Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	536,280	A→G	8.0%	noncoding (178/190 nt)	PP_mr06 ←	P27 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	536,280	0	A	G	8.0%	176.0 / 4.9	62	noncoding (178/190 nt)	PP_mr06	P27 ncRNA
Reads supporting (aligned to +/- strand):  ref base A (29/28);  new base G (2/3);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

AAGCCCTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGG  >  NC_002947/536134‑536419                                                                                                                                                   |                                                                                                                                            aaGCCCTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGt                                                                                                                                           >  5:178072/1‑149 (MQ=255)  aGCCCTGATAGCTGAACTGAAGCGTATCAAGCCACCTTCCTCGAAAACTATCTACGATAAGCGCGTTACCCTTACCACCACCATGGGCCCAGCTCTCGTCATCTATCATAGCTCGCTGAACGTGTAAGAACATGGAGGCGCGACAAGTc                                                                                                                                          >  8:58078/1‑149 (MQ=255)   gCCCTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTcg                                                                                                                                         <  4:241238/149‑1 (MQ=255)       tGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGcgcc                                                                                                                                     >  7:211756/1‑149 (MQ=255)         aTCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTCTCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGGCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACTGGTCGcgccgc                                                                                                                                   <  5:207313/149‑1 (MQ=255)         aTCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACTGGTCGcgcc                                                                                                                                     <  4:58031/147‑1 (MQ=255)          tcgttttccTGAAGCGTATGAAGCCAGCTTCCTCGAAAGGTATCTACTTTAAGCGCGTTACCCTTAGCACCACCATGGGCAGAGGTTTGGTCATTGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGAGTGGTCGCgcctcc                                                                                                                                  <  4:67478/142‑1 (MQ=255)                   tGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAAt                                                                                                                         >  6:120488/1‑149 (MQ=255)                     aaGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAAt                                                                                                                         <  8:48585/147‑1 (MQ=255)                     aaGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTg                                                                                                                       >  6:91627/1‑149 (MQ=255)                               aGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCcctgc                                                                                                             >  2:308691/1‑149 (MQ=255)                                 ccAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCcctgcct                                                                                                           <  4:114808/149‑1 (MQ=255)                                      ttCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTg                                                                                                                       <  5:207510/132‑1 (MQ=255)                                      ttCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTg                                                                                                                       >  6:207510/1‑132 (MQ=255)                                      ttCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGAACAGAGCTCGATGAACGTGTAAGAACATGGCGGCGAGACCAGTCGCGACGCCAGCAAAAATTGGGGTCCCTGCCTGGCgg                                                                                                      >  3:35477/1‑149 (MQ=255)                                        ccTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCggg                                                                                                     <  7:257036/148‑1 (MQ=255)                                               aGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATcc                                                                                             <  4:275920/149‑1 (MQ=255)                                               aGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATc                                                                                              <  1:140824/148‑1 (MQ=255)                                               aGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATc                                                                                              >  2:140824/1‑148 (MQ=255)                                                 gTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATcc                                                                                             >  6:192412/1‑147 (MQ=255)                                                      tACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCg                                                                                      >  6:6404/1‑149 (MQ=255)                                                        cGTTAAGCGCGTTACCCTGAGCACCACCCTGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTa                                                                                    <  5:190956/149‑1 (MQ=255)                                                          ttAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAgg                                                                                  <  3:147987/149‑1 (MQ=255)                                                               cgcgTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGgcggcg                                                                             >  5:231694/1‑149 (MQ=255)                                                                  gTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCggg                                                                                                     <  2:16304/122‑1 (MQ=255)                                                                      cccTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCt                                                                      >  4:47382/1‑149 (MQ=255)                                                                               ccaccaTGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACAt                                                              >  8:223632/1‑148 (MQ=255)                                                                                cacgaTGGGCCCAGGTGTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCTACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTTGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCa                                                            <  4:35477/149‑1 (MQ=255)                                                                                caccaTGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCa                                                            <  5:6404/149‑1 (MQ=255)                                                                                caccaTGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCa                                                            <  6:178072/149‑1 (MQ=255)                                                                                caccaTGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCa                                                            <  5:192412/149‑1 (MQ=255)                                                                                     tGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCa                                                       <  6:231694/149‑1 (MQ=255)                                                                                        gCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACg                                                     >  4:158724/1‑148 (MQ=255)                                                                                          ccAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAg                                                  <  1:308691/149‑1 (MQ=255)                                                                                            aGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGAt                                                >  8:103898/1‑149 (MQ=255)                                                                                             ggTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATg                                               <  5:91627/149‑1 (MQ=255)                                                                                                   gTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTc                                         <  7:277333/149‑1 (MQ=255)                                                                                                    tCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTcc                                        <  7:223632/149‑1 (MQ=255)                                                                                                     cATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTccc                                       >  8:53890/1‑149 (MQ=255)                                                                                                      aTCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCATCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTccc                                       <  7:58078/148‑1 (MQ=255)                                                                                                      aTCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGc                                                                        >  7:5551/1‑115 (MQ=255)                                                                                                      aTCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGc                                                                        <  8:5551/115‑1 (MQ=255)                                                                                                      aTCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTccc                                       >  7:130086/1‑148 (MQ=255)                                                                                                          aTCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTa                                                                                    <  7:174844/99‑1 (MQ=255)                                                                                                          aTCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTa                                                                                    >  8:174844/1‑99 (MQ=255)                                                                                                             agagCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGtt                               <  7:53890/149‑1 (MQ=255)                                                                                                             agagCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGt                                >  5:254273/1‑148 (MQ=255)                                                                                                              gagCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATg                                               <  1:268172/132‑1 (MQ=255)                                                                                                              gagCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATg                                               >  2:268172/1‑132 (MQ=255)                                                                                                               agCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTAc                             <  8:211756/149‑1 (MQ=255)                                                                                                                    gCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGTGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAAt                        >  1:315908/1‑149 (MQ=255)                                                                                                                    gCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTc                                  <  7:164727/139‑1 (MQ=255)                                                                                                                    gCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTc                                  >  8:164727/1‑139 (MQ=255)                                                                                                                         aCGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAACACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGa                    >  1:100818/1‑148 (MQ=255)                                                                                                                             gtAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGa                    <  8:139567/144‑1 (MQ=255)                                                                                                                             gtAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGa                    >  7:139567/1‑144 (MQ=255)                                                                                                                              tAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCa               >  2:28777/1‑148 (MQ=255)                                                                                                                                 gAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAAc           >  7:130969/1‑149 (MQ=255)                                                                                                                                  aaCATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAAc           >  5:180286/1‑148 (MQ=255)                                                                                                                                   aCATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGTGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGa         <  2:315908/149‑1 (MQ=255)                                                                                                                                       ggcggcGCGACTGGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATc     >  6:3207/1‑149 (MQ=255)                                                                                                                                          ggcgCGACTGGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCgg  >  3:250552/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                            AAGCCCTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGG  >  NC_002947/536134‑536419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑