Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,981,889	A→C	6.4%	Q845P (CAG→CCG)	PP_5242 →	GAF domain‑containing sensor histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,981,889	0	A	C	6.4%	200.2 / 4.6	63	Q845P (CAG→CCG)	PP_5242	GAF domain‑containing sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base A (27/32);  new base C (2/2);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

CGCTCGGTGTTGGACCAGGCACGGGCGTGCCGGCTGCAGGCGCTGCTGTACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGACTCGGAGGAAGTGACGGTTGCCATCAGCCTGCTGGCCACTCTGTCATCATCCTTCTTCGTGAAGGA  >  NC_002947/5981744‑5982036                                                                                                                                                  |                                                                                                                                                      cgctcgGTGTTGGACCAGGCACGGGCGTGCCGGCTGCAGGCGCTGCTGTACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGt                                                                                                                                                    >  8:58287/1‑149 (MQ=255)  gctcgGTGTTGGACCAGGCACGGGCGTGCCGGCTGCAGGCGCTGCTGTACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTc                                                                                                                                                   <  8:49249/149‑1 (MQ=255)  gctcgGTGTTGGACCAGGCACGGGCGTGCCGGCTGCAGGCGCTGCTGTACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTc                                                                                                                                                   >  8:209552/1‑149 (MQ=255)      ggtggtGGACCAGGCACGGGCGTGCCGGCTGCAGGCGCTGCTGTACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACCTGCTGCCAGTCGAgg                                                                                                                                               <  6:327133/149‑1 (MQ=255)      ggTGTTGGACCAGGCACGGGCGTGCCGGCTGCAGGCGCTGCTGTACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAgg                                                                                                                                               >  1:121107/1‑149 (MQ=255)       gtgtTGGACCAGGCACGGGCGTGCCGGCTGCAGGCGCTGCTGTACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGc                                                                                                                                              >  4:218653/1‑149 (MQ=255)       gtgtTGGACCAGGCACGGGCGTGCCGGCTGCAGGCGCTGCTGTACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACCTGCTGCCAGTCGAGGc                                                                                                                                              <  2:184209/149‑1 (MQ=255)       gtgtTGGACCAGGCACGGGCGTGCCGGCTGCAGGCGCTGCTGTACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGCTAGCAGCCCGGTGCGGGGCCTCGACTCGCAGCAGGTCGAGGc                                                                                                                                              <  8:119891/149‑1 (MQ=255)       gtgtTGGACCAGGCACGGGCGTGCCGGCTGCAGGCGCTGCTGAACAACTTGCGCGGTGACTATCAGACGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGCGCCTCGACTGGCACCAGGTCGAGGc                                                                                                                                              >  3:152180/1‑149 (MQ=255)         gtTGGACCAGGCACGGGCGTGCCGGCTGCAGGCGCTGCTGTACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGc                                                                                                                                              >  5:146339/1‑147 (MQ=255)             gACCAGGCACGGGCGTGCCGGCTGCAGGCGCTGCTGTACACCTTGCGCGGTGACTTTCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAg                                                                                                                                                >  6:65310/1‑141 (MQ=255)             gACCAGGCACGGGCGTGCCGGCTGCAGGCGCTGCTGTACACCTTGCGCGGTGACTTTCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAg                                                                                                                                                <  5:65310/141‑1 (MQ=255)             gACCAGGCACGGGCGTGCCGGCTGCAGGCGCTGCTGTACACCGTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCa                                                                                                                                        >  1:61611/1‑149 (MQ=255)               ccAGGCACGGGCGTGCCGGCTGCAGGCGATGCTGTACACCTTGCGCGGTGACTATCAGACGGGCATCGACAGCGCGCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATg                                                                                                                                      <  4:152180/149‑1 (MQ=255)                cAGGCACGGGCGTGCCGGCTGCAGGCGCTGCTGTACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGc                                                                                                                                     <  1:137144/149‑1 (MQ=255)                  ggCACGGGCGTGCCGGCTGCAGGCGCTGCTGTACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGcc                                                                                                                                    >  3:147974/1‑148 (MQ=255)                          cGTGCCGGCTGCAGGCGCTGCTGTACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑g                                                                                                                         <  4:131074/149‑1 (MQ=255)                                  cTGCAGGCGCTGCTGTACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGAt                                                                                                                 <  3:218653/149‑1 (MQ=255)                                   agCAGGCCATGCTGTACACCATGCGCCTAGACTATCAGGCGGCCATCGACAGCGCCCTGTCCGGCCTCGACTTACTGGGTATCAGCCCGGTGCGGGCCCTCGACTGCCACCAGGTCTAGGCCAGTCATGCA‑CATGTTCA‑GGCGTTGCTc                                                                                                                >  8:154997/2‑149 (MQ=255)                                     cAGGCGCTGCTGTACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATc                                                                                                                <  6:146339/147‑1 (MQ=255)                                      aGGCGCTGCTGTACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGaa                                                                                                             <  4:188217/149‑1 (MQ=255)                                       ggCGCTGCTGTACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAAc                                                                                                            <  2:264078/149‑1 (MQ=255)                                        gcgcTGCTGTACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACa                                                                                                           <  4:147974/149‑1 (MQ=255)                                               tgTACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGAc                                                                                                    >  5:58909/1‑149 (MQ=255)                                                gTACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGAcc                                                                                                   >  8:255868/1‑149 (MQ=255)                                                 tACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCg                                                                                                  >  5:18976/1‑149 (MQ=255)                                                 tACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCg                                                                                                  <  6:58909/149‑1 (MQ=255)                                                   cacCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCgg                                                                                                 <  7:209552/148‑1 (MQ=255)                                                    acCTTGCGCGGTGACTTTCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGc                                                                                                                       <  1:307795/125‑1 (MQ=255)                                                    acCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGc                                                                                                                       >  2:307795/1‑125 (MQ=255)                                                      cTTGCGCGGTGACTATCAGGCTGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCAtt                                                                                             <  7:154997/149‑1 (MQ=255)                                                      cTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCAtt                                                                                             >  4:93006/1‑149 (MQ=255)                                                           gcgGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTg                                                                                        >  8:10529/1‑149 (MQ=255)                                                           gcgGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTg                                                                                        <  7:197/149‑1 (MQ=255)                                                           gcgGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTg                                                                                        >  8:195573/1‑149 (MQ=255)                                                             ggTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGa                                                                                      <  7:255868/149‑1 (MQ=255)                                                             ggTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGa                                                                                      <  6:18976/149‑1 (MQ=255)                                                             ggTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGa                                                                                      <  8:278237/149‑1 (MQ=255)                                                                             ccATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGa                                                                                      >  1:47584/1‑133 (MQ=255)                                                                             ccATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGa                                                                                      <  2:47584/133‑1 (MQ=255)                                                                                tCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGTGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGa                                                                    >  6:83596/1‑148 (MQ=255)                                                                                tCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGAc                                                                   <  7:195573/149‑1 (MQ=255)                                                                                   aCAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGACTCg                                                                >  2:66684/1‑149 (MQ=255)                                                                                     aGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGACTCgga                                                              <  2:121107/149‑1 (MQ=255)                                                                                     aGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGACTCgga                                                              <  4:207956/149‑1 (MQ=255)                                                                                      gcgcCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGACTCggag                                                             <  5:43494/149‑1 (MQ=255)                                                                                                cggccTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGACTCGGAGGAAGTGACgg                                                   >  7:8066/1‑149 (MQ=255)                                                                                                  gccTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGACTCGGAGGAAGTGACGGtt                                                 <  2:61611/149‑1 (MQ=255)                                                                                                    cTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGACTCGGAGGAAGTGACGGTTGc                                               >  1:119112/1‑149 (MQ=255)                                                                                                    cTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGACTCGGAGGAAGTGACGGTTGc                                               <  2:119112/149‑1 (MQ=255)                                                                                                        aCTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGTGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGACTCGGAGGAAGTGACGGTTGCCATc                                           <  5:83596/149‑1 (MQ=255)                                                                                                            aCTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGa                                                                                                                  >  6:260980/1‑74 (MQ=255)                                                                                                            aCTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGa                                                                                                                  <  5:260980/74‑1 (MQ=255)                                                                                                              tGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCAGGGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGACTCGGAGGAAGTGACGGTTGCCATCAGCct                                      >  1:253297/1‑149 (MQ=255)                                                                                                                ggTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCCGCATGTGAA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGACACTGCCGCGCCCCGAAACGGAGGAAGTGACGTTTGCCATCACCctgc                                    >  1:82700/1‑149 (MQ=255)                                                                                                                        cccGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGACTCGGAGGAAGTGACGGTTGCCATCAGCCTGCTGGCCAc                             <  5:162041/147‑1 (MQ=255)                                                                                                                        cccGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGACTCGGAGGAAGTGACGGTTGCCATCAGCCTGCTGGCCAc                             >  6:162041/1‑147 (MQ=255)                                                                                                                            gTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGACTCGGAGGAAGTGACGGTTGCCATCAGCCTGCTGGCCACTCTGtc                       <  3:93006/149‑1 (MQ=255)                                                                                                                            gTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGACTCGGAGGAAGTGACGGTTGCCATCAGCCTGCTGGCCACTCTGt                        >  1:247381/1‑148 (MQ=255)                                                                                                                                ggggCCTCGACTGGGAGGAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGATGGTCAGGCATTGCCTGGATTCACTGCCGCGCACCGACTCGGAGGAAGTGACGGTTGCCATCAGCCTGCTGGCCACTCTGtcatca                   <  2:111032/149‑1 (MQ=255)                                                                                                                                    ccTCGACCTGCTGCCAGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGACTCGGAGGAAGTGACGGTTGCCATCAGCCTGCTGGCCACTCTGTCATCATCct               >  7:100915/1‑149 (MQ=255)                                                                                                                                    ccTCGACCTGCTGCCAGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGACTCGGAGGAAGTGACGGTTGCCATCAGCCTGCTGGCCACTCTGTCATCATCct               >  8:43287/1‑149 (MQ=255)                                                                                                                                         aCTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGACTCGGAGGAAGTGACGGTTGCCATCAGCCTGCTGGCCACTCTGTCATCATCCttcttc          <  4:306872/149‑1 (MQ=255)                                                                                                                                             gcagcaGGTCGAGCCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCAATGCCTGGAGTCACTGCCGCGCACCGACTCGGACGAAGTGAAGGTTGCCATCAGCCTGCTGGACACTCTCTCATCATCCTTCATCGTg       >  8:265966/1‑148 (MQ=255)                                                                                                                                                 cagGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGACTCGGAGGAAGTGACGGTTGCCATCAGCCTGCTGGCCACTCTGTCATCATCCTTCTTCGTGAAGga  <  1:66684/149‑1 (MQ=255)                                                                                                                                                  agGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGACTCGGAGGAAGTGACGGTTGCCATCAGCCTGCTGGc                                <  5:175496/118‑1 (MQ=255)                                                                                                                                                  agGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGACTCGGAGGAAGTGACGGTTGCCATCAGCCTGCTGGc                                >  6:175496/1‑118 (MQ=255)                                                                                                                                                  |                                                                                                                                                      CGCTCGGTGTTGGACCAGGCACGGGCGTGCCGGCTGCAGGCGCTGCTGTACACCTTGCGCGGTGACTATCAGGCGGCCATCGACAGCGCCCTGGCCGGCCTGGACTTACTGGGTATCAGCCCGGTGCGGGGCCTCGACTGGCAGCAGGTCGAGGCCAGTCATGCC‑CATGTGCA‑GGCGTTGATCGAACAGAAGGACCGGCATTGCCTGGAGTCACTGCCGCGCACCGACTCGGAGGAAGTGACGGTTGCCATCAGCCTGCTGGCCACTCTGTCATCATCCTTCTTCGTGAAGGA  >  NC_002947/5981744‑5982036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑