Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 543,727 A→G 5.5% N656S (AAC→AGC)  rpoC → DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947543,7270AG 5.5% 218.2 / 2.1 72N656S (AAC→AGC) rpoCDNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base A (34/34);  new base G (2/2);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

CAGTGCTACCGCGTGGTTGGTCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGAAAGAGAAG  >  NC_002947/543579‑543875
                                                                                                                                                    |                                                                                                                                                    
cAGTGCTACCGCGTGGTTGGTCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTaa                                                                                                                                                      <  1:22315/149‑1 (MQ=255)
         cgcgTGGTTGGTCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGa                                                                                                                                             <  4:83097/149‑1 (MQ=255)
             tggttggtCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAATGCCCGCATCATCGGTAACGCTACCGAtgaa                                                                                                                                         <  1:25117/149‑1 (MQ=255)
                ttggtCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGAtgaagt                                                                                                                                       >  6:77681/1‑148 (MQ=255)
                   gtCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGAtgaagtgaag                                                                                                                                   <  4:200627/149‑1 (MQ=255)
                    tCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGAtgaagtgaag                                                                                                                                   <  1:293720/148‑1 (MQ=255)
                        aaaGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAAt                                                                                                                              >  1:24403/1‑149 (MQ=255)
                          agagACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTTGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATc                                                                                                                             >  1:75925/1‑148 (MQ=255)
                             gaCCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCgaga                                                                                                                         <  3:186460/149‑1 (MQ=255)
                                cGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGcc                                                                                                                      <  5:77681/149‑1 (MQ=255)
                                 gTTATCTTCGCCCACCACCTGATCTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCa                                                                                                                     <  8:105099/149‑1 (MQ=255)
                                             gACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCgg                                                                                                         <  7:160830/149‑1 (MQ=255)
                                                cAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCgaga                                                                                                                         <  4:148404/130‑1 (MQ=255)
                                                cAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCgaga                                                                                                                         >  3:148404/1‑130 (MQ=255)
                                                cAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCt                                                                                                      <  8:140199/149‑1 (MQ=255)
                                                 aaCTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCgag                                                                                                                          >  8:297499/1‑128 (MQ=255)
                                                 aaCTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCgag                                                                                                                          <  7:297499/128‑1 (MQ=255)
                                                 aaCTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTg                                                                                                     >  2:256709/1‑149 (MQ=255)
                                                          acacCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCg                                                                                                                            >  1:205993/1‑117 (MQ=255)
                                                          acacCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCg                                                                                                                            <  2:205993/117‑1 (MQ=255)
                                                          acacCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAg                                                                                            >  4:259659/1‑149 (MQ=255)
                                                            acaggTTTCGAATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAggg                                                                                          >  1:160435/4‑149 (MQ=255)
                                                            acCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAggg                                                                                          <  3:259659/149‑1 (MQ=255)
                                                                    cGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAg                                                                                   <  4:27921/148‑1 (MQ=255)
                                                                     gCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAgg                                                                                           >  8:81370/1‑139 (MQ=255)
                                                                     gCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAgg                                                                                           <  7:81370/139‑1 (MQ=255)
                                                                      cATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTa                                                                                 >  6:61284/1‑148 (MQ=255)
                                                                           tCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTAcaacaa                                                                           >  2:257981/1‑149 (MQ=255)
                                                                           tCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCAATGGCGAGAAGTacacc                                                                             >  8:244463/1‑145 (MQ=255)
                                                                            cGACCAATTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAg                                                                          <  8:207215/149‑1 (MQ=255)
                                                                             gACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAgg                                                                         >  2:141831/1‑149 (MQ=255)
                                                                              aCCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGAtg                                                                                                                                           >  5:26553/1‑82 (MQ=255)
                                                                              aCCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGAtg                                                                                                                                           <  6:26553/82‑1 (MQ=255)
                                                                                cATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCa                                                                      >  6:129193/1‑149 (MQ=255)
                                                                                  tttCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCTGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATc                                                                    <  5:129193/149‑1 (MQ=255)
                                                                                  tttCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTa                                                                                                                  <  5:179254/103‑1 (MQ=255)
                                                                                  tttCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTa                                                                                                                  >  6:179254/1‑103 (MQ=255)
                                                                                       ggCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACtt                                                               >  6:150330/1‑149 (MQ=255)
                                                                                          gTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCTGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTtgtg                                                            <  7:244463/149‑1 (MQ=255)
                                                                                                aTCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCg                                                        >  3:274667/1‑147 (MQ=255)
                                                                                                   ggTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGc                                                   >  8:214766/1‑149 (MQ=255)
                                                                                                         aaCGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAacga                                             >  1:107583/1‑149 (MQ=255)
                                                                                                         aaCGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAacga                                             >  3:75718/1‑149 (MQ=255)
                                                                                                             aCTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGaa                                         <  1:257981/149‑1 (MQ=255)
                                                                                                             aCTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGaa                                         <  7:214766/149‑1 (MQ=255)
                                                                                                             aCTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGCAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGaa                                         <  6:226301/149‑1 (MQ=255)
                                                                                                               ttCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAgt                                       >  2:175137/1‑149 (MQ=255)
                                                                                                                 cGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAgtg                                      <  5:150330/148‑1 (MQ=255)
                                                                                                                  gTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTc                                    >  1:213242/1‑149 (MQ=255)
                                                                                                                     aTCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCa                                  <  2:160435/148‑1 (MQ=255)
                                                                                                                      tCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAg                                <  2:75925/149‑1 (MQ=255)
                                                                                                                       cccGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAgg                               <  3:280349/149‑1 (MQ=255)
                                                                                                                         cGGACGAGAAAGCCCGCCTCATCGGTCACGCTACCGCTGAAGTGAAGGAAATCGAGACCCAGTACCCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGACATCGACTTGTGGTCGAAGCCGAACGACGaa                                         <  4:26144/137‑1 (MQ=255)
                                                                                                                         cGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGaa                                         >  3:26144/1‑137 (MQ=255)
                                                                                                                              gagaAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGaa                                         <  5:59777/132‑1 (MQ=255)
                                                                                                                              gagaAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGaa                                         >  6:59777/1‑132 (MQ=255)
                                                                                                                                gaAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAac                                               <  3:200662/124‑1 (MQ=255)
                                                                                                                                gaAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAac                                               >  4:200662/1‑124 (MQ=255)
                                                                                                                                  aaGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGcc                    <  2:24403/149‑1 (MQ=255)
                                                                                                                                     cccGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAAc                 >  1:265878/1‑149 (MQ=255)
                                                                                                                                     cccGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAAc                 <  4:75718/149‑1 (MQ=255)
                                                                                                                                      ccGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAAcc                >  3:129804/1‑149 (MQ=255)
                                                                                                                                       cGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACct               >  8:271286/1‑149 (MQ=255)
                                                                                                                                        gCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACctc              >  3:308253/1‑149 (MQ=255)
                                                                                                                                         cttcatcGGTAGCGTTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACctct             >  4:276585/3‑149 (MQ=255)
                                                                                                                                          ttcatcGGTAGCGTTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAg                                                     >  7:238754/2‑108 (MQ=255)
                                                                                                                                          ttcatcGGTAGCGTTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAg                                                     <  8:238754/107‑1 (MQ=255)
                                                                                                                                            catcGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAgg                                                                                           >  2:196182/1‑68 (MQ=255)
                                                                                                                                              tcGGTAGCGTTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGaaa        <  3:276585/149‑1 (MQ=255)
                                                                                                                                              tcGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGaaa        <  2:265878/149‑1 (MQ=255)
                                                                                                                                                ggTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGACCAAc                 <  7:259540/138‑1 (MQ=255)
                                                                                                                                                ggTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATAGACTTCTGGTCGAAGGCGAACGAAGAAGTGAACAAGGCGATGATGACCAAc                 >  8:259540/1‑138 (MQ=255)
                                                                                                                                                 gTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGaaa        >  7:73783/1‑146 (MQ=255)
                                                                                                                                                   aaCGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTAAGCCTCCGCCCTGGTAACCCAGGGCGAGAACTACAACAAGGTCATCGACTAGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGAAaga      >  1:81035/1‑146 (MQ=255)
                                                                                                                                                    aCGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGAAAGAGaa   <  7:271286/148‑1 (MQ=255)
                                                                                                                                                    aCGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGAAAGAGaa   <  1:126533/148‑1 (MQ=255)
                                                                                                                                                    aCGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGAAAGAGAAg  <  5:61284/149‑1 (MQ=255)
                                                                                                                                                    aCGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGAAAGAGAAg  <  4:274667/149‑1 (MQ=255)
                                                                                                                                                    aCGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGAAAGAGAAg  <  1:175137/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAGTGCTACCGCGTGGTTGGTCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGAAAGAGAAG  >  NC_002947/543579‑543875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑