Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,767,350	C→A	5.6%	M1174I (ATG→ATT) ‡	PP_5060 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,767,350	0	C	A	5.6%	227.4 / 3.6	71	M1174I (ATG→ATT) ‡	PP_5060	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (35/32);  new base A (2/2);  total (37/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01

AGGCAAGTCACAGAACATCAGCAGATGATGCGTTTGTGCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCATGTCGATGTCGGTGATGGCCAACAGGCGATCCAGCTGGCTGGAGACATTGA  >  NC_002947/5767212‑5767495                                                                                                                                            |                                                                                                                                                    aGGCAAGTCACAGAACATCAGCAGATGATGCGTTTGTGCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTgg                                                                                                                                            >  5:199193/1‑148 (MQ=255) aGGCAAGTCACAGAACATCAGCAGATGATGCGTTTGTGCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCATGGGCCTggt                                                                                                                                           >  2:260930/1‑148 (MQ=255) aGGCAAGTCACAGAACATCAGCAGATGATGCGTTTGTGCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCATGGGCCTggt                                                                                                                                           <  1:260930/149‑2 (MQ=255)   gCAAGTCACAGAACATCAGCAGATGATGCGTTTGTGCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGaa                                                                                                                                          >  3:183294/1‑148 (MQ=255)   gCAAGTCACAGAACATCAGCAGATGATGCGTTTGTGCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGaa                                                                                                                                          >  5:78702/1‑148 (MQ=255)           catacaaTCCGCAGATGATGGGCTGGGGCGGCCACCCGCATACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGAt                                                                                                                                 <  4:46513/143‑1 (MQ=255)           caGAACATCAGCAGATGATGCGTTTGTGCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGAt                                                                                                                                 <  1:123141/149‑1 (MQ=255)           caGAACATCAGCAGATGATGCGTTTGTGCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGAt                                                                                                                                 <  1:156223/149‑1 (MQ=255)                cATCAGCAGATGATGCGTTTGTGCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGa                                                                                                                            >  7:69653/1‑149 (MQ=255)                   cagcagATGATGCGTTTGTGCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGaa                                                                                                                                          >  1:137679/1‑132 (MQ=255)                   cagcagATGATGCGTTTGTGCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGaa                                                                                                                                          <  2:137679/132‑1 (MQ=255)                     gcagATGATGCGTTTGTGCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTg                                                                                                                       >  6:196509/1‑149 (MQ=255)                     gcagATGATGCGTTTGTGCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTg                                                                                                                       <  6:211280/149‑1 (MQ=255)                           gatgCGTTTGTGCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCATGGGCCTgg                                                                                                                                            >  1:10971/1‑122 (MQ=255)                           gatgCGTTTGTGCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCATGGGCCTgg                                                                                                                                            <  2:10971/122‑1 (MQ=255)                            atgCGTTTGTGCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCgg                                                                                                                                   <  8:134823/130‑1 (MQ=255)                            atgCGTTTGTGCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCgg                                                                                                                                   >  7:134823/1‑130 (MQ=255)                            atgCGTTTGTGCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGtt                                                                                                                              >  6:27435/1‑135 (MQ=255)                            atgCGTTTGTGCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGtt                                                                                                                              <  5:27435/135‑1 (MQ=255)                               cGTTTGTGCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TAc                                                                                                           <  6:241082/149‑1 (MQ=255)                                gTTTGTGCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAAGGATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TAc                                                                                                           >  1:232716/1‑149 (MQ=255)                                   tgtgCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCt                                                                                                       >  2:98714/1‑149 (MQ=255)                                      gCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTcgc                                                                                                    >  7:124710/1‑149 (MQ=255)                                          ccACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGgtcg                                                                                                                   >  5:50093/1‑132 (MQ=255)                                          ccACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGgtcg                                                                                                                   <  6:50093/132‑1 (MQ=255)                                          ccACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGa                                                                                                  <  2:222236/147‑1 (MQ=255)                                          ccACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGa                                                                                                  >  1:222236/1‑147 (MQ=255)                                          ccACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGAt                                                                                                 >  7:238783/1‑148 (MQ=255)                                               cGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCt                                                                                           <  8:124710/149‑1 (MQ=255)                                                 cAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCg                                                                                         <  6:78702/149‑1 (MQ=255)                                                    aCGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCgaga                                                                                      <  8:69653/149‑1 (MQ=255)                                                      gCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACg                                                                                    >  2:179225/1‑149 (MQ=255)                                                          aCTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTg                                                                                                                                             >  1:29647/1‑90 (MQ=255)                                                          aCTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGt                                                                                  <  5:196509/147‑1 (MQ=255)                                                            tGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCa                                                                                >  3:123481/1‑147 (MQ=255)                                                                  gcgcATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTa                                                                        <  6:199193/149‑1 (MQ=255)                                                                      aTGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGa                                                                    <  4:183294/149‑1 (MQ=255)                                                                                     tGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTg                                                       >  8:59511/1‑148 (MQ=255)                                                                                               ggTAGGCGTGCCTTTGTGCCACTGCAGGGCCCGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGAGAATGCTCGAGACGGTCAGAGTGGTAGTGACGGAAGGCAGTTGCctgtcgatgt                                            >  4:200095/1‑149 (MQ=255)                                                                                               ggTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACtt                                                        >  7:8874/1‑137 (MQ=255)                                                                                               ggTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACtt                                                        <  8:8874/137‑1 (MQ=255)                                                                                                   ggCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCATGTCGATGTCGGt                                        >  3:120315/1‑149 (MQ=255)                                                                                                    gCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCATGTCGATGTCGGt                                        >  5:62209/1‑148 (MQ=255)                                                                                                      gTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCat                                                    >  8:100390/1‑134 (MQ=255)                                                                                                      gTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCat                                                    <  7:100390/134‑1 (MQ=255)                                                                                                      gTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCATGTCGATGTCGGTGAt                                     >  3:302244/1‑149 (MQ=255)                                                                                                        gCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCt                                                                                           <  1:311668/93‑1 (MQ=255)                                                                                                        gCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCt                                                                                           >  2:311668/1‑93 (MQ=255)                                                                                                        gCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAgg                                                             >  4:158852/1‑123 (MQ=255)                                                                                                        gCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAgg                                                             <  3:158852/123‑1 (MQ=255)                                                                                                         ccTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCATGTCGATGTCGGTTATGGc                                  <  6:62209/149‑1 (MQ=255)                                                                                                          cTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCatgtcgat                                              >  2:73881/1‑136 (MQ=255)                                                                                                          cTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCatgtcgat                                              <  1:73881/136‑1 (MQ=255)                                                                                                          cTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCATGTCGATGTCGGTGATggg                                  >  8:229589/1‑147 (MQ=255)                                                                                                                 ccACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCATGTCGATGTCGGTGATGGCCAACAGGc                          <  4:120315/149‑1 (MQ=255)                                                                                                                   aCTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACtt                                                        >  2:118976/1‑117 (MQ=255)                                                                                                                   aCTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACtt                                                        <  1:118976/117‑1 (MQ=255)                                                                                                                   aCTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCATGTCGATGTCGGTGATGGCCAACAGGCGa                        <  2:232716/149‑1 (MQ=255)                                                                                                                     tGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCATGTCGATGTCGGTGATGGCCAACAGGcaga                       >  5:163618/1‑145 (MQ=255)                                                                                                                          ggCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCTTCTTGGGCTCGCGATATTGTTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCATGTCGATGTCGGTGATGGCCAACAGGCGATCCAg                   <  1:98714/149‑1 (MQ=255)                                                                                                                          ggCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCatgtcga                                               <  7:111444/119‑1 (MQ=255)                                                                                                                          ggCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCatgtcga                                               >  8:111444/1‑119 (MQ=255)                                                                                                                            ccAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCATGTCGATGTCGGTGAt                                     <  1:298514/127‑1 (MQ=255)                                                                                                                            ccAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCATGTCGATGTCGGTGAt                                     >  2:298514/1‑127 (MQ=255)                                                                                                                               gCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCATGTCGATGTCGGTGATGGCCAACAGGCGATCCAgctggctg            <  8:238783/149‑1 (MQ=255)                                                                                                                                cTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCATGTCGATGTCGGTGATGGCCAACAGGCGATCCAgctggctg            <  7:13368/148‑1 (MQ=255)                                                                                                                                cTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCATGTCGATGTCGGTGATGGCCAACAGGCGATCCAgctggctg            >  8:13368/1‑148 (MQ=255)                                                                                                                                   gACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACg                                                                                    <  5:297024/73‑1 (MQ=255)                                                                                                                                   gACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACg                                                                                    >  6:297024/1‑73 (MQ=255)                                                                                                                                       aGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCATGTCGATGTCGGTGATg                                    <  4:519/117‑1 (MQ=255)                                                                                                                                         gCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCATGTCGATGTCGGTGATGGCCAACAGGCGATCCAGCTGGCTGGAGACATtga  >  7:288603/1‑149 (MQ=255)                                                                                                                                          cccATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCATGTCGATGTCgg                                         <  8:165890/109‑1 (MQ=255)                                                                                                                                          cccATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCATGTCGATGTCgg                                         >  7:165890/1‑109 (MQ=255)                                                                                                                                            |                                                                                                                                                    AGGCAAGTCACAGAACATCAGCAGATGATGCGTTTGTGCGGCCACCCGCAGACGCTGACTGGCGAGCGCATGGCGGAA‑GATCATGTAGTGCTGGGTAGGCGTGCCTTTGTGCCACTGCAGGGCCAGCTCGACCAGGCCCATGGCCTGGAAAAACCGGATGTTGATCGTGGTCGTCT‑‑TACGGCTCGCGATAATGCTCGAGACGGTCAGAGTGGTATTGACGTAAGGCACTTGCATGTCGATGTCGGTGATGGCCAACAGGCGATCCAGCTGGCTGGAGACATTGA  >  NC_002947/5767212‑5767495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑