| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,762,503 | T→A | 6.9% | intergenic (+19/‑122) | gpmI → / → PP_5057 | 2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase/M23/M37 family peptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,762,503 | 0 | T | A | 6.9% | 166.4 / 2.6 | 58 | intergenic (+19/‑122) | gpmI/PP_5057 | 2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase/M23/M37 family peptidase |
| Reads supporting (aligned to +/- strand): ref base T (26/28); new base A (2/2); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01 | |||||||||||
GGGCAAGCGCAACGTGAAAGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTGGTACGCCAAACCCCATGCTTCGCGCCCTGATCCTCC > NC_002947/5762358‑5762646 | gggCAAGCGCAACGTGAAAGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGtgg > 8:135575/1‑146 (MQ=255) aaGCGCAACGTGAAAGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGcc < 3:257219/149‑1 (MQ=255) aaGCGCAACGTGAAAGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGcc > 3:137398/1‑149 (MQ=255) aaGCGCAACGTGAAAGGGCGCGAGGGCGGCGTTCTTGCCGTCGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCTATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGcc < 6:220389/149‑1 (MQ=255) gcgcAACGTGAAAGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGccc > 6:170573/1‑148 (MQ=255) gcgcAACGTGAAAGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCAACGCAGcccc < 2:182356/149‑1 (MQ=255) aaCGTGAAAGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAAtt < 7:171403/149‑1 (MQ=255) aGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGATCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATg > 4:44842/1‑148 (MQ=255) gTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGaa > 1:104827/1‑149 (MQ=255) tGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAAt < 1:16765/149‑1 (MQ=255) cgcgAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCAGCTGGGGCTGGAAAAGCCGGTGGAAATGCCCGGGACCTCGATTCTGGTCGCCGCCTGAATGTCGAACGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTc > 3:234652/1‑149 (MQ=255) gcgAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGATGCAGCCCCAATTGCACATGAATTc > 6:149416/1‑148 (MQ=255) cgAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCa < 4:192240/149‑1 (MQ=255) gTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGcc < 2:272010/149‑1 (MQ=255) tGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGccc < 2:169509/149‑1 (MQ=255) gCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGATAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTg > 2:113350/1‑149 (MQ=255) gCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTg > 2:145236/1‑149 (MQ=255) aCGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATc < 5:170573/149‑1 (MQ=255) gcgcCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAg > 5:39098/1‑149 (MQ=255) ccAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGa < 3:110046/149‑1 (MQ=255) ccAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGa < 2:104827/149‑1 (MQ=255) cAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGATGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAg < 5:149416/149‑1 (MQ=255) ccATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCg < 5:317209/149‑1 (MQ=255) cATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGt > 8:122795/1‑149 (MQ=255) cTGCAGCTGCTGGGGCTGTATATGCCGGTGGAAATGTCCGGTACATCGATTCTGGTCGTCGCGTGAATGTCGAACGGGGCTGCGTGGCAGCCCCATTTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGttt < 4:234652/147‑1 (MQ=255) gctgctGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCTCGCTGCGGATGCAGGGGGGCGtttgt > 3:50350/1‑142 (MQ=38) gctgctGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCTCGCTGCGGATGCAGGGGGGCGtttgt < 4:50350/144‑3 (MQ=38) ggCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGccc > 8:281931/1‑147 (MQ=255) ggCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGccc < 7:281931/147‑1 (MQ=255) gAAAAGCCGGAGGAAATGACCCGAACCTCGAGTCTGGTCGACGCCAGAATGTCGAAAGGGGCTGCGTTGCAGACCCAATTGAACATGAATTCCAGACAAAAGCACCCCTGCATCAGCAGAGAGGCGTTTTATTTGCCTGCCCacccggc > 5:168550/1‑143 (MQ=255) aaaGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCa > 5:44770/1‑149 (MQ=255) aaGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCAt < 5:247838/149‑1 (MQ=255) gTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGTGCTGCGTTGCAGCCCCAATTCCACAAGAATTCCAGACAAACGCCCCCCTGCATCAGCAGCGAGGCGTTTTTTTTGCCATCCCACCCGGGCATAcaaag > 2:237380/1‑145 (MQ=255) aaTGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGtt > 4:23793/1‑114 (MQ=255) aaTGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGtt < 3:23793/114‑1 (MQ=255) aTGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGtc < 6:39098/149‑1 (MQ=255) tGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGtct < 1:113350/149‑1 (MQ=255) aCCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTcc < 4:137398/149‑1 (MQ=255) ccTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCtt < 3:44842/148‑1 (MQ=255) cTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTgg > 1:190405/1‑149 (MQ=255) tCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTGGTACGc > 1:76570/1‑148 (MQ=255) ggTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTGGTACGCCaaa > 2:24107/1‑149 (MQ=255) gTCGACGCATGAATGTCGAAAGGGGCTCCGTAGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTGGTACGCCAAAc > 4:85498/1‑149 (MQ=255) cTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTGGTAc < 7:196436/134‑1 (MQ=255) gAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGc < 1:13842/113‑1 (MQ=255) gAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGc > 2:13842/1‑113 (MQ=255) gTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTGGTACGCCAAACCCCATGCTTCgcgc > 5:201676/1‑149 (MQ=255) gTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGACGGGCATa > 6:302876/1‑103 (MQ=255) tCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATTAATTCCAGACATACGCCCGCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCATGCGGGCATTCTAGGCCAGTCTCCATCCTTGGTCCGCCAAACCCCATGCTTCGCGcc < 6:168550/149‑1 (MQ=255) cGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTGGTACGCCAAACCCCATGCTTCGCGcc > 2:278867/1‑148 (MQ=255) gAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTGGTACGCCAAACCCCATGCTTCGCGccc < 2:76570/148‑1 (MQ=255) ggggCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTGGTACGCCAAACCCCATGCTTCGCGCCCTGAtc < 1:32027/149‑1 (MQ=255) gggCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTGGTACGCCAAACCCCATGCTTCGCGCCCTGAtcc < 1:145236/149‑1 (MQ=255) gggCTGCAACGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTGGTACGCCAAACCCCATGCTTCGCGCCCTGAtc > 4:90289/1‑148 (MQ=255) gCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTGGTACGCCAAACCCCATGCTTCGCGCCCTGAtcctc < 7:122795/149‑1 (MQ=255) cTGCGTTGCAGCCCCAATTGCACATGAATTCCTGACAAACGCCCCCCTGCATCCGCAGCGAgg > 5:176012/1‑63 (MQ=255) cTGCGTTGCAGCCCCAATTGCACATGAATTCCTGACAAACGCCCCCCTGCATCCGCAGCGAgg < 6:176012/63‑1 (MQ=255) tGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTGGTACGCCAAACCCCATGCTTCGCGCCCTGAtcctcc > 8:67477/1‑148 (MQ=255) tGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTGGTACGCCAAACCCCATGCTTCGCGCCCTGAtcctca > 4:39036/1‑147 (MQ=255) | GGGCAAGCGCAACGTGAAAGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCAATTGCACATGAATTCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTGGTACGCCAAACCCCATGCTTCGCGCCCTGATCCTCC > NC_002947/5762358‑5762646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |