Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,975,678 T→A 10.6% intergenic (+622/‑522) PP_5727 → / → yifB hypothetical protein/ATP‑dependent chelatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,975,6780TA10.6% 118.5 / 5.5 47intergenic (+622/‑522)PP_5727/yifBhypothetical protein/ATP‑dependent chelatase
Reads supporting (aligned to +/- strand):  ref base T (17/25);  new base A (3/2);  total (20/27)
Fisher's exact test for biased strand distribution p-value = 6.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.82e-01

TTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGATCATCAGACTTGAGCACGAGTGCCATAGCAGAAGCTAGCTTGCGACTGATGGGGCTGTGCTCCGGTACCGAAACGCATTAACCAGAT  >  NC_002947/5975533‑5975825
                                                                                                                                                   |                                                                                                                                                   
tttGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATaa                                                                                                                                                   <  5:58577/148‑1 (MQ=255)
tttGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATaa                                                                                                                                                   <  6:159445/148‑1 (MQ=255)
      aaaaTAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAGAAGTGAGGAATTATAAATATTGAACCTGGAAAAGATACGATGCGTATAAGCGGGCCATACGAATACCTGCAAAACATATATAatattt                                                                                                                                              >  1:98434/1‑147 (MQ=255)
       aaaTAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATAtttt                                                                                                                                           <  2:236487/149‑1 (MQ=255)
               gCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTaa                                                                                                                                   <  2:225320/149‑1 (MQ=255)
                cGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAg                                                                                                                                  <  6:65608/149‑1 (MQ=255)
                 ggaggaTTCTCGAGAGAGTAAAATAAATTCAGCATGCTCCATCAATTCAAAAGTGA‑‑AATTATACATATTGAACCTGGAAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGt                                                                                                                                 <  3:3907/149‑1 (MQ=255)
                 ggaggaTTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGt                                                                                                                                 <  1:104670/149‑1 (MQ=255)
                     gaTTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCt                                                                                                                             >  4:301011/1‑149 (MQ=255)
                            gagagaGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGt                                                                                                                       <  7:141026/148‑1 (MQ=255)
                            gagagaGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTg                                                                                                                      >  2:198260/1‑149 (MQ=255)
                             agagagGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTgg                                                                                                                     <  2:120732/149‑1 (MQ=255)
                                  ggAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAATATCTTATAGATGTTTTGCAgg                                                                                                                                      >  6:199581/1‑126 (MQ=255)
                                  ggAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAATATCTTATAGATGTTTTGCAgg                                                                                                                                      <  5:199581/127‑2 (MQ=255)
                                  ggAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTa                                                                                                                <  2:157339/149‑1 (MQ=255)
                                  ggAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTa                                                                                                                >  3:73641/1‑149 (MQ=255)
                                      aataaatTCAGCATGCTCCATCAATGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTAcaca                                                                                                            <  1:198260/149‑1 (MQ=255)
                                      aataaatTCAGCATGCTCCATCAATGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTAcaca                                                                                                            >  3:176782/1‑149 (MQ=255)
                                            ttCAGCATGCTCCATCAATGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATAtttt                                                                                                                                           >  4:201276/1‑112 (MQ=255)
                                            ttCAGCATGCTCCATCAATGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATAtttt                                                                                                                                           <  3:201276/112‑1 (MQ=255)
                                                gCATGCTCCATCAATGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGcc                                                                                                  >  8:65769/1‑149 (MQ=255)
                                                  aTGCTCCATCAATGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCg                                                                                                 >  6:97750/1‑148 (MQ=255)
                                                            aaTGCACAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATCCGTATAAGCGGGCCATAAGAAGACCTGCAAAACATATATAAGATATTTTGAATTTTAGTGCCTCGTAGTGGCTCTACACAGAAAAGGGCCGAATTTTGatc                                                                                       <  2:98434/148‑1 (MQ=255)
                                                              tGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGatcatc                                                                                    <  4:176782/149‑1 (MQ=255)
                                                               gCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGatcat                                                                                     >  2:250155/1‑147 (MQ=255)
                                                                                  cATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGATCATCAGACTTGAGCACGAGTg                                                                   <  3:45394/148‑1 (MQ=255)
                                                                                     atTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGATCATCAGACTTGAACACGAGTGCCa                                                                >  6:7190/1‑148 (MQ=255)
                                                                                             tGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGatcatc                                                                                    >  8:157260/1‑120 (MQ=255)
                                                                                             tGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGatcatc                                                                                    <  7:157260/120‑1 (MQ=255)
                                                                                               gCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGATCATCAGACTTGAGCACGAGTGCCATAGCAGAagct                                                     <  1:250155/149‑1 (MQ=255)
                                                                                               gCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGATCATCAGACTTGAGCACGAGTGCCATAGCAGAagc                                                      >  5:275016/1‑148 (MQ=255)
                                                                                                 aaaGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGATCATCAGACTTGAGCACGAGTGCCATAGCAGAagctag                                                   <  3:301011/149‑1 (MQ=255)
                                                                                                         gATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTg                                                                                                                                          <  8:284870/54‑1 (MQ=255)
                                                                                                         gATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTg                                                                                                                                          >  7:284870/1‑54 (MQ=255)
                                                                                                          aTGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGATCATCAGACTTGAGCACGAGTGCCATAGCAGAAGCTAGCTTGCGACt                                          >  6:17155/1‑149 (MQ=255)
                                                                                                             cGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGATCATCAGACTTGAGCACGAGTGCCATAGCAGAAGCTAGCTTGCGACTGAt                                       <  5:97750/149‑1 (MQ=255)
                                                                                                             cGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGATCATCAGACTTGAACACGAGTGCCATAGCAGAAGCTAGCTTGCGACTGAt                                       <  5:7190/149‑1 (MQ=255)
                                                                                                                     cGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGATCATCAGACTTGAGCACGAGTGCCATAGCAGAAGCTAGCTTGCGACTGATGGGGCtgt                               <  6:275016/149‑1 (MQ=255)
                                                                                                                        gCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGATCATCAGACTTGAGCACGAGTGCCATAGCAGAAGCTAGCTTGCGACTGATGGGGCtgtg                              >  3:258801/1‑147 (MQ=255)
                                                                                                                        gCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGATCATCAGACTTGAGCACGAGTGCCATAGCAGAAGCTAGCTTGCGACTGATGGGGCtgtg                              <  4:258801/147‑1 (MQ=255)
                                                                                                                         ccATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGATCATCAGACTTGAGCACGAGTGCCATAGCAGAAGCTAGCTTGCGACTGATGGGGCTGTGCTc                           >  5:234379/1‑149 (MQ=255)
                                                                                                                             aCGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGATCATCAGACTTGAGCACGAGTGCCATAGCAGAAGCTAGCTTGCGACTGATGGGGCTGTGCTCCgg                        >  8:148530/1‑148 (MQ=255)
                                                                                                                                 ttaacTGCAAAATATCTTATAGATGTTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGATCATCAGACTTGAGCACGAGTGCCATAGCAGAagcta                                                    >  1:70729/5‑116 (MQ=38)
                                                                                                                                 ttaacTGCAAAATATCTTATAGATGTTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGATCATCAGACTTGAGCACGAGTGCCATAGCAGAagcta                                                    <  2:70729/112‑1 (MQ=38)
                                                                                                                                      tGCAAAATATCTTATAGATGTTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGATCATCAGACTTGAGCACGAGTGCCATAGCAGAAGCTAGCTTGCGACTGATGGGGCTGTGCTCCGGTACCGAAACg              >  5:280327/1‑149 (MQ=255)
                                                                                                                                           aaCATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGATCATCAGACTTGAGCACGAGTGCCATAGCAGAAGCTAGCTTGCGACTGATGGGGCTGTGCTCCGGTACCGAAACGCATTa         <  7:65769/149‑1 (MQ=255)
                                                                                                                                            aCATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGATCATCAGACTTGAGCACGAGTGCCATAGCAGAAGCTAGCTTGCGACTGATGGGGCTGTGCTCCGGTACCGAAACGCATTaa        <  7:148530/149‑1 (MQ=255)
                                                                                                                                                  ataAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGATCATCAGACTTGAGCACGAGTGCCATAGCAGAAGCTAGCTTGCGACTGATGGGGCTGTGCTCCGGTACCGAAACGCATTAACCAGAt  >  7:267000/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGA‑‑AATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTACACAGAAAAGGGCCGAATTTTGATCATCAGACTTGAGCACGAGTGCCATAGCAGAAGCTAGCTTGCGACTGATGGGGCTGTGCTCCGGTACCGAAACGCATTAACCAGAT  >  NC_002947/5975533‑5975825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑