Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,013,838	A→G	13.0%	intergenic (+43/+67)	xpt → / ← PP_5266	xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,013,838	0	A	G	13.0%	131.7 / 9.3	55	intergenic (+43/+67)	xpt/PP_5266	xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base A (18/29);  new base G (5/2);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01

TTCCAGGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCC  >  NC_002947/6013694‑6013977                                                                                                                                                 |                                                                                                                                             ttCCAGGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTgcagca                                                                                                                                           >  4:76972/1‑148 (MQ=255)   ccAGGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGcaca                                                                                                                                         <  7:137307/148‑1 (MQ=255)   ccAGGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGcaca                                                                                                                                         <  8:173606/148‑1 (MQ=255)    cAGGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAg                                                                                                                                        <  7:236295/148‑1 (MQ=255)     aGGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGt                                                                                                                                      >  1:161771/1‑149 (MQ=255)      gggCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACCCCCGCTCCCCCAGGTGGAGCACAgg                                                                                                                                       >  8:200221/1‑147 (MQ=255)            ccGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCACCCCCACGGGCACCACACGACTCGAAc                                                                                                                               >  2:80092/1‑149 (MQ=255)                   gAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCtg                                                                                                                           <  7:191816/146‑1 (MQ=255)                   gAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCtg                                                                                                                           >  8:191816/1‑146 (MQ=255)                   gAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑c                                                                                                                       <  3:76972/149‑1 (MQ=255)                    aaCTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCCCCCCCCCAACCACAGGTGCCGCACAAGGCTGCAACCCGGTG‑cc                                                                                                                      >  2:81738/1‑149 (MQ=255)                    aaCTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑cc                                                                                                                      >  4:181446/1‑149 (MQ=255)                       tGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑cc                                                                                                                      >  4:73442/1‑146 (MQ=255)                       tGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑cc                                                                                                                      <  3:73442/146‑1 (MQ=255)                       tGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCCAACGCTGTG‑ccct                                                                                                                    >  5:67570/1‑146 (MQ=255)                            aGCCAGGGTTACCGCGTTGAATCGCTGTCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCtgt                                                                                                              <  7:201519/149‑1 (MQ=255)                            aGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCtgt                                                                                                              <  6:49165/149‑1 (MQ=255)                            aGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCtgt                                                                                                              <  4:51966/149‑1 (MQ=255)                              ccAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTgg                                                                                                            <  2:161771/149‑1 (MQ=255)                                   gTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCg                                                                                                       <  3:181446/149‑1 (MQ=255)                                        cgcgTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCCCCCCCAACCGGGCAGCCCAGGTCGAGCACGCGGTG‑GCGTGCCTGGGGGAGCGGGCgg                                                                                                  >  3:121963/1‑148 (MQ=255)                                           gTTGAAGCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGGGGTCAGCTTCATCGAATAAACGCGACACCTGATTCGCGGGCACGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTG‑CTGCACCTGTGGGAGCGGGCGTGcc                                                                                               <  7:59586/149‑1 (MQ=18)                                                 tCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCg                                                                                                                     <  3:46733/121‑1 (MQ=255)                                                 tCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCg                                                                                                                     >  4:46733/1‑121 (MQ=255)                                                         ccGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTGTCGAACGCTGTG‑GCGGACCTGTGGGTGCGGGCGTGCCCCCGAATTGCCGc                                                                                  >  4:80995/1‑148 (MQ=38)                                                               gAAGTCGCTGGAAGGTGGTGGGGTCAGCTTCATCGAATAACCGCGACACCTGCTGCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCtt                                                                           <  7:200221/149‑1 (MQ=255)                                                                acgTCGCGGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGCCACCTGCTTCGCGGGCACGCCCGCTCCCACCGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCttt                                                                          <  2:181852/147‑1 (MQ=255)                                                                   tCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCTTACCTGTGGGAGCGGGTGTGCCCGCGAATGGCCGCAACCCtttt                                                                         <  6:285279/147‑1 (MQ=255)                                                                   tCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGTGTGCCCGCGAATGGCCGCAACCCtttt                                                                         >  5:285279/1‑147 (MQ=255)                                                                    cGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTTTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATc                                                                      <  1:80092/149‑1 (MQ=255)                                                                     gCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATc                                                                      <  6:67570/148‑1 (MQ=255)                                                                     gCTGGAAGGTGGTGTGGGCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCt                                                                     <  1:81738/149‑1 (MQ=255)                                                                                     gtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCCCAGGTGCAGCACAGGTCTCGAACGCGGTG‑CCGGACCGGTGGGAGCGGGCGTGCCCGCGAATGGCCGCCACCCTTTTTTATGGTTgcc                                                              >  2:87066/1‑140 (MQ=37)                                                                                     gtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTgcc                                                              <  1:87066/141‑1 (MQ=255)                                                                                       cAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGc                                                   <  3:80995/149‑1 (MQ=255)                                                                                               tCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCcaacaac                                            >  3:82079/1‑148 (MQ=255)                                                                                                               ccTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGttc                           <  8:290339/149‑1 (MQ=255)                                                                                                                cTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑GCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCACGCCTTTTATCTGGTTGCCGCCAGCCCCGGCAACAACAAGCGCTGGTAAAGttct                          >  7:274286/1‑149 (MQ=37)                                                                                                                cTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGc                                                                                     >  1:43102/1‑90 (MQ=255)                                                                                                                cTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGc                                                                                     <  2:43102/90‑1 (MQ=255)                                                                                                                   cTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAg                              <  7:220493/142‑1 (MQ=255)                                                                                                                   cTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGttctt                         <  4:82079/147‑1 (MQ=255)                                                                                                                   cTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGttctt                         <  3:107057/147‑1 (MQ=255)                                                                                                                   cTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCCCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACCAGCGCTGGTAAAg                              >  8:220493/1‑142 (MQ=255)                                                                                                                    ttCGCGGGCACGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTG‑CTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTg                                    <  5:94856/135‑1 (MQ=17)                                                                                                                    ttCGCGGGCACGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTG‑CTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTg                                    >  6:94856/1‑135 (MQ=17)                                                                                                                       gctggCACGCCGGCTCCCACAGGTGCAGCACAGGTATCGAACGCTGTG‑CCTTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTaa                   <  3:201974/146‑1 (MQ=38)                                                                                                                             actcccGCTCCCACAGGTACGTCACATCGTTCGAGACCTGTT‑ATGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTCTCTGGTTGCCGCCCGCCCAGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCt             >  6:278580/4‑149 (MQ=17)                                                                                                                             aCGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTG‑CTGCCCCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCt             >  6:283538/1‑149 (MQ=18)                                                                                                                                 ccGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCgg         <  3:53805/149‑1 (MQ=255)                                                                                                                                     tCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc           >  3:69343/1‑143 (MQ=255)                                                                                                                                     tCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc           <  4:69343/143‑1 (MQ=255)                                                                                                                                       ccACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc   <  2:30487/149‑1 (MQ=255)                                                                                                                                       ccACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc   >  7:100308/1‑149 (MQ=255)                                                                                                                                       ccACAGGTACGGCACAGCGTTCGAGACCTGTG‑CTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc   >  7:180452/1‑149 (MQ=21)                                                                                                                                         acaGGTACGGCACAGCGTTCGAGCCCTGTG‑CTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcccc  >  2:282903/1‑148 (MQ=25)                                                                                                                                                 |                                                                                                                                             TTCCAGGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCC  >  NC_002947/6013694‑6013977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑