Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,013,845 G→C 13.0% intergenic (+50/+60) xpt → / ← PP_5266 xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,013,8450GC13.0% 141.3 / 11.3 53intergenic (+50/+60)xpt/PP_5266xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base G (19/27);  new base C (5/2);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.14e-01

AGGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCC  >  NC_002947/6013698‑6013977
                                                                                                                                                   |                                                                                                                                     
aGGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGt                                                                                                                                      >  1:161771/1‑149 (MQ=255)
 gggCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACCCCCGCTCCCCCAGGTGGAGCACAgg                                                                                                                                       >  8:200221/1‑147 (MQ=255)
       ccGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCACCCCCACGGGCACCACACGACTCGAAc                                                                                                                               >  2:80092/1‑149 (MQ=255)
              gAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCtg                                                                                                                           <  7:191816/146‑1 (MQ=255)
              gAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCtg                                                                                                                           >  8:191816/1‑146 (MQ=255)
              gAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑c                                                                                                                       <  3:76972/149‑1 (MQ=255)
               aaCTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCCCCCCCCCAACCACAGGTGCCGCACAAGGCTGCAACCCGGTG‑cc                                                                                                                      >  2:81738/1‑149 (MQ=255)
               aaCTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑cc                                                                                                                      >  4:181446/1‑149 (MQ=255)
                  tGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑cc                                                                                                                      >  4:73442/1‑146 (MQ=255)
                  tGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑cc                                                                                                                      <  3:73442/146‑1 (MQ=255)
                  tGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCCAACGCTGTG‑ccct                                                                                                                    >  5:67570/1‑146 (MQ=255)
                       aGCCAGGGTTACCGCGTTGAATCGCTGTCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCtgt                                                                                                              <  7:201519/149‑1 (MQ=255)
                       aGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCtgt                                                                                                              <  4:51966/149‑1 (MQ=255)
                       aGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCtgt                                                                                                              <  6:49165/149‑1 (MQ=255)
                         ccAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTgg                                                                                                            <  2:161771/149‑1 (MQ=255)
                              gTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCg                                                                                                       <  3:181446/149‑1 (MQ=255)
                                   cgcgTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCCCCCCCAACCGGGCAGCCCAGGTCGAGCACGCGGTG‑GCGTGCCTGGGGGAGCGGGCgg                                                                                                  >  3:121963/1‑148 (MQ=255)
                                      gTTGAAGCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGGGGTCAGCTTCATCGAATAAACGCGACACCTGATTCGCGGGCACGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTG‑CTGCACCTGTGGGAGCGGGCGTGcc                                                                                               <  7:59586/149‑1 (MQ=18)
                                            tCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCg                                                                                                                     >  4:46733/1‑121 (MQ=255)
                                            tCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCg                                                                                                                     <  3:46733/121‑1 (MQ=255)
                                                    ccGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTGTCGAACGCTGTG‑GCGGACCTGTGGGTGCGGGCGTGCCCCCGAATTGCCGc                                                                                  >  4:80995/1‑148 (MQ=38)
                                                          gAAGTCGCTGGAAGGTGGTGGGGTCAGCTTCATCGAATAACCGCGACACCTGCTGCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCtt                                                                           <  7:200221/149‑1 (MQ=255)
                                                           acgTCGCGGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGCCACCTGCTTCGCGGGCACGCCCGCTCCCACCGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCttt                                                                          <  2:181852/147‑1 (MQ=255)
                                                              tCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCTTACCTGTGGGAGCGGGTGTGCCCGCGAATGGCCGCAACCCtttt                                                                         <  6:285279/147‑1 (MQ=255)
                                                              tCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGTGTGCCCGCGAATGGCCGCAACCCtttt                                                                         >  5:285279/1‑147 (MQ=255)
                                                               cGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTTTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATc                                                                      <  1:80092/149‑1 (MQ=255)
                                                                gCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATc                                                                      <  6:67570/148‑1 (MQ=255)
                                                                gCTGGAAGGTGGTGTGGGCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCt                                                                     <  1:81738/149‑1 (MQ=255)
                                                                                gtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCCCAGGTGCAGCACAGGTCTCGAACGCGGTG‑CCGGACCGGTGGGAGCGGGCGTGCCCGCGAATGGCCGCCACCCTTTTTTATGGTTgcc                                                              >  2:87066/1‑140 (MQ=37)
                                                                                gtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTgcc                                                              <  1:87066/141‑1 (MQ=255)
                                                                                  cAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGc                                                   <  3:80995/149‑1 (MQ=255)
                                                                                          tCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCcaacaac                                            >  3:82079/1‑148 (MQ=255)
                                                                                                          ccTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGttc                           <  8:290339/149‑1 (MQ=255)
                                                                                                           cTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑GCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCACGCCTTTTATCTGGTTGCCGCCAGCCCCGGCAACAACAAGCGCTGGTAAAGttct                          >  7:274286/1‑149 (MQ=37)
                                                                                                           cTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGc                                                                                     >  1:43102/1‑90 (MQ=255)
                                                                                                           cTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGc                                                                                     <  2:43102/90‑1 (MQ=255)
                                                                                                              cTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAg                              <  7:220493/142‑1 (MQ=255)
                                                                                                              cTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGttctt                         <  4:82079/147‑1 (MQ=255)
                                                                                                              cTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGttctt                         <  3:107057/147‑1 (MQ=255)
                                                                                                              cTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCCCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACCAGCGCTGGTAAAg                              >  8:220493/1‑142 (MQ=255)
                                                                                                               ttCGCGGGCACGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTG‑CTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTg                                    <  5:94856/135‑1 (MQ=17)
                                                                                                               ttCGCGGGCACGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTG‑CTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTg                                    >  6:94856/1‑135 (MQ=17)
                                                                                                                  gctggCACGCCGGCTCCCACAGGTGCAGCACAGGTATCGAACGCTGTG‑CCTTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTaa                   <  3:201974/146‑1 (MQ=38)
                                                                                                                        actcccGCTCCCACAGGTACGTCACATCGTTCGAGACCTGTT‑ATGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTCTCTGGTTGCCGCCCGCCCAGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCt             >  6:278580/4‑149 (MQ=17)
                                                                                                                        aCGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTG‑CTGCCCCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCt             >  6:283538/1‑149 (MQ=18)
                                                                                                                            ccGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCgg         <  3:53805/149‑1 (MQ=255)
                                                                                                                                tCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc           <  4:69343/143‑1 (MQ=255)
                                                                                                                                tCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc           >  3:69343/1‑143 (MQ=255)
                                                                                                                                  ccACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc   >  7:100308/1‑149 (MQ=255)
                                                                                                                                  ccACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc   <  2:30487/149‑1 (MQ=255)
                                                                                                                                  ccACAGGTACGGCACAGCGTTCGAGACCTGTG‑CTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc   >  7:180452/1‑149 (MQ=21)
                                                                                                                                    acaGGTACGGCACAGCGTTCGAGCCCTGTG‑CTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcccc  >  2:282903/1‑148 (MQ=25)
                                                                                                                                                caGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTgg                                   >  3:233179/1‑103 (MQ=255)
                                                                                                                                                caGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTgg                                   <  4:233179/103‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                     
AGGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTG‑CCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCC  >  NC_002947/6013698‑6013977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑