| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,004,968 | T→C | 7.5% | intergenic (+6/‑170) | PP_5259 → / → ydcJ | LysR family transcriptional regulator/metalloprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,004,968 | 0 | T | C | 7.5% | 150.4 / 2.7 | 53 | intergenic (+6/‑170) | PP_5259/ydcJ | LysR family transcriptional regulator/metalloprotein |
| Reads supporting (aligned to +/- strand): ref base T (23/26); new base C (2/2); total (25/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01 | |||||||||||
TGGCCGACGGTAGCCTGGTGGTGCCGTGGAACCATGCAATGCGCAGTGCCGGAGCGCA‑TTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGT > NC_002947/6004820‑6005107 | tGGCCGACGGTAGCCTGGTGGTGCCGTGGAACCATGCAATGCGCAGTGCCGGAGCGCA‑TTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCct > 7:122266/1‑149 (MQ=255) gCCGACGGTAGCCTGGTGGTGCCGGGGAACCATGCAATGCGCAGTGCCGGAGCGCA‑TTACCTGGCGTATGCCGAGCATGCGGAAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGCGTGGCGAGCAGTTGCAGGTTTGACGGCctct > 2:75527/1‑149 (MQ=255) ggTAGCCTGGTGGTGCCGTGGAACCATGCAATGCGCAGTGCCGGAGCGCA‑TTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCgg < 4:189712/149‑1 (MQ=255) ccTGGTGGTGCCGTGGAACCATGCAATGCGCAGTGCCGGAGCGCACTTAACTGCCCAATGCCGATCATGCGGCAGAGGTGCCAAAGCTGCGGGCGCTGGTCGAATGGATTGGCGAGCAGTTGCAGGATTGACGGCCTCTTCGCggatt > 1:225150/1‑145 (MQ=255) ccTGGTGGTGCCGTGGAACCATGCAATGCGCAGTGCCGGAGCGCA‑TTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTaaa < 5:73738/149‑1 (MQ=255) cTGGTGGTGCCGTGGAACCATGCAATGCGCAGTGCCGGAGCGCA‑TTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAg > 4:264344/1‑149 (MQ=255) gCCGTGGAACCATGCAATGCGCAGTGCCGGAGCGCA‑TTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTcc > 1:277017/1‑148 (MQ=255) ccGTGGAACGATGCAATGCGCAGTGGCGGAGCGCA‑TTACCTGGCGTATGCCGAGCATGCGTCAGAGGTGCCATAGGTGGGGGCGCTGGTTGAATGGATTGGCGAGCTGTTGGAGGTTTGACGGCCTCTTCGCGGCTTTAGCCGcgaaga < 2:225150/149‑6 (MQ=255) gTGGAACCATGCAATGCGCAGTGCCGGAGCGCA‑TTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCCTTTCCCGGCTAAAGCCGCGCCTACg > 4:197700/1‑149 (MQ=255) tGGAACCATGCAATGCGCAGTGCCGGAGCGCA‑TTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGAGGGCGCTGGTGGAATGGATTGGCGAGCAGGTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTACTACgg > 5:237482/1‑149 (MQ=255) aCCATGCAATGCGCAGTGCCGGAGCGCA‑TTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGAc > 6:105077/1‑149 (MQ=255) ccATGCAATGCGCAGTGCCGGAGCGCA‑TTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGAcc < 3:264344/149‑1 (MQ=255) cATGCAATGCGCAGTGCCGGAGCGCA‑TTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACcg > 6:165044/1‑149 (MQ=255) cATGCAATGCGCAGTGCCGGAGCGCA‑TTACCTGGCGTATGCCGAGCATGCCGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTAGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACcg > 1:29320/1‑149 (MQ=255) aTGCAATGCGCAGTGCCGGAGCGCA‑TTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAg > 7:186463/1‑130 (MQ=255) aTGCAATGCGCAGTGCCGGAGCGCA‑TTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAg < 8:186463/130‑1 (MQ=255) cAATGCGCAGTGCCGGAGCGCA‑TTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTa < 5:105077/149‑1 (MQ=255) cgcAGTGGCGGTGCGGA‑TTACCTGGCGTATGCCGTGGATGGGGCAGAGGTGCCAATGGTGCGTGCGCTGGTGGAATGGATTGGCGAGGAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGgcgc < 2:88086/149‑1 (MQ=255) tGCCGGAGCGCA‑TTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGTGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCGCCTACGCGGTCCCCGTAGGAGCGGCtt < 8:3829/149‑1 (MQ=38) ccGGAGCGCA‑TTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCtt > 8:265570/1‑147 (MQ=255) gcgcA‑TTACCTGGCGTATGCCGAGCCTGCGGCAGAGGTGCCAACGGTGCGGGCGCTGGTGGAATGGATTGGCTAGCAGTTGCAGGGTTGACGGCCTCTTCGCGGCTAACGCCGCTCCTCCGGTGACCGCGTAGGCGCGGCTTTAGCCgg < 1:75527/149‑1 (MQ=255) gcgcA‑TTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCCTTTCCCGGCTAAAGCCGCGCCTACGCGGTCCCCGTAGGAGCGGCTTTAGCCgc < 3:197700/149‑2 (MQ=25) cA‑TTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGa > 5:315157/1‑148 (MQ=255) aCCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑g‑g < 2:277017/148‑1 (MQ=255) cTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCCCGGCTAAAGCCGCGCCTACGCGGTCCCCGTAGGAGCGGCTTTAGCCGCGaa > 8:245175/1‑143 (MQ=25) cTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCCCGGCTAAAGCCGCGCCTACGCGGTCCCCGTAGGAGCGGCTTTAGCCGCGaa < 7:245175/143‑1 (MQ=25) cTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGGTGCAGGTTTGCCGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGGAGGCGGGGCGTTAGGCGGGAAC‑G‑GGcc > 8:209357/1‑149 (MQ=39) gCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTc > 5:121852/1‑149 (MQ=255) aGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATc < 4:74850/149‑1 (MQ=255) gCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATCAAAAAAg > 6:281208/1‑148 (MQ=255) gagGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATCAAAAAAGGAAt < 3:226510/149‑1 (MQ=255) ggTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATCAAAAAAGGAATGa < 6:315157/149‑1 (MQ=255) gTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATCAAAAAAGGAATGac < 7:265570/149‑1 (MQ=255) ccAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATCAAAAAAGGAATGacaca < 7:53422/149‑1 (MQ=255) cAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCCTTTCCCGGCTAAAGCCGCGCCTACGCGGTCCCCGTAGGAGCGGCTTTAGCCGCGAAG‑A‑GGCCg > 6:96795/1‑119 (MQ=14) cAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCCTTTCCCGGCTAAAGCCGCGCCTACGCGGTCCCCGTAGGAGCGGCTTTAGCCGCGAAG‑A‑GGCCg < 5:96795/119‑1 (MQ=14) ggCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTAGCGGCTAAAGCCGCTCCTACGGGGAc < 3:102904/76‑1 (MQ=255) ggCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTAGCGGCTAAAGCCGCTCCTACGGGGAc > 4:102904/1‑76 (MQ=255) gcTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCg > 2:235410/1‑149 (MQ=255) gtggAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCtt < 6:121852/148‑1 (MQ=255) tggAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTtgt < 6:267417/149‑1 (MQ=255) gcaTGGCTTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGa < 2:282786/147‑1 (MQ=255) gAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGa < 1:235410/149‑1 (MQ=255) aaTGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAg > 2:194581/1‑149 (MQ=255) aTGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGc < 4:94530/141‑1 (MQ=255) aTGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGc > 3:94530/1‑141 (MQ=255) aggtGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCa < 8:122266/146‑1 (MQ=255) gCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCt > 6:229536/1‑148 (MQ=255) gCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCt < 5:229536/148‑1 (MQ=255) gCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCGGCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGTAA‑G‑GGGCGTCGAGGTCGATCAAAAAAGGGATGACCCACTCACTTTTTTTGGGTGGTGAGCAAAGGGCGTTCCCCGCtt > 5:185034/1‑149 (MQ=255) gTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAg < 2:206198/142‑1 (MQ=255) gTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAg > 1:206198/1‑142 (MQ=255) gTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCAt > 3:222370/1‑149 (MQ=255) tttGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAATGTGGCGGGGGAGGGCGATCAAAAAAGGAATGACACACTCCCTTTTTTTCCGTTGTGGGCAAGGTGCATTCCACGCtttaa > 1:256580/1‑147 (MQ=255) tGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGt < 1:194581/148‑1 (MQ=255) | TGGCCGACGGTAGCCTGGTGGTGCCGTGGAACCATGCAATGCGCAGTGCCGGAGCGCA‑TTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGGCGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAA‑G‑GGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGT > NC_002947/6004820‑6005107 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |