Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,914,126 C→G 11.4% intergenic (+18/+135) spuI → / ← argA glutamylpolyamine synthetase/amino acid N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,914,1260CG11.4% 170.6 / 10.0 60intergenic (+18/+135)spuI/argAglutamylpolyamine synthetase/amino acid N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (24/29);  new base G (3/4);  total (27/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCC  >  NC_002947/5913985‑5914255
                                                                                                                                             |                                                                                                                                    
ccTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCTTGAGGggcg                                                                                                                               >  1:215373/1‑149 (MQ=255)
ccTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGggcg                                                                                                                               >  6:202003/1‑149 (MQ=255)
ccTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGggcg                                                                                                                               >  2:146506/1‑149 (MQ=255)
 cTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAg                                                                                                                                   >  4:181947/1‑144 (MQ=255)
 cTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAg                                                                                                                                   <  3:181947/144‑1 (MQ=255)
     aCAAGTTCCTCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCGGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCTTCAAGGGCGG‑‑CGtt                                                                                                                        <  8:104675/149‑1 (MQ=255)
     aCAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGtt                                                                                                                        <  8:97218/149‑1 (MQ=255)
      cAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTCCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGttt                                                                                                                       <  5:274579/149‑1 (MQ=255)
        aGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGTTACCTGCATACCGTGTAAACGAAAACGCCGCCCTTGAGGGGCGG‑‑CGttttc                                                                                                                     <  2:215373/149‑2 (MQ=255)
         gTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTtttg                                                                                                                    <  6:42071/149‑1 (MQ=255)
          tACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACAAAAACGCCGCCCTTGAGGGGCGG‑‑CGTTttcgt                                                                                                                   <  4:144567/149‑1 (MQ=39)
              tCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CTTTTTTGTTTga                                                                                                               <  1:6099/149‑1 (MQ=255)
              tCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTga                                                                                                               <  6:174954/149‑1 (MQ=255)
                gATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTgaga                                                                                                             >  8:266130/1‑149 (MQ=255)
                              cGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCg                                                                                                          <  4:69759/138‑1 (MQ=255)
                              cGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCg                                                                                                          >  3:69759/1‑138 (MQ=255)
                               gACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCctc                                                                                               <  5:202003/148‑1 (MQ=255)
                                     ttCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCTTGAGGggcgg‑‑c                                                                                                                           >  2:17647/1‑114 (MQ=255)
                                     ttCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCTTGAGGggcgg‑‑c                                                                                                                           <  1:17647/114‑1 (MQ=255)
                                     ttCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTAGCgg                                                                                        >  1:277662/1‑149 (MQ=255)
                                       cgtcgCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCgg                                                                                        <  5:261843/147‑1 (MQ=255)
                                       cgtcgCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCgg                                                                                        <  7:266130/147‑1 (MQ=255)
                                        gtctCGTGCAAGTAAAGCGAGCTGGAAGAGTTAGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGc                                                                                      <  7:280239/148‑1 (MQ=255)
                                         tcgCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCAc                                                                                    <  8:217047/149‑1 (MQ=255)
                                               gCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGc                                                                              >  8:140419/1‑149 (MQ=255)
                                                 aaGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑Tc                                                                           >  3:260471/1‑149 (MQ=255)
                                                 aaGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑Tc                                                                           >  5:171718/1‑149 (MQ=255)
                                                  aGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCGTGCCCGcgaa                                                                           <  2:185489/149‑4 (MQ=255)
                                                      aaGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCa                                                                      <  7:140419/149‑1 (MQ=255)
                                                         cGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAggg                                                                   >  6:259703/1‑149 (MQ=255)
                                                                   gagTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGaa                                                         <  4:260471/149‑1 (MQ=255)
                                                                     gTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAgg                                                       >  8:96812/1‑149 (MQ=255)
                                                                     cttccaccACTCCAACTCCCACCACCTGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGGCCATTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGaa                                                         <  1:253126/142‑1 (MQ=255)
                                                                         gAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAgg                                                                                                                                  <  1:108815/73‑1 (MQ=255)
                                                                         gAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAgg                                                                                                                                  >  2:108815/1‑73 (MQ=255)
                                                                          aGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAGGCTg                                                    >  5:167622/1‑147 (MQ=255)
                                                                               tCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAGGCTGAAGCCtg                                             >  2:10342/1‑149 (MQ=255)
                                                                               tCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTAGCGGGCCCCCCCCCGACCGGCAGGG‑AATTTAGAAGGGTGGAGccag                                             >  6:286946/1‑147 (MQ=255)
                                                                                 gATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAGGCTGAAGCCtg                                             <  1:146506/147‑1 (MQ=255)
                                                                                       cGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTAc                                     <  6:167622/149‑1 (MQ=255)
                                                                                       cGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑GCCCGCAGGGTAATTTCGAAGGCTGAACCCTGTGACATaa                                     >  5:78435/1‑148 (MQ=255)
                                                                                           cTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCtgt                                 <  7:96812/149‑1 (MQ=255)
                                                                                            tCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCtgtg                                >  4:239086/1‑149 (MQ=255)
                                                                                              gAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTgg                               <  1:10342/148‑1 (MQ=255)
                                                                                                   cAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCgg                         <  1:273068/149‑1 (MQ=255)
                                                                                                   cAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCgg                         <  5:259703/149‑1 (MQ=255)
                                                                                                    aaCTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTATTTTCGAAg                                                        <  7:206273/117‑1 (MQ=255)
                                                                                                    aaCTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAg                                                        >  8:206273/1‑117 (MQ=255)
                                                                                                      cTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGAGACTTACCTGAGGGAGCCGGCg                      >  5:76356/1‑149 (MQ=255)
                                                                                                          tACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTAcc                                    >  4:201434/1‑131 (MQ=255)
                                                                                                          tACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTAcc                                    <  3:201434/131‑1 (MQ=255)
                                                                                                            ccTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGccc                 <  3:239086/148‑1 (MQ=255)
                                                                                                             cTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAGGCTGAAGCCtgt                                            <  3:170251/120‑1 (MQ=255)
                                                                                                             cTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAGGCTGAAGCCtgt                                            >  4:170251/1‑120 (MQ=255)
                                                                                                                  tACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCCCGCCCGC‑TCCCGCAGGGGAATTTGGAAGGCTGGAGCCGGTGCGGTTCCGGTGGGGGCGGGCGTGCCcgcgca            >  1:197194/1‑145 (MQ=255)
                                                                                                                   aCCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAg                                                        >  6:246518/1‑102 (MQ=255)
                                                                                                                   aCCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAg                                                        <  5:246518/102‑1 (MQ=255)
                                                                                                                          aaaCGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGcc  >  5:140588/1‑149 (MQ=255)
                                                                                                                                 aaCGCCGCCCTTGAGGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCtgtg                                >  8:130354/1‑112 (MQ=255)
                                                                                                                                 aaCGCCGCCCTTGAGGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCtgtg                                <  7:130354/112‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                    
CCTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGG‑‑CGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGC‑TCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCC  >  NC_002947/5913985‑5914255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑