Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,683,673 A→G 8.0% F1074L (TTC→CTC)  PP_4988 ← signal transduction histidine kinase CheA/CheY‑like receiver

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,683,6730AG 8.0% 139.9 / 3.0 50F1074L (TTC→CTC) PP_4988signal transduction histidine kinase CheA/CheY‑like receiver
Reads supporting (aligned to +/- strand):  ref base A (23/23);  new base G (2/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-01

GCGGTCCATTTCCAGCGGGTCGAAGTCGTCATAGGCATCGCCTTCGAACTGCTGCCGGCTGGAGATTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTG  >  NC_002947/5683527‑5683821
                                                                                                                                                  |                                                                                                                                                     
gCGGTCCATTTCCAGCGGGTCTAAGTCGTCATAGGCATCGCCTTCGAACAGCTGCCGGCTGGAGATTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGGCTCCATCTCGTTGAGGGCGAACt                                                                                                                                                     <  4:275810/149‑1 (MQ=255)
       aTTTCCAGCGGGTCGAAGTCGTCATAGGCATCGCCTTCGAACTGCTGCCGGCTGGAGATTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGcgtc                                                                                                                                              <  6:101096/149‑1 (MQ=255)
         ttCCAGCGGGTCGAAGTCGTCATAGGCATCGCCTTCGAACTGCTGCCGGCTGGAGATTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGcgtcgt                                                                                                                                            >  1:42613/1‑149 (MQ=255)
            cAGCGGGTCGAAGTCGTCATAGGCATCGCCTTCGAACTGCTGCCGGCTGGAGATTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTg                                                                                                                                          <  7:183090/148‑1 (MQ=255)
              gCGGGTCGAAGTCGTCATAGGCATCGCCTTCGAACTGCTGCCGGCTGGAGATTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGAcc                                                                                                                                       <  3:15374/149‑1 (MQ=255)
                     gAAGTCGTCATAGGCATCGCCTTCGAACTGCTGCCGGCTGGAGATTCGCCCCTGGGTCTCGACGTCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCt                                                                                                                                >  2:63413/1‑149 (MQ=255)
                     gAAGTCGTCATAGGCATCGCCTTCGAACTGCTGCCGGCTGGAGATTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCt                                                                                                                                >  2:66109/1‑149 (MQ=255)
                         tcgtcATAGGCATCGCCTTCGAACTGCTGCCGGCTGGAGATTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGAt                                                                                                                            <  6:325929/149‑1 (MQ=255)
                            tcATAGGCATCGCCTTCGAACTGCTGCCGGCTGGAGATTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATgcg                                                                                                                         >  5:80834/1‑149 (MQ=255)
                                         cTTCGAACTGCTGCCGGCTGGAGATTCGCCCCTGGGTCTCGACGTCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATc                                                                                                            <  1:63413/149‑1 (MQ=255)
                                         cTTCGAACTGCTGCCGGCTGGAGATTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGgatg                                                                                                               >  8:18371/1‑146 (MQ=255)
                                         cTTCGAACTGCTGCCGGCTGGAGATTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGgatg                                                                                                               <  7:18371/146‑1 (MQ=255)
                                            cGAACTGCTGCCGGCTGGAGATTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGAcc                                                                                                                                       <  2:204591/119‑1 (MQ=255)
                                            cGAACTGCTGCCGGCTGGAGATTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGAcc                                                                                                                                       >  1:204591/1‑119 (MQ=255)
                                            cGAACTGCTGCCGGCTGGAGATTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGa                                                                                                          <  2:201532/148‑1 (MQ=255)
                                            cGAACTGCTGCCGGCTGGAGATTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGa                                                                                                          <  7:125081/148‑1 (MQ=255)
                                              aaCTGCTGCCGGCTGGTGATTGGGCCGTGGGTCTCGACATCCAGACGCAGCAACTGATCACTCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTg                                                                                                      <  2:42613/149‑1 (MQ=255)
                                              aaCTGCTGCCGGCTGGAGATTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTg                                                                                                      <  8:19108/149‑1 (MQ=255)
                                              aaCTGCTGCCGGCTGGAGATTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACGGATCACGCATGCGCTCGAGTGTGGTCTCCCTCTCGTTGAGGGCGCACTGCGCGTCGTTGACCTGTTGCTCGCTGCGCCGCCGGATGATCGA‑GTg                                                                                                      >  5:233085/1‑149 (MQ=255)
                                               aCTGCTGCCGGCTGGAGATTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGc                                                                                                     >  7:139036/1‑149 (MQ=255)
                                                     gcCGGCTGGAGATTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGa                                                                                                          <  7:187389/139‑1 (MQ=255)
                                                     gcCGGCTGGAGATTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGa                                                                                                          >  8:187389/1‑139 (MQ=255)
                                                           tGGAGATTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAgg                                                                                         >  4:178872/1‑149 (MQ=255)
                                                              agaTTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGtt                                                                                                                                   <  6:138438/105‑1 (MQ=255)
                                                              agaTTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGtt                                                                                                                                   >  5:138438/1‑105 (MQ=255)
                                                               gaTTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCGCGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTAc                                                                                     >  6:313623/1‑149 (MQ=255)
                                                                    gCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTATGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAgg                                                                                >  4:81280/1‑149 (MQ=255)
                                                                     ccccTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCATGTTCACCAgg                                                                                >  8:9858/1‑148 (MQ=255)
                                                                      cccTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAGgtc                                                                              <  8:139036/149‑1 (MQ=255)
                                                                           ggTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAGGTCGTCGa                                                                         <  6:80834/149‑1 (MQ=255)
                                                                                        aGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGACGTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGt                                                             <  1:66109/149‑1 (MQ=255)
                                                                                        aGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAGgtcgtc                                                                           >  3:306902/1‑134 (MQ=255)
                                                                                        aGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAGgtcgtc                                                                           <  4:306902/134‑1 (MQ=255)
                                                                                        aGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTc                                                            <  3:178872/149‑1 (MQ=255)
                                                                                              agcaACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCtt                                                      >  3:150992/1‑149 (MQ=255)
                                                                                                   cTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCt                                                                                                                                <  1:79321/71‑1 (MQ=255)
                                                                                                   cTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCt                                                                                                                                >  2:79321/1‑71 (MQ=255)
                                                                                                   cTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGAcc                                                  >  8:129164/1‑148 (MQ=255)
                                                                                                          cGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCAcc                                          >  6:163053/1‑149 (MQ=255)
                                                                                                             aTGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAGCTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCgg                                       >  3:113100/1‑149 (MQ=255)
                                                                                                             aTGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAGCTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCgg                                       >  3:117763/1‑149 (MQ=255)
                                                                                                                     gAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCg                                 <  7:129164/147‑1 (MQ=255)
                                                                                                                       gtgtGGTCTCCATCTCGTTGAGGGCGAGCTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGctct                             <  4:113100/149‑1 (MQ=255)
                                                                                                                       gtgtGGTCTCCATCTCGTTGAGGGCGAGCTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGctct                             <  4:117763/149‑1 (MQ=255)
                                                                                                                          tgGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGgc                          >  6:311884/1‑149 (MQ=255)
                                                                                                                             tctcCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCgct                       >  5:316535/1‑149 (MQ=255)
                                                                                                                                    ctcGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCg                >  3:28399/1‑149 (MQ=255)
                                                                                                                                        ttGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGAcc                                                        >  3:228301/1‑105 (MQ=255)
                                                                                                                                        ttGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGAcc                                                        <  4:228301/105‑1 (MQ=255)
                                                                                                                                            gggCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTc         <  7:9858/148‑1 (MQ=255)
                                                                                                                                                  aCTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTg  <  4:28399/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GCGGTCCATTTCCAGCGGGTCGAAGTCGTCATAGGCATCGCCTTCGAACTGCTGCCGGCTGGAGATTCGCCCCTGGGTCTCGACATCCAGACGCAGCAACTGATCACGCATGCGCTCGAGTGTGGTCTCCATCTCGTTGAGGGCGAACTGCGCGTCGTTGACCTGTTGCTCGATGCGCCCCCGGATGATCGA‑GTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTG  >  NC_002947/5683527‑5683821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑