Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,737,602 G→C 7.8% intergenic (‑157/+45) hutU ← / ← PP_5034 urocanate hydratase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,737,6020GC 7.8% 158.3 / 4.2 51intergenic (‑157/+45)hutU/PP_5034urocanate hydratase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (26/21);  new base C (2/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

CTCTGCGGTCGTCCGCGGGCGGGTATGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACC  >  NC_002947/5737456‑5737747
                                                                                                                                                  |                                                                                                                                                 
ctctGCGGTCGTCCGCGGGCGGGTATGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATAGAGCGCCGCgcgggc                                                                                                                                                 >  7:272178/1‑149 (MQ=255)
  ctGCGGTCGTCCGCGGGCGGGTATGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCgcgggcgg                                                                                                                                               <  7:274247/149‑1 (MQ=255)
   tGCGGTCGTCCGCGGGCGGGTATGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGggcggc                                                                                                                                              >  8:238057/1‑149 (MQ=255)
    gCGGTCGTCCGCGGGCGGGTATGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGgcggcg                                                                                                                                             >  5:141417/1‑149 (MQ=255)
     cGGTCGTCCGCGGGCGGGTATGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGgcgc                                                                                                                                            <  2:245208/149‑1 (MQ=255)
      ggTCGTCCGCGGGCGGGTATGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCCCGCGCGGCGCt                                                                                                                                           <  7:107203/149‑1 (MQ=255)
               cgggcgggTATGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTcac                                                                                                                                  <  8:272178/149‑1 (MQ=255)
                  gcgggTATGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCAAAgg                                                                                                                               >  8:277875/1‑149 (MQ=255)
                   cgggTATGTCGTCGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGgc                                                                                                                              <  5:84810/149‑1 (MQ=255)
                        aTGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCgcggg                                                                                                                                                  >  6:13088/1‑124 (MQ=255)
                        aTGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCgcggg                                                                                                                                                  <  5:13088/124‑1 (MQ=255)
                                   aaTATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGc                                                                                                              >  8:232138/1‑149 (MQ=255)
                                   aaTATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGc                                                                                                              >  5:242025/1‑149 (MQ=255)
                                          aaaCACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCt                                                                                                       >  6:213883/1‑149 (MQ=255)
                                                  cTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGcc                                                                                               >  3:299815/1‑149 (MQ=255)
                                                          gtatgtaCAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAg                                                                                        <  5:104714/148‑1 (MQ=255)
                                                           tatgtaCAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGtt                                                                                      >  6:50817/1‑149 (MQ=255)
                                                           tatgtaCAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGtt                                                                                      <  5:213883/149‑1 (MQ=255)
                                                                   aGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAgg                                                                              <  7:232138/149‑1 (MQ=255)
                                                                    gCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGgc                                                                             <  7:238057/149‑1 (MQ=255)
                                                                    gCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGgc                                                                             >  8:71156/1‑149 (MQ=255)
                                                                         gACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCAc                                                                        >  7:56065/1‑149 (MQ=255)
                                                                           cAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCg                                                                      <  6:141417/149‑1 (MQ=255)
                                                                              ccAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGccc                                                                   <  7:277875/149‑1 (MQ=255)
                                                                               cAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGcccc                                                                  >  3:203322/1‑149 (MQ=255)
                                                                                aaaaTAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCTCTCGATCTCACAGGCGCCAAAAAt                                                                                                                     >  3:282771/1‑97 (MQ=255)
                                                                                aaaaTAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTca                                                                                                                                   >  1:129121/1‑83 (MQ=255)
                                                                                aaaaTAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTca                                                                                                                                   <  2:129121/83‑1 (MQ=255)
                                                                                aaaaTAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAAt                                                                                                                     <  4:282771/97‑1 (MQ=255)
                                                                                aaaaTAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCt                                                                 >  4:155880/1‑149 (MQ=255)
                                                                                     aatCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGcac                                                             >  7:6653/1‑148 (MQ=255)
                                                                                     aatCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGcac                                                             >  5:65259/1‑148 (MQ=255)
                                                                                      atCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACaa                                                           <  6:242025/149‑1 (MQ=255)
                                                                                                 ccGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACAAAAATCTACAGCCGAACCTTCAGCGCCAATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACAAGCCCCAg                                                 >  7:46432/1‑148 (MQ=255)
                                                                                                       cTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGc                                                      >  5:177131/1‑137 (MQ=255)
                                                                                                       cTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGc                                                      <  6:177131/137‑1 (MQ=255)
                                                                                                        tGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGGTCGATCAGGCAGCCCCGCCCCTGCACACCCAGCCCCAGCAATtca                                          >  7:231222/1‑148 (MQ=255)
                                                                                                            ttGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATtc                                           <  1:311515/143‑1 (MQ=255)
                                                                                                            ttGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATtc                                           >  2:311515/1‑143 (MQ=255)
                                                                                                            ttGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCAtt                                     >  7:102260/1‑149 (MQ=255)
                                                                                                               agaTTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGc                                   <  4:203322/148‑1 (MQ=255)
                                                                                                                        ttGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCg                         <  3:155880/149‑1 (MQ=255)
                                                                                                                           gggTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCaa                      <  5:50817/149‑1 (MQ=255)
                                                                                                                            ggTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAg                     <  7:71156/149‑1 (MQ=255)
                                                                                                                            ggTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAg                     >  4:177272/1‑149 (MQ=255)
                                                                                                                             gTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGAACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAg                     >  2:246224/1‑148 (MQ=255)
                                                                                                                                  gATCGAGAGACGCTCGGTCGGCTCTCGATCTCACATGCTCCAAAATTCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCg               <  6:301355/149‑1 (MQ=255)
                                                                                                                                   aTCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCg               >  3:122979/1‑148 (MQ=255)
                                                                                                                                    tCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGaa             >  1:230209/1‑149 (MQ=255)
                                                                                                                                     cGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAAtt                                            >  5:302312/1‑117 (MQ=255)
                                                                                                                                     cGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAAtt                                            <  6:302312/117‑1 (MQ=255)
                                                                                                                                     cGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGTACCGCCCCTGCACACCCCGCCCCAGCAATTCATCCTTGCCTTCCAGCCGCCAGCCGTCGaa             >  6:314802/1‑148 (MQ=38)
                                                                                                                                      gAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGaac            >  8:270925/1‑148 (MQ=255)
                                                                                                                                       aGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGcac                                                             <  5:268651/98‑1 (MQ=255)
                                                                                                                                       aGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGcac                                                             >  6:268651/1‑98 (MQ=255)
                                                                                                                                          gccgccCGCGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAAccc       <  7:270925/149‑1 (MQ=255)
                                                                                                                                               gcgggcggCGCTCGATCTCACAGGCGCCAAAAATCAACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAAcc  >  1:207789/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTCTGCGGTCGTCCGCGGGCGGGTATGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACC  >  NC_002947/5737456‑5737747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑