Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,712,763 T→C 10.8% F71S (TTC→TCC)  ubiB → 2‑octaprenylphenol hydroxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,712,7630TC10.8% 92.7 / 3.3 37F71S (TTC→TCC) ubiB2‑octaprenylphenol hydroxylase
Reads supporting (aligned to +/- strand):  ref base T (17/16);  new base C (2/2);  total (19/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.57e-01

GATGACCTGCTGTTCGATCAGCCCTTGCTGCCCTGGTGGCTGGCCAGCCTGCGCCTGTTGATGCCGTGGCGCTGGCTGCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACATCGCCGACGAGCTGATGCTGTTGCAGGACCGTGTGCCACCGTTCGACCCGACACAAGCCGTGGCCTTGATCGAGGAACAGCTCGGTGCG  >  NC_002947/5712615‑5712905
                                                                                                                                                    |                                                                                                                                              
gATGACCTGCTGTTCGATCAGCCCTTGCTGCCCTGGTGGCTGGCCAGCCTGCGCCTGTTGATGCCGTGGCGCTGGCTGCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCCCTGCAAGACCTGGGGCCGATCtt                                                                                                                                                >  4:20824/1‑149 (MQ=255)
  tGACCTGCTGTTCGATCAGCCCTTGCTGCCCTGGTGGCTGGCCAGCCTGCGCCTGTTGATGCCGTGGCGCTGGCTGCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTtca                                                                                                                                              <  3:110701/149‑1 (MQ=255)
            ttCGATCAGCCCTTGCTGCCCTGGTGGCTGGCCAGCCTGCGCCTGTTGATGCCGTGGCGCTGGCTGCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCg                                                                                                                                     <  5:43636/148‑1 (MQ=255)
                aTCAGCCCTTGCTGCCCTGGTGGCTGGCCAGCCTGCGCCTGTTGATGCCGTGGCGCTGGCTGCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTCCATCAAGTTCGGCCAg                                                                                                                                >  7:32824/1‑149 (MQ=255)
                aTCAGCCCTTGCTGCCCTGGTGGCTGGCCAGCCTGCGCCTGTTGATGCCGTGGCGCTGGCTGCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTCCATCAAGTTCGGCCAg                                                                                                                                >  7:27940/1‑149 (MQ=255)
                     cccTTGCTGCCCTGGTGGCTGGCCAGCCTGCGCCTGTTGATGCCGTGGCGCTGGCTGCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAgctgct                                                                                                                           >  2:272787/1‑149 (MQ=255)
                                cTGGTGGCTGGCCAGCCTGCGCCTGTTGATGCCGTGGCGCTGGCTGCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACccgcc                                                                                                                >  4:202335/1‑149 (MQ=255)
                                      gctggcCAGCCTGCGCCTGTTGATGCCGTGGCGCTGGCTGCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCcgccgc                                                                                                              <  8:259272/145‑1 (MQ=255)
                                      gctggcCAGCCTGCGCCTGTTGATGCCGTGGCGCTGGCTGCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCcgccgc                                                                                                              >  7:259272/1‑145 (MQ=255)
                                          gcCAGCCTGCGCCTGTTGATGCCGTGGCGCTGGCTGCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCt                                                                                                      <  1:272787/149‑1 (MQ=255)
                                             aGCCTGCGCCTGTTGATGCCGTGGCGCTGGCTGCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGcc                                                                                                   <  3:202335/149‑1 (MQ=255)
                                                cTGCGCCTGTTGATGCCGTGGCGCTGGCTGCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGccccc                                                                                                >  5:216204/1‑149 (MQ=255)
                                                   cgcCTGTTGATGCCGTGGCGCTGGCTGCCACGCAAGCCCACCGAACTCAGCCATGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCg                                                                                              <  3:27801/148‑1 (MQ=255)
                                                    gcCTGTTGATGCCGTGGCGCTGGCTGCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCCGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGa                                                                                             >  4:292556/1‑148 (MQ=255)
                                                    gcCTGTTGATGCCGTGGCGCTGGCTGCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGa                                                                                             >  1:120591/1‑148 (MQ=255)
                                                      cTGTTGATGCCGTGGCGCTGGCTGCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACAt                                                                                          >  7:123641/1‑149 (MQ=255)
                                                        gTTGATGCCGTGGCGCTGGCTGCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCcgccgc                                                                                                              >  2:216626/1‑127 (MQ=255)
                                                        gTTGATCCCGTGGCGCTGGCTGCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCcgccgc                                                                                                              <  1:216626/127‑1 (MQ=255)
                                                                cGTGGCGCTGGCTGCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACATCGCCGACGAg                                                                                <  1:59863/149‑1 (MQ=255)
                                                                 gTGGCGCTGGCTGCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACATCGCCGACGAGc                                                                               <  8:123641/149‑1 (MQ=255)
                                                                            tgCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACATCGCCGACGAGCTGATGCTGTTg                                                                    >  2:149426/1‑149 (MQ=255)
                                                                            tgCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACATCGCCGACGAGCTGATGCTGTTg                                                                    >  8:16271/1‑149 (MQ=255)
                                                                              ccACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACATCGCCGACGAGCTGATGCTGTTg                                                                    <  3:102203/147‑1 (MQ=255)
                                                                              ccACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACATCGCCGACGAGCTGATGCTGTTGc                                                                   <  6:216204/148‑1 (MQ=255)
                                                                                        ccACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACATCGCCGACGAGCTGATGCTGTTGCAGGACCGtgtg                                                        >  5:163215/1‑149 (MQ=255)
                                                                                                cTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACATCGCCGACGAGCTGATGCTGTTGCAGGACCGTGTGCCACCGtt                                                <  1:149426/149‑1 (MQ=255)
                                                                                                     ccGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACAt                                                                                          >  5:305892/1‑102 (MQ=255)
                                                                                                     ccGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACAt                                                                                          <  6:305892/102‑1 (MQ=255)
                                                                                                      cGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACATCGCCGACGAGCTGATGc                                                                         <  2:169371/118‑1 (MQ=255)
                                                                                                      cGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACATCGCCGACGAGCTGATGc                                                                         >  1:169371/1‑118 (MQ=255)
                                                                                                          gcgcgcgcCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACATCGCCGACGAGCTGATGCTGTTGCAGGACCGTGTGCCACCGTTCGACCCGaca                                      <  2:120591/149‑1 (MQ=255)
                                                                                                                             aCTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACATCGCCGCCGAGCTGATGCTGTTGCAGGACCGTGTGCCACCGTTCGACCCGACACAAGCCGTGGCCTTGATc                    >  1:155132/1‑148 (MQ=255)
                                                                                                                                 cAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACATCGCCGACGAGCTGATGCTGTTGCAGGACCGTGTGCCACCGTTCGACCCGACACAAGCCGTGGCCTTGATCGAgg                >  5:24116/1‑148 (MQ=255)
                                                                                                                                 cAAGACCTGGGGCCGATCTCCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACATCGCCGACGAGCTGATGCTGTTGCAGGACCGTGTGCCACCGTTCGACCCGACACAAGCCGTGGCCTTGATCGAg                 <  8:27940/147‑1 (MQ=255)
                                                                                                                                 cAAGACCTGGGGCCGATCTCCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACATCGCCGACGAGCTGATGCTGTTGCAGGACCGTGTGCCACCGTTCGACCCGACACAAGCCGTGGCCTTGATCGAg                 <  8:32824/147‑1 (MQ=255)
                                                                                                                                          gggCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACATCGCCGACGAGCTGATGCTGTTGCAGGACCGTGTGCCACCGTTCGACCCGACACAAGCCGTGGCCTTGATCGAGGAACAGCTc        <  6:163215/147‑1 (MQ=255)
                                                                                                                                            gCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACATCGCCGACGAGCTGATGCTGTTGCAGGACCGTGTGCCACCGTTCGACCCGACACAAGCCGTGGCCTTGATCGAGGaa              <  5:263160/139‑1 (MQ=255)
                                                                                                                                            gCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCCACATCGCCGACGAGCTGATGCTGTTGCAGGACCGTGTGCCACCGTTCGACCCGACACAAGCCGTGGCCTTGATCGAGGaa              >  6:263160/1‑139 (MQ=255)
                                                                                                                                                aTCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACATCGCCGACGAGCTGATGCTGTTGCAGGACCGTGTGCCACCGTTCGACCCGACACAAGCCGTGGCCTTGATCGAGGAACAGCTCGGTGCg  >  1:163293/1‑147 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
GATGACCTGCTGTTCGATCAGCCCTTGCTGCCCTGGTGGCTGGCCAGCCTGCGCCTGTTGATGCCGTGGCGCTGGCTGCCACGCAAGCCCACCGAACTCAGCCGTGGCGCGCGCCTGCGCCTGGCACTGCAAGACCTGGGGCCGATCTTCATCAAGTTCGGCCAGCTGCTGTCCACCCGCCGCGATCTCCTGCCCCCCGACATCGCCGACGAGCTGATGCTGTTGCAGGACCGTGTGCCACCGTTCGACCCGACACAAGCCGTGGCCTTGATCGAGGAACAGCTCGGTGCG  >  NC_002947/5712615‑5712905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑