Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 603,663 T→A 10.5% intergenic (+59/‑33) ribAB‑I → / → ribH bifunctional 3,4‑dihydroxy‑2‑butanone 4‑phosphate synthase/GTP cyclohydrolase II/6,7‑dimethyl‑8‑ribityllumazine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947603,6630TA10.5% 125.0 / 4.0 47intergenic (+59/‑33)ribAB‑I/ribHbifunctional 3,4‑dihydroxy‑2‑butanone 4‑phosphate synthase/GTP cyclohydrolase II/6,7‑dimethyl‑8‑ribityllumazine synthase
Reads supporting (aligned to +/- strand):  ref base T (19/23);  new base A (3/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCTGATGAGTTCGCCGATGAAGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTTGGTGGTTGGCCGCTTCAACAGCTTCGTTGTCGAAAGCCTGGTAAGCGGTGCCGTTGATGC  >  NC_002947/603525‑603811
                                                                                                                                          |                                                                                                                                                    
gcCTGATGAGTTCGCCGATGAAGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGc                                                                                                                                            >  7:276618/1‑149 (MQ=255)
  cTGATGAGTTCGCCGATGAAGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGctc                                                                                                                                          <  4:177699/149‑1 (MQ=255)
  cTGATGAGTTCGCCGATGAAGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCATACAAGCGGCCTCgaca                                                                                                                                          <  5:66019/149‑4 (MQ=255)
     atgaGTTCGCCGATGAAGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGctct                                                                                                                                         <  1:170817/147‑1 (MQ=255)
     atgaGTTCGCCGATGAAGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCtt                                                                                                                                        <  7:123118/148‑1 (MQ=255)
         gTTCGCCGATGAAGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGa                                                                                                                                   <  8:276618/149‑1 (MQ=255)
               cGATGAAGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCATACAAGCGGCCTc                                                                                                                                              <  7:277157/132‑1 (MQ=255)
               cGATGAAGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCATACAAGCGGCCTc                                                                                                                                              >  8:277157/1‑132 (MQ=255)
                gATGAAGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGaa                                                                                                                             >  2:56256/1‑148 (MQ=255)
                   gAAGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAata                                                                                                                           >  6:262020/1‑147 (MQ=255)
                    aaGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATc                                                                                                                         <  4:302531/148‑1 (MQ=255)
                          aaCGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGATGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGa                                                                                                                  <  3:293133/149‑1 (MQ=255)
                                atatCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTg                                                                                                             >  2:27798/1‑148 (MQ=255)
                                   tCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGa                                                                                                         >  1:36459/1‑149 (MQ=255)
                                   tCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGa                                                                                                         <  1:27798/149‑1 (MQ=255)
                                      ggATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCa                                                                                                      <  3:162490/149‑1 (MQ=255)
                                        aTTCGATCTTGAAGTTTTATAATACGTGCCCTCAGAGTGACTTGAGGCTGAATTTACTCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAATATGAGAATATCGGAATGACCCTGAATACCAt                                                                                                     <  5:153995/148‑1 (MQ=255)
                                             aTCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGAAACCTCGCTCTTGAAGATGAGAATTTCGGAATGACCCTGAAGACCAACGAAgg                                                                                               >  1:259759/1‑149 (MQ=255)
                                                tGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGAcc                                                                                                       <  7:33488/138‑1 (MQ=255)
                                                tGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCCCGTGTTTGCGGCCACGCGGTTGAAGATGAGAATATAGGAATTACCCAGAAGACCaaaaaagg                                                                                               >  8:33488/1‑139 (MQ=255)
                                                  gAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAg                                                                                                <  5:262020/143‑1 (MQ=255)
                                                    aGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCa                                                                                                      <  8:282099/135‑1 (MQ=255)
                                                    aGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCa                                                                                                      >  7:282099/1‑135 (MQ=255)
                                                              tACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGccccc                                                                               >  5:227305/1‑148 (MQ=255)
                                                                 gTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGctct                                                                                                                                         <  8:92038/87‑1 (MQ=255)
                                                                 gTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGctct                                                                                                                                         >  7:92038/1‑87 (MQ=255)
                                                                                 ttGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAg                                                                                                          <  1:157997/102‑1 (MQ=255)
                                                                                  aatggcggcATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTtg                                                             <  1:271680/143‑1 (MQ=255)
                                                                                    aGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCCAGGCCCCTTGTATGCAGCCAAGCTCTACAAAATGATAATATAGGAATGAAACTAAAGACCAACCAAGGTACCTTCATCGCCCCCAAACGTCCCAATGCATTggtggt                                                        >  6:321463/1‑149 (MQ=255)
                                                                                     ggcggcATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTTGGTGGtt                                                       <  2:36459/149‑1 (MQ=255)
                                                                                          cATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGGCCCGTGTATTGGGGCGCGCGCATTAAAATGAGAATAAAGGAATGGCCCAGGAGACCCGAGAAAGTAAAATGATAGCCCCCAAACGTTGCGAAGCTTTTGTGGTTGGACg                                                  >  6:272656/1‑149 (MQ=255)
                                                                                           aTTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGGCTCGCTCTTGCAGATGAGAATATAGGGATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTAGGTATGATTTGGTGGTTGGCCGc                                                 >  5:145776/1‑149 (MQ=255)
                                                                                           aTTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCGTGTATGCGGCCTAGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTTGGTGGTTGGCCGc                                                 >  6:138593/1‑149 (MQ=255)
                                                                                                 gCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTAGCTCTTGAAGATGAGAATATAGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTTGGTGGTTGGCCGCTTCaa                                            >  5:72697/1‑148 (MQ=255)
                                                                                                   ccTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGt                                                                                              <  1:47524/96‑1 (MQ=255)
                                                                                                   ccTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGt                                                                                              >  2:47524/1‑96 (MQ=255)
                                                                                                            gAGTCCATACGCCTTTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCTATGGTACCTTCATCGCGCGCAAAGGTCGCTATGCTTTGGTGGTTGGCCGCTTCAACAGCTTCGTTg                                 <  5:321463/148‑1 (MQ=255)
                                                                                                                ccAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAATATGAGAATATCGGAATTACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTTGGTGGTTGGCCGCTTCAACAGCTTCGTTGTCGa                             <  2:259759/148‑1 (MQ=255)
                                                                                                                 cAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATAGCCCCCAAAGGTCGCTATGCTTTGGTGGTTGGCCGCTTaaa                                            >  7:207553/1‑132 (MQ=255)
                                                                                                                      cccTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTTGGTGGTTGGCCGCTTCAACAGCTTCGTTGTCGAAAGCCTg                      <  6:227305/149‑1 (MQ=255)
                                                                                                                         tGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTTGGTGGTTGGCCGCTTCAACa                                          <  2:74661/126‑1 (MQ=255)
                                                                                                                         tGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTTGGTGGTTGGCCGCTTCAACa                                          >  1:74661/1‑126 (MQ=255)
                                                                                                                          agcGAGGCCGCATACAAGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTTGGTGGTTGGCCGCTTCAACAGCTTCGTTGTCGAAAGCCTGGt                    >  3:155245/3‑147 (MQ=255)
                                                                                                                           gcGAGGCCGCATACAAGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTTGGTGGTTGGCCGCTTCAACAGCTTCGTTGTCGAAAGCCTGGTAAg                 >  6:67206/2‑149 (MQ=255)
                                                                                                                                   gCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTTGGTGGTTGGCCGCTTCAACAGCTTCGTTGTCGAAAGCCTGGTAAGCGGTGCCg         >  4:59839/1‑149 (MQ=255)
                                                                                                                                        tATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTTGGTGGTTGGCCGCTTCAACAGCTTCGTTGTCGAAAGCCTGGTAAGCGGTGCCGTTGa     <  1:56256/148‑1 (MQ=255)
                                                                                                                                         aTGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTTTGCTTTGGTGGTTGGCCGCTTCAACAGCTTCGTTGTCGAAAGCCTGGTAAGCGGTGCCGTTGAt    <  5:173406/148‑1 (MQ=255)
                                                                                                                                          tGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTTGGTGGTTGGCCGCTTCAACAGCTTCGTTGTCGAAAGCCTGGTAAGCGGTGCCGTTGATGc  >  2:191926/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                    
GCCTGATGAGTTCGCCGATGAAGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTTGGTGGTTGGCCGCTTCAACAGCTTCGTTGTCGAAAGCCTGGTAAGCGGTGCCGTTGATGC  >  NC_002947/603525‑603811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑