Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,970,525 A→G 6.7% W257R (TGG→CGG)  PP_5236 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,970,5250AG 6.7% 258.8 / 6.0 89W257R (TGG→CGG) PP_5236hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (44/39);  new base G (4/2);  total (48/41)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01

GCGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACTGTGGCAG  >  NC_002947/5970380‑5970672
                                                                                                                                                 |                                                                                                                                                   
gCGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCg                                                                                                                                                  <  8:172813/149‑1 (MQ=255)
    cGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTAGACAGCCATCCAGCGCAGCGCATTACGCAACCGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAATGCTGCCGCCCCAGCcggg                                                                                                                                               >  5:63159/1‑148 (MQ=255)
     ggCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCTCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGgca                                                                                                                                             <  5:219839/149‑1 (MQ=255)
       ccTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGgcagc                                                                                                                                           <  4:156162/149‑1 (MQ=255)
       ccTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGgcag                                                                                                                                            >  6:74184/1‑148 (MQ=255)
        cTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCTGCCCCGCTGGGgcggca                                                                                                                                          <  3:261904/149‑1 (MQ=255)
        cTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGgcag                                                                                                                                            >  6:206510/1‑147 (MQ=255)
        cTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGgcag                                                                                                                                            >  2:278010/1‑147 (MQ=255)
           cACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAgg                                                                                                                                        <  3:4410/148‑1 (MQ=255)
             ccTTGTCAGCCTGTGGCAGCTTCAGCCGCCCATCGCCAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCTGGCCATTGACCACCAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTa                                                                                                                                      >  6:108615/1‑148 (MQ=255)
                 gTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTacac                                                                                                                                   <  5:206510/147‑1 (MQ=255)
                  tCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAg                                                                                                                                 >  5:13577/1‑148 (MQ=255)
                      ccTGTGGCAGCTTAAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAgg                                                                                                                            <  1:266740/149‑1 (MQ=255)
                       cTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAggg                                                                                                                           <  8:259084/149‑1 (MQ=255)
                        tgtgGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGc                                                                                                                          >  3:92445/1‑149 (MQ=255)
                        tgtgGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGc                                                                                                                          >  3:80284/1‑149 (MQ=255)
                          tgGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGcc                                                                                                                         <  6:294156/148‑1 (MQ=255)
                            gCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTgg                                                                                                                      <  5:122496/149‑1 (MQ=255)
                             cAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGa                                                                                                                     <  5:74184/149‑1 (MQ=255)
                             cAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGa                                                                                                                     >  6:246341/1‑149 (MQ=255)
                                cTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCg                                                                                                                  <  5:246341/149‑1 (MQ=255)
                                 ttCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTacac                                                                                                                                   <  7:100110/131‑1 (MQ=255)
                                 ttCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTacac                                                                                                                                   >  8:100110/1‑131 (MQ=255)
                                     gCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTc                                                                                                              >  2:302519/1‑148 (MQ=255)
                                     gCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTc                                                                                                              >  3:247276/1‑148 (MQ=255)
                                       cGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCCGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCg                                                                                                             >  8:295494/1‑147 (MQ=255)
                                           cTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAagcag                                                                                                       >  6:15157/1‑149 (MQ=255)
                                           cTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAagcag                                                                                                       >  6:63003/1‑149 (MQ=255)
                                           cTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAagcag                                                                                                       >  6:37397/1‑149 (MQ=255)
                                               gACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCgg                                                                                                   >  6:145794/1‑149 (MQ=255)
                                                aCAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCggg                                                                                                  >  6:164202/1‑149 (MQ=255)
                                                 cAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCgggg                                                                                                 >  5:184474/1‑149 (MQ=255)
                                                  aGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTa                                                                                                                                      <  8:290912/111‑1 (MQ=255)
                                                  aGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTa                                                                                                                                      >  7:290912/1‑111 (MQ=255)
                                                  aGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGgggc                                                                                                <  5:117875/149‑1 (MQ=255)
                                                  aGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGgggc                                                                                                <  6:184474/149‑1 (MQ=255)
                                                  aGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGgggc                                                                                                <  8:205051/149‑1 (MQ=255)
                                                    ccATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGgggcgg                                                                                              <  4:216525/149‑1 (MQ=255)
                                                    ccATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGgggcgg                                                                                              <  1:278010/149‑1 (MQ=255)
                                                         cagcgcagcgCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGAt                                                                                         >  2:254363/1‑149 (MQ=255)
                                                         cagcgcagcgCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCGCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGAt                                                                                         >  6:168509/1‑149 (MQ=255)
                                                             gcagcgcaTTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAgc                                                                                     >  7:35070/1‑149 (MQ=255)
                                                                    aTTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGgc                                                                                                                                              <  1:270784/85‑1 (MQ=255)
                                                                    aTTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGgc                                                                                                                                              >  2:270784/1‑85 (MQ=255)
                                                                         gCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGc                                                                         >  3:55816/1‑149 (MQ=255)
                                                                         gCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGc                                                                         >  3:219828/1‑149 (MQ=255)
                                                                         gCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGc                                                                         <  4:80284/149‑1 (MQ=255)
                                                                         gCAAAAGCTCGTCCCAGGTTGCGCAAAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGTGCCTGGATCGCTTCGAAGCAGCAGGGGCGGGAGATCAGCGCATGGCGCAGc                                                                         >  6:108680/1‑149 (MQ=255)
                                                                                   gTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATa                                                                 <  5:37397/147‑1 (MQ=255)
                                                                                      ccAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAg                                                            <  6:13577/149‑1 (MQ=255)
                                                                                       cAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAgg                                                           >  6:146288/1‑149 (MQ=255)
                                                                                               gcACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGt                                                   >  8:80802/1‑149 (MQ=255)
                                                                                               gcACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGt                                                   <  4:92445/149‑1 (MQ=255)
                                                                                                cagaGCGTGCCATTGTCCAACAGCTCGAACGCATCAAGGCTGCGGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGtt                                                  <  5:108615/146‑1 (MQ=255)
                                                                                                   aGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTc                                               <  5:164202/149‑1 (MQ=255)
                                                                                                      gggCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATg                                            <  4:55816/149‑1 (MQ=255)
                                                                                                      gggCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATg                                            <  7:80802/149‑1 (MQ=255)
                                                                                                                ccAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGc                                  >  2:105794/1‑149 (MQ=255)
                                                                                                                 cAACAGCTCGAACGCATCAAGGCTGCCTCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAgcgc                                                                                   <  5:294431/99‑1 (MQ=255)
                                                                                                                 cAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCt                                 >  6:281739/1‑149 (MQ=255)
                                                                                                                 cAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAACCAGCCGGGGCGGGCGATCAgcgc                                                                                   >  6:294431/1‑99 (MQ=255)
                                                                                                                    cAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGc                              <  5:51539/149‑1 (MQ=255)
                                                                                                                     aGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGcc                             <  7:295494/149‑1 (MQ=255)
                                                                                                                      gCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCt                                                                                                                <  6:229679/65‑1 (MQ=255)
                                                                                                                      gCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCt                                                                                                                >  5:229679/1‑65 (MQ=255)
                                                                                                                       cTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGcc                             >  4:5105/1‑147 (MQ=255)
                                                                                                                        tCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGcc                             >  6:313081/1‑146 (MQ=255)
                                                                                                                          gAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGCTCAGCGCATGGCGCAGCCAGTAATAGCGAGCGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGa                         >  5:250833/1‑148 (MQ=255)
                                                                                                                           aaCGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATa                       <  5:145794/149‑1 (MQ=255)
                                                                                                                            aCGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTg                               <  5:160012/140‑1 (MQ=255)
                                                                                                                            aCGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTg                               >  6:160012/1‑140 (MQ=255)
                                                                                                                                aTCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTg                               <  5:147451/136‑1 (MQ=255)
                                                                                                                                aTCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTg                               >  6:147451/1‑136 (MQ=255)
                                                                                                                                aTCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGcgcg                  >  6:207031/1‑149 (MQ=255)
                                                                                                                                aTCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGcgcg                  >  2:78204/1‑149 (MQ=255)
                                                                                                                                   aaGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCa               <  1:254363/149‑1 (MQ=255)
                                                                                                                                    tacctgctgCCCCGCTGGGGCGGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTc                                                     >  5:308766/4‑110 (MQ=255)
                                                                                                                                    tacctgctgCCCCGCTGGGGCGGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAg              >  3:34801/4‑149 (MQ=255)
                                                                                                                                    tacctgctgCCCCGCTGGGGCGGCAGGTACACAGCAAGCGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTc                                                     <  6:308766/107‑1 (MQ=255)
                                                                                                                                       ctgctgCCCCGCTGGGGCGGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCg            >  8:108945/1‑148 (MQ=255)
                                                                                                                                       ctgctgCCCCGCTGGGGCGGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGa           >  5:61691/1‑149 (MQ=255)
                                                                                                                                        tGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGTGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGAc          >  6:285734/1‑149 (MQ=255)
                                                                                                                                           cgccCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCa                                              <  4:140713/110‑1 (MQ=255)
                                                                                                                                           cgccCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCa                                              >  3:140713/1‑110 (MQ=255)
                                                                                                                                           cgccCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACtgt       <  5:63003/149‑1 (MQ=255)
                                                                                                                                             ccccAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACTGTgg     <  5:146288/149‑1 (MQ=255)
                                                                                                                                              cccAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACTGTGGc    <  6:272973/149‑1 (MQ=255)
                                                                                                                                               ccAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACTGTgg     >  3:206638/1‑147 (MQ=255)
                                                                                                                                                cAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACTGTGGCAg  >  1:217089/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACTGTGGCAG  >  NC_002947/5970380‑5970672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑