Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,824,712 T→G 10.3% intergenic (+136/+38) PP_5102 → / ← trmB hypothetical protein/tRNA (guanosine(46)‑N7)‑methyltransferase TrmB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,824,7120TG10.3% 149.5 / 7.0 56intergenic (+136/+38)PP_5102/trmBhypothetical protein/tRNA (guanosine(46)‑N7)‑methyltransferase TrmB
Reads supporting (aligned to +/- strand):  ref base T (23/27);  new base G (4/2);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 4.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCCGCGGCTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGT  >  NC_002947/5824564‑5824859
                                                                                                                                                    |                                                                                                                                                   
tCGCCGCGGCTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCtt                                                                                                                                                     >  6:16332/1‑149 (MQ=255)
    cacgGCTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCa                                                                                                                                                 <  8:91406/147‑1 (MQ=255)
          tGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACGCTCCCTTCACCGTGGGGGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCgg                                                                                                                                           <  7:203484/149‑1 (MQ=255)
          tGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCgg                                                                                                                                           >  5:91390/1‑149 (MQ=255)
            aGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGtt                                                                                                                                         <  4:23552/149‑1 (MQ=255)
            aGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGt                                                                                                                                          <  2:210931/148‑1 (MQ=255)
              cGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGttt                                                                                                                                        >  3:62344/1‑148 (MQ=255)
               gAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTa                                                                                                                                      <  5:16332/149‑1 (MQ=255)
                   gACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGCGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCg                                                                                                                                   >  4:48402/1‑148 (MQ=255)
                     ccccTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGaa                                                                                                                                 >  3:145700/1‑148 (MQ=255)
                        cTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCTGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTGAAAAGGGCGGtttttt                                                                                                                                     <  7:28176/141‑3 (MQ=18)
                        cTTGTAGGAGCGGATTAAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTGAAAAGGGCGGtttttt                                                                                                                                     >  8:28176/1‑139 (MQ=18)
                           gTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGc                                                                                                                            <  2:71460/147‑1 (MQ=255)
                                   ggATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGcc                                                                                                                  >  5:268739/1‑149 (MQ=255)
                                    gATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCg                                                                                                                 >  3:256326/1‑149 (MQ=255)
                                      ttCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCtttt                                                                                                                                                   <  6:168678/113‑1 (MQ=255)
                                      ttCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCtttt                                                                                                                                                   >  5:168678/1‑113 (MQ=255)
                                       tCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGcc                                                                                                                           <  7:192927/136‑1 (MQ=255)
                                       tCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGcc                                                                                                                           >  8:192927/1‑136 (MQ=255)
                                         aaCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCaa                                                                                                            >  7:220256/1‑149 (MQ=255)
                                         aaCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCaa                                                                                                            >  1:185626/1‑149 (MQ=255)
                                         aaCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCa                                                                                                             >  8:66681/1‑148 (MQ=255)
                                            cgcgAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATc                                                                                                          >  5:177735/1‑148 (MQ=255)
                                             gcgAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGGTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCa                                                                                                        >  4:142848/1‑149 (MQ=255)
                                              cgAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCAc                                                                                                       <  6:177735/149‑1 (MQ=255)
                                              cgAAAGGGCCGGCCCTGCGGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCAc                                                                                                       <  5:224790/149‑1 (MQ=255)
                                                       cggccCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGaa                                                                                              <  8:220256/149‑1 (MQ=255)
                                                       cggccCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGaa                                                                                              <  2:185626/149‑1 (MQ=255)
                                                           ccTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTc                                                                                          <  2:272226/149‑1 (MQ=255)
                                                               ctgGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGt                                                                                      <  4:145700/149‑1 (MQ=255)
                                                               ctgGCCAATCTCCACCCGCCCTTCACCGCGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGt                                                                                      <  3:48402/149‑1 (MQ=255)
                                                                tgGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATc                                                                                                          <  4:206434/128‑1 (MQ=255)
                                                                tgGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTAAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATc                                                                                                          >  3:206434/1‑128 (MQ=255)
                                                                  gCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTcc                                                                                    >  4:23322/1‑148 (MQ=255)
                                                                  gCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTcc                                                                                    <  6:323379/148‑1 (MQ=255)
                                                                           cACCCGCCCTTCTCCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTGTTCAGGGCGGGTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTGCTCGAACTTCAAGTCCCAGACCCCgg                                                                          >  8:275798/1‑148 (MQ=38)
                                                                             cccGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCATCTCGAACTTCAAGTCCCACACCCCGTGc                                                                        <  6:268739/149‑1 (MQ=255)
                                                                              ccGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGcc                                                                       >  2:82115/1‑149 (MQ=255)
                                                                                  ccTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCCCCCGTTTAAGGGCGGTTTTACCGAAAAGGCCGTATACGCCCCGCCATGCCCCGTGTCGAACTTCAAGTACCACCCCCCCGGcccccg                                                                   >  2:300714/1‑147 (MQ=21)
                                                                                             ggCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACcg                                                         <  4:62344/148‑1 (MQ=255)
                                                                                               cgccgcTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCgcgg                                                      <  3:23322/149‑1 (MQ=255)
                                                                                                    cTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTc                                                 <  7:66681/149‑1 (MQ=255)
                                                                                                       ccGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGaa                                              <  3:227982/149‑1 (MQ=255)
                                                                                                               gcgGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGa                                      >  8:218237/1‑149 (MQ=255)
                                                                                                                cgGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCTAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGAt                                     <  1:82115/149‑1 (MQ=255)
                                                                                                                      aaCCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGt                                                   >  3:168776/1‑129 (MQ=255)
                                                                                                                      aaCCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGt                                                   <  4:168776/129‑1 (MQ=255)
                                                                                                                        cccGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGCACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGct                             >  3:231868/1‑149 (MQ=255)
                                                                                                                               cTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCggg                      >  6:98327/1‑149 (MQ=255)
                                                                                                                                   aaaGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGcc                                                                       >  6:237940/1‑96 (MQ=255)
                                                                                                                                   aaaGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGcc                                                                       <  5:237940/96‑1 (MQ=255)
                                                                                                                                     aGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGgcg                               >  6:240960/1‑134 (MQ=255)
                                                                                                                                     aGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGgcg                               <  5:240960/134‑1 (MQ=255)
                                                                                                                                       gtaaaaCCGCCCTGAAAAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGca              >  3:70250/3‑149 (MQ=255)
                                                                                                                                       aaaaaaCCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGAt                                     <  4:73272/126‑1 (MQ=255)
                                                                                                                                       aaaaaaCCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGAt                                     >  3:73272/1‑126 (MQ=255)
                                                                                                                                        taaaaCCGCCCTGAAAAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCggg                      <  7:138720/139‑1 (MQ=255)
                                                                                                                                        taaaaCCGCCCTGAAAAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCggg                      >  8:138720/2‑140 (MQ=255)
                                                                                                                                                cccTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCACAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGc     <  6:65339/149‑1 (MQ=255)
                                                                                                                                                   ttttCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGt  >  4:15726/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCGCCGCGGCTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGT  >  NC_002947/5824564‑5824859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑