Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,679,147 C→T 9.2% A258V (GCC→GTC)  bioA → adenosylmethionine‑‑8‑amino‑7‑oxononanoate aminotransferase BioA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,679,1470CT 9.2% 181.5 / 7.1 65A258V (GCC→GTC) bioAadenosylmethionine‑‑8‑amino‑7‑oxononanoate aminotransferase BioA
Reads supporting (aligned to +/- strand):  ref base C (30/29);  new base T (4/2);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

GAACACCACGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTACCAGGCGTTCT  >  NC_002947/5679002‑5679293
                                                                                                                                                  |                                                                                                                                                  
gAACACCACGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGt                                                                                                                                                 >  2:207723/1‑149 (MQ=255)
  acacCACGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTgg                                                                                                                                               <  7:176087/149‑1 (MQ=255)
  acacCACGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTgg                                                                                                                                               <  1:95053/149‑1 (MQ=255)
     ccacGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCCCGGCGATCTCGTCGTggata                                                                                                                                            <  4:208649/149‑4 (MQ=255)
              gATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCg                                                                                                                                     <  6:24878/147‑1 (MQ=255)
               aTCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGGCGATCTCGTCGTggata                                                                                                                                            <  3:39447/139‑4 (MQ=255)
               aTCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGGCGATCTCGTCGTggata                                                                                                                                            >  4:39447/1‑136 (MQ=255)
                       cGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGa                                                                                                                          <  5:123348/149‑1 (MQ=255)
                          gATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGt                                                                                                                       >  4:77805/1‑149 (MQ=255)
                           aTCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGt                                                                                                                       <  5:237282/148‑1 (MQ=255)
                                cgcgCCGCTGATACCGGGTGCCGGTGGCAGGCGCATGTACCACCCGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGCCGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCt                                                                                                                 <  7:131448/146‑1 (MQ=255)
                                  aGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTGACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTg                                                                                                                >  1:233657/1‑149 (MQ=255)
                                  aGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGc                                                                                                               <  1:207723/149‑1 (MQ=255)
                                            aCAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCg                                                                                                     >  1:283641/1‑149 (MQ=255)
                                                                 cATGTACCACCCGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCtgt                                                                                <  2:233657/149‑1 (MQ=255)
                                                                  ctGTACCACCCTGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGAttt                                                                                     <  6:322100/142‑1 (MQ=255)
                                                                  aTGTACCACACGGTGT‑ACCTCAAGTTGTTGCTCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGAGAGCAAGCCGGCATCCGCCCGGAttt                                                                                     >  5:322100/1‑143 (MQ=255)
                                                                    gTACCACCCGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGc                                                                                                       >  8:282155/1‑123 (MQ=255)
                                                                    gTACCACCCGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGc                                                                                                       <  7:282155/123‑1 (MQ=255)
                                                                       ccaccCGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCt                                                                                  <  2:306637/141‑1 (MQ=255)
                                                                       ccaccCGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCt                                                                                  >  1:306637/1‑141 (MQ=255)
                                                                        caccCGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTc                                                                         >  5:292902/1‑149 (MQ=255)
                                                                        caccCGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTc                                                                         >  5:324228/1‑149 (MQ=255)
                                                                          cccGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCtgt                                                                                >  5:180367/1‑140 (MQ=255)
                                                                          cccGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCtgt                                                                                <  6:180367/140‑1 (MQ=255)
                                                                                   aCCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGccc                                                                                           <  1:139851/121‑1 (MQ=255)
                                                                                   aCCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGccc                                                                                           >  2:139851/1‑121 (MQ=255)
                                                                                    ccTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGa                                                              >  4:236820/1‑149 (MQ=255)
                                                                                     cTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGAc                                                             >  3:255575/1‑149 (MQ=255)
                                                                                      tCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGAcc                                                            >  3:160294/1‑149 (MQ=255)
                                                                                       cAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGc                                                                              >  8:183248/1‑130 (MQ=255)
                                                                                       cAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGc                                                                              <  7:183248/130‑1 (MQ=255)
                                                                                           ttgttgCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACcggcgg                                                       <  6:324228/149‑1 (MQ=255)
                                                                                             gttgCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCACCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCggcggc                                                      <  4:282250/148‑1 (MQ=255)
                                                                                                  gcgAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTg                                                <  3:236820/149‑1 (MQ=255)
                                                                                                   cgAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCTTCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGc                                               >  5:126462/1‑149 (MQ=255)
                                                                                                     aGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCg                                             <  6:292902/149‑1 (MQ=255)
                                                                                                         cTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCg                                                                                                              >  4:139261/1‑80 (MQ=255)
                                                                                                         cTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCg                                                                                                              <  3:139261/80‑1 (MQ=255)
                                                                                                         cTGCGACCGCTATGACGTGCACCATATCCACCACGACATCGCCCTCAGCTTCGGCCGCACCCGCACCAACTTCGCCTGCGAGCAAGCCGGCATCCGCCCCTAAATCCTGTGCCAGTCCGAGGCCCTGACCGGAGGCTACCTGCCGCAgg                                         >  6:82840/1‑149 (MQ=255)
                                                                                                          tGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGgc                                        >  7:88062/1‑149 (MQ=255)
                                                                                                               ccGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCtgt                                                                                >  2:129803/1‑104 (MQ=255)
                                                                                                               ccGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCtgt                                                                                <  1:129803/104‑1 (MQ=255)
                                                                                                                cGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCgcctg                                  >  2:206891/1‑149 (MQ=255)
                                                                                                                  cTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGACCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCgcctgcc                                >  3:162412/1‑149 (MQ=255)
                                                                                                                   tATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCgcctgcct                               >  4:44107/1‑149 (MQ=255)
                                                                                                                     tGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGa                             >  7:97910/1‑149 (MQ=255)
                                                                                                                         gTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGCCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGaccac                         <  4:162412/149‑1 (MQ=255)
                                                                                                                           gCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGaccacc                        >  6:233095/1‑148 (MQ=255)
                                                                                                                               gaagcccACGGCGATCTCGTCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGAc                     >  2:308403/6‑147 (MQ=255)
                                                                                                                                  aTCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCGAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAg                  <  5:82840/147‑1 (MQ=255)
                                                                                                                                    ccACGGCGATCTCGTCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGgtg               >  1:50337/1‑148 (MQ=255)
                                                                                                                                    ccACGGCGATCTCGTCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCGGCGAGCAAGCCGGTATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGgtg               >  6:259988/1‑148 (MQ=255)
                                                                                                                                    ccACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCt                                                 <  2:99910/114‑1 (MQ=255)
                                                                                                                                    ccACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCt                                                 >  1:99910/1‑114 (MQ=255)
                                                                                                                                    ccACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTACCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGgtg               >  1:99971/1‑148 (MQ=255)
                                                                                                                                     cACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGgtgt              <  3:77805/148‑1 (MQ=255)
                                                                                                                                      acgacgAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCTTCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTAc            <  6:126462/149‑1 (MQ=255)
                                                                                                                                      acgacgAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGaccac                         <  3:151328/136‑1 (MQ=255)
                                                                                                                                      acgacgAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGaccac                         >  4:151328/1‑136 (MQ=255)
                                                                                                                                        gacgaGATCGCCGTGGGCTTCGGCCGCAGCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAg                  >  1:177487/1‑141 (MQ=255)
                                                                                                                                        gacgaGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAg                  <  2:177487/141‑1 (MQ=255)
                                                                                                                                               tCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTACCAGGCGTTc   >  6:274910/1‑149 (MQ=255)
                                                                                                                                               tCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTACCAGGCGTTc   <  3:44107/149‑1 (MQ=255)
                                                                                                                                                 gCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCAGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTACCAGGCGTTCt  <  4:160294/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GAACACCACGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGT‑ACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTACCAGGCGTTCT  >  NC_002947/5679002‑5679293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑