Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,801,457 G→T 11.4% P45Q (CCG→CAG) ‡ aroK ← shikimate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,801,4570GT11.4% 115.0 / 7.5 44P45Q (CCG→CAG) ‡aroKshikimate kinase
Reads supporting (aligned to +/- strand):  ref base G (22/17);  new base T (2/3);  total (24/20)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.19e-01

ACCCTGATGCAGCGCCTGCCGGTTGGCTTCGCGCATCACCGCGCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTCGCACAGAATCAACGACT  >  NC_002947/5801309‑5801603
                                                                                                                                                    |                                                                                                                                                  
aCCCTGATGCAGCGCCTGCCGGTTGGCTTCGCGCATCACCGCGCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACg                                                                                                                                                    <  7:281720/149‑1 (MQ=255)
 cccTGATGCAGCGCCTGCCGGTTGGCTTCGCGCATCACCGCGCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACgg                                                                                                                                                   <  3:169385/149‑1 (MQ=255)
  ccTGATGCAGCGCCTGCCGGTTGGCTTCGCGCATCACCGCGCCACCGCCGGTGGCCAGCCCCACACCGTCGAGCGCGCACAACTCGGCAATCAGAGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACggg                                                                                                                                                  >  8:79061/1‑149 (MQ=255)
  ccTGATGCAGCGCCTGCCGGTTGGCTTCGCGCATCACCGCGCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACgg                                                                                                                                                   >  4:37189/1‑148 (MQ=255)
          aGCGCCTGCCGGTTGGCTTCGCGCATCACCGCGCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAACATCCCGTGGATATTcga                                                                                                                                          <  6:87995/149‑2 (MQ=255)
           gcgcCTGCCGGTTGGCTTCGCGCATCACCGCCCCACCGCCGGTGGCCAGCACCACACCCTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCATACTTGTCGAATATCCACGGGATGATcgcg                                                                                                                                         >  4:99284/1‑149 (MQ=255)
               cTGCCGGTTGGCTTCGCGCATCACCGCGCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACgg                                                                                                                                                   >  1:304347/1‑135 (MQ=255)
               cTGCCGGTTGGCTTCGCGCATCACCGCGCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACgg                                                                                                                                                   <  2:304347/135‑1 (MQ=255)
                      ttGGCTTCGCGCATCACCGCGCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCAAGGGATGTTAGCGCCGCATCGCAg                                                                                                                              >  7:105976/1‑149 (MQ=255)
                          cTTCGCGCATCACCGCGCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTc                                                                                                                           >  1:283849/1‑148 (MQ=255)
                          cTTCGCGCATCACCGCGCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAACATCCCGTGGATATTcgac                                                                                                                                         <  8:99066/134‑3 (MQ=255)
                          cTTCGCGCATCACCGCGCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAACATCCCGTGGATATTcgac                                                                                                                                         >  7:99066/1‑132 (MQ=255)
                                     aCCGCGCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGt                                                                                                                <  3:287167/148‑1 (MQ=255)
                                       cgcgCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCg                                                                                                              >  5:54859/1‑148 (MQ=255)
                                       cgcgCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAACATCCCGTGGATATTcga                                                                                                                                          >  4:189556/1‑119 (MQ=255)
                                       cgcgCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAACATCCCGTGGATATTcga                                                                                                                                          <  3:189556/120‑2 (MQ=255)
                                            cACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATcc                                                                                                        >  6:53647/1‑149 (MQ=255)
                                            cACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATcc                                                                                                        >  5:85997/1‑149 (MQ=255)
                                            cACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATcc                                                                                                        <  3:37189/149‑1 (MQ=255)
                                             aCCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCt                                                                                                       >  7:202474/1‑149 (MQ=255)
                                              ccgccgGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCtt                                                                                                      <  6:85997/149‑1 (MQ=255)
                                              ccgccgGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCtt                                                                                                      <  4:277127/149‑1 (MQ=255)
                                                  cgGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATcc                                                                                                        <  5:37475/143‑1 (MQ=255)
                                                  cgGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGAATCGCAGTTCGATTTCCTTGTCGGAATcc                                                                                                        >  6:37475/1‑143 (MQ=255)
                                                     tGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGAtt                                                                                                                       <  6:258619/125‑1 (MQ=255)
                                                     tGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGCCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGAtt                                                                                                                       >  5:258619/1‑125 (MQ=255)
                                                        ccAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAgg                                                                                            <  6:54859/149‑1 (MQ=255)
                                                        ccAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAgg                                                                                            <  8:175460/149‑1 (MQ=255)
                                                         cAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGgc                                                                                           >  4:198997/1‑149 (MQ=255)
                                                                 caacGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCGCGCCTTCCTTGTCGAATATCCTCGGGATGTTCGCTCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGctct                                                                                   <  1:86133/146‑1 (MQ=255)
                                                                  acCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCtt                                                                                  >  5:181071/1‑149 (MQ=255)
                                                                   ccGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCttt                                                                                 <  6:181071/149‑1 (MQ=255)
                                                                              gcACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGc                                                                      <  2:283849/149‑1 (MQ=255)
                                                                                       ggCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTAGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACATCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATgg                                                             >  2:218185/1‑149 (MQ=255)
                                                                                                       tCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGcc                                             >  3:156614/1‑149 (MQ=255)
                                                                                                           gATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATg                                         >  7:23975/1‑149 (MQ=255)
                                                                                                                cGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGccc                                    <  7:79061/149‑1 (MQ=255)
                                                                                                                       ccGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAg                              >  2:179097/1‑148 (MQ=255)
                                                                                                                              gcattccttGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCaaa                       <  1:218185/145‑1 (MQ=255)
                                                                                                                              gCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCaaa                       <  4:156614/148‑1 (MQ=255)
                                                                                                                                cttccttGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAAttt                    >  2:105513/1‑149 (MQ=255)
                                                                                                                                    cttGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTCGca                <  5:53647/149‑1 (MQ=255)
                                                                                                                                    cttGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTCGca                <  1:179097/149‑1 (MQ=255)
                                                                                                                                       gTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTCGca                >  7:58717/1‑146 (MQ=255)
                                                                                                                                          gAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTc                   >  2:275981/1‑140 (MQ=255)
                                                                                                                                          gAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTc                   <  1:275981/140‑1 (MQ=255)
                                                                                                                                          gAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTCGCACAGAAt          >  1:238828/1‑149 (MQ=255)
                                                                                                                                                ccACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTCGCACAGAATCAACGa    >  4:213882/1‑149 (MQ=255)
                                                                                                                                                ccACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTCGCACAGAATCAACGa    >  1:148592/1‑149 (MQ=255)
                                                                                                                                                   cGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTCGCACAGAATCAACGACt  >  5:192234/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ACCCTGATGCAGCGCCTGCCGGTTGGCTTCGCGCATCACCGCGCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTCGCACAGAATCAACGACT  >  NC_002947/5801309‑5801603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑