Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,801,462 G→A 10.6% N43N (AAC→AAT)  aroK ← shikimate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,801,4620GA10.6% 123.9 / 5.2 47N43N (AAC→AAT) aroKshikimate kinase
Reads supporting (aligned to +/- strand):  ref base G (23/19);  new base A (2/3);  total (25/22)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

AGCGCCTGCCGGTTGGCTTCGCGCATCACCGCGCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTCGCACAGAATCAACGACTCACA  >  NC_002947/5801319‑5801607
                                                                                                                                               |                                                                                                                                                 
aGCGCCTGCCGGTTGGCTTCGCGCATCACCGCGCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAACATCCCGTGGATATTcga                                                                                                                                              <  6:87995/149‑2 (MQ=255)
 gcgcCTGCCGGTTGGCTTCGCGCATCACCGCCCCACCGCCGGTGGCCAGCACCACACCCTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCATACTTGTCGAATATCCACGGGATGATcgcg                                                                                                                                             >  4:99284/1‑149 (MQ=255)
            ttGGCTTCGCGCATCACCGCGCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCAAGGGATGTTAGCGCCGCATCGCAg                                                                                                                                  >  7:105976/1‑149 (MQ=255)
                cTTCGCGCATCACCGCGCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTc                                                                                                                               >  1:283849/1‑148 (MQ=255)
                cTTCGCGCATCACCGCGCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAACATCCCGTGGATATTcgac                                                                                                                                             <  8:99066/134‑3 (MQ=255)
                cTTCGCGCATCACCGCGCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAACATCCCGTGGATATTcgac                                                                                                                                             >  7:99066/1‑132 (MQ=255)
                           aCCGCGCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGt                                                                                                                    <  3:287167/148‑1 (MQ=255)
                             cgcgCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCg                                                                                                                  >  5:54859/1‑148 (MQ=255)
                             cgcgCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAACATCCCGTGGATATTcga                                                                                                                                              >  4:189556/1‑119 (MQ=255)
                             cgcgCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAACATCCCGTGGATATTcga                                                                                                                                              <  3:189556/120‑2 (MQ=255)
                                  cACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATcc                                                                                                            >  6:53647/1‑149 (MQ=255)
                                  cACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATcc                                                                                                            >  5:85997/1‑149 (MQ=255)
                                  cACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATcc                                                                                                            <  3:37189/149‑1 (MQ=255)
                                   aCCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCt                                                                                                           >  7:202474/1‑149 (MQ=255)
                                    ccgccgGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCtt                                                                                                          <  4:277127/149‑1 (MQ=255)
                                    ccgccgGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCtt                                                                                                          <  6:85997/149‑1 (MQ=255)
                                        cgGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATcc                                                                                                            <  5:37475/143‑1 (MQ=255)
                                        cgGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGAATCGCAGTTCGATTTCCTTGTCGGAATcc                                                                                                            >  6:37475/1‑143 (MQ=255)
                                           tGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGAtt                                                                                                                           <  6:258619/125‑1 (MQ=255)
                                           tGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGCCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGAtt                                                                                                                           >  5:258619/1‑125 (MQ=255)
                                              ccAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAgg                                                                                                <  8:175460/149‑1 (MQ=255)
                                              ccAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAgg                                                                                                <  6:54859/149‑1 (MQ=255)
                                               cAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGgc                                                                                               >  4:198997/1‑149 (MQ=255)
                                                       caacGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCGCGCCTTCCTTGTCGAATATCCTCGGGATGTTCGCTCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGctct                                                                                       <  1:86133/146‑1 (MQ=255)
                                                        acCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCtt                                                                                      >  5:181071/1‑149 (MQ=255)
                                                         ccGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCttt                                                                                     <  6:181071/149‑1 (MQ=255)
                                                                    gcACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGc                                                                          <  2:283849/149‑1 (MQ=255)
                                                                             ggCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTAGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACATCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATgg                                                                 >  2:218185/1‑149 (MQ=255)
                                                                                             tCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGcc                                                 >  3:156614/1‑149 (MQ=255)
                                                                                                 gATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATg                                             >  7:23975/1‑149 (MQ=255)
                                                                                                      cGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGccc                                        <  7:79061/149‑1 (MQ=255)
                                                                                                             ccGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAg                                  >  2:179097/1‑148 (MQ=255)
                                                                                                                    gcattccttGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCaaa                           <  1:218185/145‑1 (MQ=255)
                                                                                                                    gCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCaaa                           <  4:156614/148‑1 (MQ=255)
                                                                                                                      cttccttGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAAttt                        >  2:105513/1‑149 (MQ=255)
                                                                                                                          cttGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTCGca                    <  5:53647/149‑1 (MQ=255)
                                                                                                                          cttGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTCGca                    <  1:179097/149‑1 (MQ=255)
                                                                                                                             gTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTCGca                    >  7:58717/1‑146 (MQ=255)
                                                                                                                                gAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTc                       >  2:275981/1‑140 (MQ=255)
                                                                                                                                gAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTc                       <  1:275981/140‑1 (MQ=255)
                                                                                                                                gAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTCGCACAGAAt              >  1:238828/1‑149 (MQ=255)
                                                                                                                                      ccACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTCGCACAGAATCAACGa        >  1:148592/1‑149 (MQ=255)
                                                                                                                                      ccACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTCGCACAGAATCAACGa        >  4:213882/1‑149 (MQ=255)
                                                                                                                                         cGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTCGCACAGAATCAACGACt      >  5:192234/1‑148 (MQ=255)
                                                                                                                                           ggATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTCGCACAGAATCAACGACTcac   <  3:213882/149‑1 (MQ=255)
                                                                                                                                            gATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTCGCACAGAATCAACGACTcaca  <  3:99284/149‑1 (MQ=255)
                                                                                                                                            gATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTCGCACAGAATCAACGACTca    >  2:141505/1‑147 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
AGCGCCTGCCGGTTGGCTTCGCGCATCACCGCGCCACCGCCGGTGGCCAGCACCACACCGTCGAGCGCGCACAACTCGGCAATCATCGCTTGCTCGCGATCACGGAAACCCGGCTCGCCTTCCTTGTCGAATATCCACGGGATGTTCGCGCCGCATCGCAGTTCGATTTCCTTGTCGGAATCCTTGAACAGCAGGCGCAGCTCTTTGGCCAACAGGCGTCCGATGGTGCTTTTACCAGCGCCCATGGGCCCCACAAGTATCAAATTTCGCACAGAATCAACGACTCACA  >  NC_002947/5801319‑5801607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑