Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,954,925 G→A 7.3% G26G (GGC→GGT)  PP_5219 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,954,9250GA 7.3% 159.9 / 2.7 55G26G (GGC→GGT) PP_5219membrane protein
Reads supporting (aligned to +/- strand):  ref base G (26/25);  new base A (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

GGCAATTGCAGCAGCGCCAGGTGCTCGAAGGCCAGAGCATAGGTGACCAGCCCTACGCCTTTGGTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATCCACCAAGATTAGGCCGCACCCACAGGAATCCCTATGG  >  NC_002947/5954785‑5955061
                                                                                                                                             |                                                                                                                                        
ggCAATTGCAGCAGCGCCAGGTGCTCGAAGGCCAGAGCATAGGTGACCAGCCCTACGCCTTTGGTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCAc                                                                                                                                  <  1:172644/149‑1 (MQ=255)
  cAATTGCAGCAGCGCCAGGTGCTCGAAGGCCAGAGCATAGGTGACCAGCCCTACGCCTTTGGTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCAcgc                                                                                                                                >  8:152422/1‑149 (MQ=255)
     ttGCAGCAGCGCCAGGTGCTCGAAGGCCAGAGCATAGGTGACCAGCCCTACGCCTTTGGTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGt                                                                                                                             >  3:183882/1‑149 (MQ=255)
         agcagcGCCAGGTGCTCGAAGGCCAGAGCATAGGTGACCAGCCCTACGCCTTTGGTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGc                                                                                                                         >  4:126225/1‑149 (MQ=255)
           cagcGCCAGGTGCTCGAAGGCCAGAGCATAGGTGACCAGCCCTACGCCTTTGGTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCCGACCGCGTGCGCGGCCAGCgc                                                                                                                       <  1:74983/149‑2 (MQ=255)
            agcGCCAGGTGCTCGAAGGCCAGAGCATAGGTGACCAGCCCTACGCCTTTGGTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGAc                                                                                                                      <  7:210203/149‑1 (MQ=255)
                 cAGGTGCTCGAAGGCCAGAGCATAGGTGACCAGCCCTACGCCGTTGGTCAGCAGGCCGGTAACTGGTCGACAGTGCACCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGc                                                                                                                  >  1:248920/1‑149 (MQ=255)
                      gCTCGAAGGCCAGAGCATAGGTGACCAGCCCTACGCCTTTGGTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGc                                                                                                                                         <  8:11419/120‑1 (MQ=255)
                      gCTCGAAGGCCAGAGCATAGGTGACCAGCCCTACGCCTTTGGTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGc                                                                                                                                         >  7:11419/1‑120 (MQ=255)
                        tCGAAGGCCAGAGCATAGGTGACCAGCCCTACGCCTTTGGTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGc                                                                                                          >  5:81771/1‑149 (MQ=255)
                           aaGGCCAGAGCATAGGTGACCAGCCCTACGCCTTTGGTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTAt                                                                                                       <  8:18238/149‑1 (MQ=255)
                            aGGCCAGAGCATAGGTGACCAGCCCTACGCCTTTGGTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATc                                                                                                      <  5:102034/149‑1 (MQ=255)
                             ggCCAGAGCATAGGTGACCAGCCCTACGCCTTTGGTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCa                                                                                                     <  6:249456/149‑1 (MQ=255)
                              gCCAGAGCATAGGTGACCAGCCCTACGCCTTTGGTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAg                                                                                                    <  7:152422/149‑1 (MQ=255)
                                         ggTGACCAGCCCTACGCCTTTGGTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAg                                                                                          >  2:219050/1‑148 (MQ=255)
                                          gTGACCAGCCCTACGCCTTTGGTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGaa                                                                                        <  6:81771/149‑1 (MQ=255)
                                             aCCAGCCCTACGCCTTTGGTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATgg                                                                                     <  7:14973/149‑1 (MQ=255)
                                                aGCCCTACGCCTTTGGTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTAc                                                                                  >  1:194999/1‑149 (MQ=255)
                                                     tACGCCTTTGGTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCCGACCGCGTGCGCggcca                                                                                                                           >  8:142479/1‑100 (MQ=255)
                                                          cTTTGGTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGa                                                                                                                       >  6:254529/1‑102 (MQ=255)
                                                          cTTTGGTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACGCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGa                                                                                                                       <  5:254529/102‑1 (MQ=255)
                                                               gTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAg                                                                   >  4:306146/1‑149 (MQ=255)
                                                                       gCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGTGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCcagca                                                                          <  4:252460/134‑1 (MQ=255)
                                                                       gCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCcagca                                                                          >  3:252460/1‑134 (MQ=255)
                                                                                  gTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCcagcag                                                                         >  6:296730/1‑125 (MQ=255)
                                                                                  gTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCcagcag                                                                         <  5:296730/125‑1 (MQ=255)
                                                                                  gTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTc                                                 <  1:125104/149‑1 (MQ=255)
                                                                                   tCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCt                                                  >  3:167370/1‑147 (MQ=255)
                                                                                   tCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCt                                                  >  5:283385/1‑147 (MQ=255)
                                                                                     gACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAgg                                              <  2:182194/149‑1 (MQ=255)
                                                                                            cgcCGGTACTCAGGCTGTTGATTGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATc                                       >  2:67503/1‑149 (MQ=255)
                                                                                            cgcCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATc                                       <  3:126225/149‑1 (MQ=255)
                                                                                             accGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTATGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATcc                                      <  2:248920/148‑1 (MQ=255)
                                                                                                  tACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGACCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATccac                                    <  3:32304/146‑1 (MQ=255)
                                                                                                  tACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGACCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATccac                                    >  4:32304/1‑146 (MQ=255)
                                                                                                     tCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATgg                                                           <  7:123867/120‑1 (MQ=255)
                                                                                                     tCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATgg                                                           >  8:123867/1‑120 (MQ=255)
                                                                                                       aGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATCCACCAAGAt                              >  5:109501/1‑147 (MQ=255)
                                                                                                       aGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATCCACCAAGATTa                            <  2:194999/149‑1 (MQ=255)
                                                                                                         gCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATCCACCAAGATTAgg                          <  6:109501/149‑1 (MQ=255)
                                                                                                                aTCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATCCACCAAGATTAGGCCGCAcc                   >  1:134001/1‑149 (MQ=255)
                                                                                                                aTCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATCCACCAAGATTAGGCCGCAcc                   >  4:137863/1‑149 (MQ=255)
                                                                                                                aTCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATCCACCAAGATTAGGCCGCAcc                   >  4:236080/1‑149 (MQ=255)
                                                                                                                   gAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATCCACCAAGATTAGGCCGCACCca                 <  3:236080/148‑1 (MQ=255)
                                                                                                                    aGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATCCACCAAGATTAGGCCGCACCca                 >  3:505/1‑147 (MQ=255)
                                                                                                                        ggCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTATGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATCCACCAAGATTAGGCCGCACCCACAGGaa           >  7:207597/1‑149 (MQ=255)
                                                                                                                         gCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATCCACCAAGATTAGGCCGCACCcaca               >  6:145084/1‑144 (MQ=255)
                                                                                                                         gCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATCCACCAAGATTAGGCCGCACCcaca               <  5:145084/144‑1 (MQ=255)
                                                                                                                         gCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATCCACCAAGATTAGGCCGCACCCACAGGAAt          >  3:259952/1‑149 (MQ=255)
                                                                                                                            aGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATCCACCAAGATTAGGCCGCACCCACAGGAATccc       >  1:317520/1‑149 (MQ=255)
                                                                                                                             gggTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCGCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATCCACCAAGATTAGGCCGCACCCACAGGAATCCCt      <  4:183882/149‑1 (MQ=255)
                                                                                                                                tGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATCCACCAAGATTAGGCCGCACCCACAGGAATCCCTATg   <  4:167370/149‑1 (MQ=255)
                                                                                                                                  aaaTTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATCCACCAAGATTAGGCCGCACCCACAGGAATCCCTATgg  <  1:219050/148‑1 (MQ=255)
                                                                                                                                        cgcTTGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATCCACCAAGATTAGGCCGCACCCACAGGAATCCCTATgg  <  7:124107/142‑1 (MQ=255)
                                                                                                                                        cgcTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATCCACCAAGATTAGGCCGCACCCACAGGAATCCCTATgg  >  8:124107/1‑142 (MQ=255)
                                                                                                                                             |                                                                                                                                        
GGCAATTGCAGCAGCGCCAGGTGCTCGAAGGCCAGAGCATAGGTGACCAGCCCTACGCCTTTGGTCAGCAGGCCGGT‑ACTGGTCGACAGTGCGCCGGTACTCAGGCTGTTGATCGAGTCGGCCAGGGTGAAATTGCGCTGGCCGCGCACGCGGTCGGCGACCAGCTCTACGGCTATCAGCACAAAAAAGAATGGTACGGCCAGCAGTATCAGGTCCATGGGCGCGACCTCAGGCGTTATCCACCAAGATTAGGCCGCACCCACAGGAATCCCTATGG  >  NC_002947/5954785‑5955061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑