Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,707,113	G→C	6.1%	L377L (CTG→CTC) ‡	PP_5009 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,707,113	0	G	C	6.1%	212.3 / 3.6	66	L377L (CTG→CTC) ‡	PP_5009	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (27/35);  new base C (2/2);  total (29/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.53e-01

GACCTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGCACACAAGGG  >  NC_002947/5706967‑5707257                                                                                                                                                    |                                                                                                                                                   gACCTGCCTTCGCAGGAACGAACTGCGTCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTgg                                                                                                                                                   <  7:22654/148‑1 (MQ=255)    cTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGa                                                                                                                                               >  6:223388/1‑149 (MQ=255)       ccTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCATCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGAtat                                                                                                                                            >  4:200287/1‑147 (MQ=255)       ccTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGAt                                                                                                                                              <  5:223388/147‑1 (MQ=255)       ccTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGAt                                                                                                                                              <  7:131932/147‑1 (MQ=255)         ttCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTc                                                                                                                                           >  2:273839/1‑148 (MQ=255)            gCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATg                                                                                                                                             <  3:284819/143‑1 (MQ=255)            gCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATg                                                                                                                                             >  4:284819/1‑143 (MQ=255)            gCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCg                                                                                                                                          >  4:121110/1‑146 (MQ=255)            gCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGaa                                                                                                                                        <  8:112433/148‑1 (MQ=255)                gaacgaacTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGGTCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGatat                                                                                                                                   >  2:245415/1‑149 (MQ=255)                    gaacTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGt                                                                                                                               <  3:121110/149‑1 (MQ=255)                       cTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGAt                                                                                                                            <  7:244309/149‑1 (MQ=255)                        tGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGaa                                                                                                                                        >  7:98498/1‑136 (MQ=255)                        tGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGaa                                                                                                                                        <  8:98498/136‑1 (MQ=255)                        tGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGAt                                                                                                                            >  6:133701/1‑148 (MQ=255)                        tGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCAATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCGGGGCGATGTCGAAGATATCCGTGAt                                                                                                                            >  5:143703/1‑148 (MQ=255)                          cgcCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑a                                                                                                                         <  4:199186/148‑1 (MQ=255)                           gcCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑Acg                                                                                                                       >  4:309201/1‑149 (MQ=255)                                   tCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑Acgccgctc                                                                                                                 <  5:133701/147‑1 (MQ=255)                                    cAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGt                                                                                                                               >  3:548/1‑133 (MQ=255)                                    cAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGt                                                                                                                               <  4:548/133‑1 (MQ=255)                                     aGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTTCTCGGTCTGGCGGCGAACCAAGGTCGGTCTAAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑Acgccgctcgcc                                                                                                              <  1:245415/149‑1 (MQ=255)                                     aGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑Acgccgctcgccg                                                                                                             >  4:38411/1‑149 (MQ=255)                                            cgcgATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGt                                                                                                      <  2:41544/149‑1 (MQ=255)                                            cgcgATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGt                                                                                                      <  5:102774/149‑1 (MQ=255)                                                aTGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTgaga                                                                                                  <  6:179848/149‑1 (MQ=255)                                                 tGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGAc                                                                                                 >  5:304566/1‑149 (MQ=255)                                                     tgctCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTga                                                                                                    <  3:46889/142‑1 (MQ=255)                                                     tgctCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTga                                                                                                    >  4:46889/1‑142 (MQ=255)                                                                    cTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATc                                                                               >  7:124427/1‑147 (MQ=255)                                                                     tGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTAt                                                                                <  3:159205/145‑1 (MQ=255)                                                                     tGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTAt                                                                                >  4:159205/1‑145 (MQ=255)                                                                         gACCGCTACGCCTATCACACGTCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGTCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACaa                                                                        <  5:218613/149‑1 (MQ=255)                                                                         gACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACaa                                                                        <  3:199898/149‑1 (MQ=255)                                                                           ccGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAgg                                                                      <  8:63060/149‑1 (MQ=255)                                                                            cGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATcc                                                                                                                                 <  8:165736/91‑1 (MQ=255)                                                                            cGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATcc                                                                                                                                 >  7:165736/1‑91 (MQ=255)                                                                            cGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAgg                                                                      >  1:274827/1‑148 (MQ=255)                                                                            cGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAgg                                                                      >  2:184112/1‑148 (MQ=255)                                                                             gCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCa                                                                    <  3:309201/149‑1 (MQ=255)                                                                              cTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTA‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAg                                                                   >  7:215717/1‑149 (MQ=255)                                                                                 cGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAGTac                                                                >  2:84966/1‑149 (MQ=255)                                                                                  gCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCTCGCAGACCAGCTATCCAGACAAGGCAGTac                                                                <  1:184112/149‑1 (MQ=255)                                                                                       tCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAGTACACGAcc                                                          <  6:304566/149‑1 (MQ=255)                                                                                         acacGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAGTACACGAccc                                                         >  8:207156/1‑148 (MQ=255)                                                                                               cACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGAt                                                  <  8:30102/149‑1 (MQ=255)                                                                                                    cTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGa                                                                           <  6:92562/119‑1 (MQ=255)                                                                                                    cTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGa                                                                           >  5:92562/1‑119 (MQ=255)                                                                                                    cTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGc                                              <  7:207156/148‑1 (MQ=255)                                                                                                    cTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGc                                              <  3:38411/148‑1 (MQ=255)                                                                                                             gcggcgAACCACGGGCGGTC‑GAAGTGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGc                                      <  8:215717/147‑1 (MQ=255)                                                                                                                     ccAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTAACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTccc                               >  1:86944/1‑147 (MQ=255)                                                                                                                          gtcggtc‑gAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCcat                        >  7:177423/1‑148 (MQ=255)                                                                                                                            cggtc‑gAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCcatcat                     <  4:285714/149‑1 (MQ=255)                                                                                                                              gtc‑gAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGc                           >  8:49239/1‑141 (MQ=255)                                                                                                                              gtc‑gAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGc                           <  7:49239/141‑1 (MQ=255)                                                                                                                                     gggCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCg             <  8:225114/149‑1 (MQ=255)                                                                                                                                       gCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGcc              <  6:143505/146‑1 (MQ=255)                                                                                                                                       gCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGcc              >  5:143505/1‑146 (MQ=255)                                                                                                                                        ttcgacaTCGCCCAGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAg                                                                   >  2:258073/5‑92 (MQ=255)                                                                                                                                        ttcgacaTCGCCCAGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAg                                                                   <  1:258073/88‑1 (MQ=255)                                                                                                                                        ttcgacaTCGCCCAGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGcac                                            <  6:72380/111‑1 (MQ=255)                                                                                                                                        ttcgacaTCGCCCAGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGcac                                            >  5:72380/5‑115 (MQ=255)                                                                                                                                           cacaTCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGcacac       <  8:177423/149‑1 (MQ=255)                                                                                                                                              aTCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTTCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCTTTTCCCAAGCCATCATCGGCGCCGGCACACAAg    <  1:226113/149‑1 (MQ=255)                                                                                                                                                   tGGGCGATGTCGAAGATATCCGTTATT‑ACGCCGCTCGCCGCTTGCGTGAGAACCT‑GCAGACAAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGCACACAAggg  <  8:124427/146‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GACCTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCGAACCAAGGTCGGTC‑GAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATT‑ACGCCGCTCGCCGCTTGCGTGAGACCCT‑GCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGCACACAAGGG  >  NC_002947/5706967‑5707257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑