Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,923,707	G→T	10.0%	L242M (CTG→ATG) ‡	gcvP‑II ←	glycine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,923,707	0	G	T	10.0%	179.9 / 8.8	60	L242M (CTG→ATG) ‡	gcvP‑II	glycine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (22/32);  new base T (3/3);  total (25/35)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

GCGACAGGCGAAATAGGCCGCGTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCCACAGAATCGACGATCAGCTCAAAGCCGAAGCCTTC  >  NC_002947/5923561‑5923854                                                                                                                                                   |                                                                                                                                                    gcgACAGGCGAAATAGGCCGCGTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAggg                                                                                                                                                   <  7:242532/149‑1 (MQ=255)  cgACAGGCGAAATAGGCCTCGTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGc                                                                                                                                                  <  3:226747/149‑1 (MQ=255)      aGGCGAAATAGGCCGCGTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCctgc                                                                                                                                              >  5:204176/1‑149 (MQ=255)        gCGAAATAGGCCGCGTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCCTGGCctgcg                                                                                                                                             >  2:284788/1‑147 (MQ=255)        gCGAAATAGGCCGCGTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCctgctg                                                                                                                                            <  2:204678/149‑1 (MQ=255)         cGAAATAGGCCGCGTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTgctgg                                                                                                                                           <  2:283275/149‑1 (MQ=255)         cGAAATAGGCCGCGTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTgctgg                                                                                                                                           <  3:192080/149‑1 (MQ=255)           aaaTAGGCCGCGTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTgctggct                                                                                                                                         <  1:117115/149‑1 (MQ=255)            aaTAGGCCGCGTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCCTGGCctgcgtagcc                                                                                                                                        <  1:284788/149‑7 (MQ=255)                ggCCGCGTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGTTGGCTGTGc                                                                                                                                     >  5:212916/1‑148 (MQ=255)                 gCCGCGTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCaa                                                                                                                                   >  7:44750/1‑149 (MQ=255)                       tGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTc                                                                                                                             <  2:82152/149‑1 (MQ=255)                       tGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTc                                                                                                                             <  7:226320/149‑1 (MQ=255)                       tGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTc                                                                                                                             <  8:91532/149‑1 (MQ=255)                                     gCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGgcgc                                                                                                                <  8:44750/148‑1 (MQ=255)                                             gCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCgcgc                                                                                                       >  1:291658/1‑149 (MQ=255)                                              cacaCCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCa                                                                                                      >  3:81166/1‑149 (MQ=255)                                                 acCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACtt                                                                                                   >  7:15482/1‑149 (MQ=255)                                                   cAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCg                                                                                                 >  2:300870/1‑149 (MQ=255)                                                   cAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCg                                                                                                 >  7:179037/1‑149 (MQ=255)                                                    aaaGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGc                                                                                                >  4:146614/1‑149 (MQ=255)                                                         gcTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTgg                                                                                           <  2:291658/149‑1 (MQ=255)                                                         gcTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTgg                                                                                           <  1:300870/149‑1 (MQ=255)                                                                 gAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCgg                                                                                   >  8:45319/1‑149 (MQ=255)                                                                  aGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCggg                                                                                  <  6:204176/149‑1 (MQ=255)                                                                    cccAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTa                                                                                <  8:197125/149‑1 (MQ=255)                                                                       aGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTg                                                                             >  1:181531/1‑149 (MQ=255)                                                                       aGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTg                                                                             >  5:307768/1‑149 (MQ=255)                                                                       aGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTAAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGCTTGACCGGGTACTg                                                                             >  8:170533/1‑149 (MQ=255)                                                                        gCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTgc                                                                            <  8:15482/149‑1 (MQ=255)                                                                                tCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGgc                                                                    <  6:267118/149‑1 (MQ=255)                                                                                  ggCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGgcgc                                                                  <  3:163611/149‑1 (MQ=255)                                                                                  ggCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGgcgc                                                                  <  6:307768/149‑1 (MQ=255)                                                                                            tctcCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACAcc                                                          >  8:254615/1‑147 (MQ=255)                                                                                              tcCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGa                                                        <  7:45319/147‑1 (MQ=255)                                                                                              tcCGGGCGGAGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGt                                                      >  5:430/1‑149 (MQ=255)                                                                                                cGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTcc                                                                                     <  7:156671/116‑1 (MQ=255)                                                                                                cGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTcc                                                                                     >  8:156671/1‑116 (MQ=255)                                                                                                cGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGt                                                      <  7:254615/147‑1 (MQ=255)                                                                                                cGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGt                                                      <  5:222235/147‑1 (MQ=255)                                                                                                       agCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCaa                                             <  6:430/148‑1 (MQ=255)                                                                                                             caccacCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTAt                                       <  8:179037/149‑1 (MQ=255)                                                                                                              accaccAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATc                                      <  6:91008/149‑1 (MQ=255)                                                                                                              accaccAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATc                                      >  5:91008/1‑149 (MQ=255)                                                                                                                 accaGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCca                                    >  7:262952/1‑148 (MQ=255)                                                                                                                    aGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGa                                                        >  5:181095/1‑125 (MQ=255)                                                                                                                    aGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGa                                                        <  6:181095/125‑1 (MQ=255)                                                                                                                          agcagGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCCACAGAATcgac                          <  6:133840/149‑1 (MQ=255)                                                                                                                           gcagGTCAGCGGCTACGCATGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCCACAGAATcgacg                         <  5:267754/149‑1 (MQ=255)                                                                                                                              ggTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCCACAGAATCGACGATc                      <  2:181531/149‑1 (MQ=255)                                                                                                                              ggTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCCACAGAATCGACGATc                      <  8:262952/149‑1 (MQ=255)                                                                                                                                        tACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAgg                                           <  7:23560/118‑1 (MQ=255)                                                                                                                                        tACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAgg                                           >  8:23560/1‑118 (MQ=255)                                                                                                                                        tACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCCACAGAATCGACGATCAGCTCAAAGc            >  6:140703/1‑149 (MQ=255)                                                                                                                                        ccagCAGGCCCTGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCCACAGAATCGACGATCAGc                   <  3:274609/139‑1 (MQ=255)                                                                                                                                        ccagCAGGCCCTGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCCACAGAATCGACGATCAGc                   <  7:195699/139‑1 (MQ=255)                                                                                                                                        ccagCAGGCCCTGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCCACAGAATCGACGATCAGc                   >  8:195699/4‑142 (MQ=255)                                                                                                                                        ccagCAGGCCCTGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGACGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCCACAGAATCGACGATCAGc                   >  4:274609/4‑142 (MQ=255)                                                                                                                                         aCGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACAcc                                                          <  2:281742/102‑1 (MQ=255)                                                                                                                                         aCGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACAcc                                                          >  1:281742/1‑102 (MQ=255)                                                                                                                                                  aGGGCCTGCTGGCTGTGCAAGTTTTCGATCAGCGGTCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCCACAGAATCGACGATCAGCTCAAAGCCGAAGCCTTc  <  7:170533/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GCGACAGGCGAAATAGGCCGCGTGAGGCCCGCCGTAGCCCATCGGCACACCAAAGCGCTGGGTCGAGCCCAGCACCACATCGGCGCCGAGCTCTCCGGGCGGTGCCAGCACCACCAGGCTCAGCAGGTCAGCGGCTACGCAGGCCAGGGCCTGCTGGCTGTGCAACTGGTCGATCAGCGGGCGCAAATCGCGCACTTCGCCGTGGGTGTCCGGGTACTGCAGCAAGGCGCCGAACACCGAGTGCTTGGCAAGGTTATCCACAGAATCGACGATCAGCTCAAAGCCGAAGCCTTC  >  NC_002947/5923561‑5923854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑