGAACTCGATAACTTCGGTAACCGCGCGGACTATGTCAGCCCTTACCAGGGCAAGAACCAGGGCCg   >  1:273857/1‑149 (MQ=255)
                                                                                                                                         aagaagTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCGGTAACCGCGCGGACTATGTCa                               <  4:242345/121‑1 (MQ=255)
                                                                                                                                         aagaagTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCGGTAACCGCGCGGACTATGTCa                               >  3:242345/1‑121 (MQ=255)
                                                                                                                                         aagaagTCACGCCCGCAGCAGGCCAGCAATAAAAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCGGTAACCGCGCGGACTATGTCAGCCCTTACCAGGGCAAGAACCAGGGCCg   >  8:5649/1‑149 (MQ=255)
                                                                                                                                           gaagTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCGGTAACCGCGCGGACTATGTCAGCCCTTACCAGGGCAAGAACCAGGGCCGc  >  6:325942/1‑148 (MQ=255)
                                                                                                                                            aagTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGgacgac                                                                    <  2:26888/81‑1 (MQ=255)
                                                                                                                                            aagTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGgacgac                                                                    >  1:26888/1‑81 (MQ=255)
                                                                                                                                                    |                                                                                                                                          
GCGAAGGTGGTAAAGACACCAACGGCGGCCGCAAGGACAAAGGCAAGGACAAAGGCAAAGCCAGGCAGCAAGCTGCGGCCAAGCCGGACGACAAGGAAAAGAGCGGCGACAAGCAGCAACCGCGCAAGCCGCGTGACAAGAAGTCACGCCCGCAGCAGGCCAGCAATAACAGCGTGCCGAAGCCGCCGGCAGACCGCGACCCGGAAGAGTTCCTGGACGACGAACTCGATAACTTCGGTAACCGCGCGGACTATGTCAGCCCTTACCAGGGCAAGAACCAGGGCCGC  >  NC_002947/5673986‑5674272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑