Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,864,711 C→T 5.9% L480L (CTG→CTA)  cadA‑III ← cadmium translocating P‑type ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,864,7110CT 5.9% 206.3 / 2.2 67L480L (CTG→CTA) cadA‑IIIcadmium translocating P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base C (32/31);  new base T (2/2);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

CCAAGTGGTAGACCTCACCGGCAATGACGCCACGCACGCCACGCCCGGCCAGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTGTCCAGGGCCAGGAAATCAAGGTGGCGGCCACCCTCCAGGTA  >  NC_002947/5864566‑5864851
                                                                                                                                                 |                                                                                                                                            
ccAAGTGGTAGACCTCACCGGCAATGACGCCACGCACTCCACTCCCGGCCAGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTTCTGCTTGCCGAACTCGGCAATGGCGCGGGACAGCGGGTGGTCCGAACGCTCGCCCAgg                                                                                                                                           <  8:108794/149‑1 (MQ=255)
ccAAGTGGTAGACCTCACCGGCAATGACGCCACGCACGCCACGCCCGGCCAGCTCAACGAAGTCACTGACCTCGCTCAAGGCGAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAgg                                                                                                                                           <  6:236654/149‑1 (MQ=255)
 cAAGTGGTAGACCTCACCGGCAATGACGCCACGCACGCCACGCCCGGCCAGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACACGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGc                                                                                                                                          <  1:224865/149‑1 (MQ=255)
        tAGACCTCACCGGCAATGACGCCACGCACGCCACGCCCGGCCAGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGgccgc                                                                                                                                   >  6:179431/1‑149 (MQ=255)
          gACCTCACCGGCAATGACGCCACGCACGCCACGCCCGGCCAGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCa                                                                                                                                             <  8:98454/137‑1 (MQ=255)
          gACCTCACCGGCAATGACGCCACGCACGCCACGCCCGGCCAGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCa                                                                                                                                             >  7:98454/1‑137 (MQ=255)
            ccTCACCGGCAATGACGCCACGCACGCCACGCCCGGCCAGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAg                                                                                                                               <  5:278973/149‑1 (MQ=255)
             cTCACCGGCAATGACGCCACGCACGCCACGCCCGGCCAGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAgg                                                                                                                              <  7:43753/149‑1 (MQ=255)
                 ccGGCAATGACGCCACGCACGCCACGCCCGGCCAGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTg                                                                                                                          <  2:162099/149‑1 (MQ=255)
                  cGGCAATGACGCCACGCACGCCACGCCCGGCCAGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTgc                                                                                                                         >  4:219501/1‑149 (MQ=255)
                          acgccacgcacgccacgcCCGGCCAGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGgcc                                                                                                                                     <  4:174225/129‑1 (MQ=255)
                          acgccacgcacgccacgcCCGGCCAGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGgcc                                                                                                                                     >  3:174225/1‑129 (MQ=255)
                              cacgcacgccacgcCCGGCCAGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTc                                                                                                              >  4:113248/1‑148 (MQ=255)
                                 gcacgccacgcCCGGCCAGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGaa                                                                                                           >  5:287614/1‑148 (MQ=255)
                                    cgccacgccCGGCCAGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAgc                                                                                                       <  3:113248/149‑1 (MQ=255)
                                      ccacgccCGGCCAGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCTAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAgcgg                                                                                                     >  3:180607/1‑149 (MQ=255)
                                      ccacgccCGGCCAGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCTAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAgcgg                                                                                                     >  5:36243/1‑149 (MQ=255)
                                              ggCCAGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCTAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCa                                                                                             <  6:36243/149‑1 (MQ=255)
                                              ggCCAGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCTAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCa                                                                                             <  4:180607/149‑1 (MQ=255)
                                                 cAGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCtt                                                                                                                           >  4:88223/1‑116 (MQ=255)
                                                 cAGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCtt                                                                                                                           <  3:88223/116‑1 (MQ=255)
                                                  aGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAg                                                                                                        >  7:138167/1‑134 (MQ=255)
                                                  aGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAg                                                                                                        <  8:138167/134‑1 (MQ=255)
                                                  aGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCa                                                                                                >  6:188373/1‑142 (MQ=255)
                                                  aGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCa                                                                                                <  5:188373/142‑1 (MQ=255)
                                                     gcAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGc                                                                                      <  1:13519/149‑1 (MQ=255)
                                                            aGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTc                                                                               <  8:116861/149‑1 (MQ=255)
                                                              tCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTg                                                                             >  6:88385/1‑149 (MQ=255)
                                                                aCTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCa                                                                           >  6:94598/1‑149 (MQ=255)
                                                                aCTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCa                                                                           >  8:200438/1‑149 (MQ=255)
                                                                aCTGACCTCGCTCAAGGCCAGGCCATGCTGCTTGCCGAACTCGCCAATGGCGAGGGACAACGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACGTGTGCGTCGGTCTGCa                                                                           >  8:274745/1‑149 (MQ=255)
                                                                    aCCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCgg                                                                       >  6:18864/1‑149 (MQ=255)
                                                                    aCCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCgg                                                                       <  5:94598/149‑1 (MQ=255)
                                                                      cTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCt                                                                     >  3:63134/1‑149 (MQ=255)
                                                                       tCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCtt                                                                    >  8:36637/1‑149 (MQ=255)
                                                                       tCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCtt                                                                    >  1:59065/1‑149 (MQ=255)
                                                                         gCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCtt                                                                    <  8:13797/147‑1 (MQ=255)
                                                                         gCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCtt                                                                    <  8:108254/147‑1 (MQ=255)
                                                                          cTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGcc                                                                 <  7:36637/149‑1 (MQ=255)
                                                                              aggccaggccTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATgg                                                             <  4:63134/149‑1 (MQ=255)
                                                                              aggccaggccTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATgg                                                             <  3:219501/149‑1 (MQ=255)
                                                                                   aggccTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGAt                                                        >  7:27054/1‑149 (MQ=255)
                                                                                     gccTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCAAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATgg                                                      >  1:255494/1‑149 (MQ=255)
                                                                                         tgctgcTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGcc                                                  >  1:263255/1‑149 (MQ=255)
                                                                                              ctTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTc                                              <  2:59065/148‑1 (MQ=255)
                                                                                              ctTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTc                                              <  2:263255/148‑1 (MQ=255)
                                                                                                   cGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTGTc                                         >  3:89487/1‑148 (MQ=255)
                                                                                                    gAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTGTc                                         >  6:234034/1‑147 (MQ=255)
                                                                                                    gAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTGTc                                         >  4:54221/1‑147 (MQ=255)
                                                                                                      aCTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTGTCCAgg                                     <  2:157740/149‑1 (MQ=255)
                                                                                                        tCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTGTCCAGGGc                                   >  8:57882/1‑149 (MQ=255)
                                                                                                        tCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTGTCCAGGGc                                   <  2:255494/149‑1 (MQ=255)
                                                                                                           gCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTGTCCAGGGCCAg                                >  8:75003/1‑149 (MQ=255)
                                                                                                             aaTGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTGTCCAGGGCCAGGa                              <  5:179431/149‑1 (MQ=255)
                                                                                                              aTGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTGTCCAGGGCCAGGaa                             <  7:57882/149‑1 (MQ=255)
                                                                                                              aTGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTGTCCAGGGCCAGGaa                             <  7:75003/149‑1 (MQ=255)
                                                                                                                   gcgGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTGTCCAGGGCCAGGAAATc                          >  2:120873/1‑147 (MQ=255)
                                                                                                                   gcgGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTGTCCAGGGCCAGGAAATc                          >  1:194363/1‑147 (MQ=255)
                                                                                                                      ggACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTGTCCAGGGCCAGGAAATCAAGGt                     <  2:194363/149‑1 (MQ=255)
                                                                                                                           ccGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTGTCCAGGGCCAGGAAATCAAGGTggcgg                >  2:17556/1‑149 (MQ=255)
                                                                                                                                  gtCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTg                                           <  1:236651/115‑1 (MQ=255)
                                                                                                                                  gtCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTg                                           >  2:236651/1‑115 (MQ=255)
                                                                                                                                  gtCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTGTCCAGGGCCAGGAAATCAAGGTggcg                 <  8:124135/141‑1 (MQ=255)
                                                                                                                                  gtCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTGTCCAGGGCCAGGAAATCAAGGTggcg                 >  7:124135/1‑141 (MQ=255)
                                                                                                                                    ccGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTGTCCAGGGCCAGGAAATCAAGGTGGCGGcc              >  5:209335/1‑142 (MQ=255)
                                                                                                                                    ccGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTGTCCAGGGCCAGGAAATCAAGGTGGCGGcc              <  6:209335/142‑1 (MQ=255)
                                                                                                                                          gCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTGTCCAGGGCCAGGAAATCAAGGTGGCGGCCACCCTCCAGGTa  >  8:240703/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
CCAAGTGGTAGACCTCACCGGCAATGACGCCACGCACGCCACGCCCGGCCAGCGCAACGAAGTCACTGACCTCGCTCAAGGCCAGGCCTTGCTGCTTGCCGAACTCGGCAATGGCGCGGGACACCGGGTGGTCCGAACGCTCGCCCAGGCTGGCCGCCAAGGCTTGCGCACGGTCTTCGAACAGCGGCGCCAGCACCTTGGCGTCGGTCTGCACCGGCTTGCCATGGGTGATGGTGCCGGTCTTGTCCAGGGCCAGGAAATCAAGGTGGCGGCCACCCTCCAGGTA  >  NC_002947/5864566‑5864851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑