Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	615,007	C→T	6.3%	L80L (CTG→CTA)	ispA ←	farnesyl diphosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	615,007	0	C	T	6.3%	185.4 / 2.0	63	L80L (CTG→CTA)	ispA	farnesyl diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (31/28);  new base T (2/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.08e-01

TCAGGCAAGGGTCGAGCAGGGCACTGAAAGCCAGGCTCTGCAAACCGTCGCCAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAACGGGCGCACGCGTTTGCCGCCGTTCATCACGCTGTAGCGCATGGCGGCGTA  >  NC_002947/614859‑615153                                                                                                                                                     |                                                                                                                                                                tCAGGCAAGGGTCGAGCAGGGCACTGAAAGCCAGGCTCTGCAAACCGTCGCCAGCAAGGATGGCGCACGCTTCGTCAACCGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCAcc                                                                                                                                                                 >  7:14193/1‑149 (MQ=255)     gCAAGGGTCGAGCAGTGCACTGAAAGCCAGGCTCTGCAAACCGTCGCCAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGc                                                                                                                                                              <  6:291454/148‑1 (MQ=255)       aaGGGTCGAGCAGGGCACTGAAAGCCAGGCTCTGCAAACCGTCGCCAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAg                                                                                                                                                           >  3:98384/1‑149 (MQ=255)            tCGAGCAGGGCACTGAAAGCCAGGCTCTGCAAACCGTCGCCAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAg                                                                                                                                                       >  2:300200/1‑148 (MQ=255)              gAGCAGGGCACTGAAAGCCAGGCTCTGCAAACCGTCGCCAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCa                                                                                                                                                     <  3:265158/148‑1 (MQ=255)               aGCAGGGCACTGAAAGCCAGGCTCTGCAAACCGTCGCCAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATg                                                                                                                                                   >  7:152540/1‑149 (MQ=255)                gCAGGGCACTGAAAGCCAGGCTCTGCAAACCGTCGCCAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATgg                                                                                                                                                  <  4:143096/149‑1 (MQ=255)                         tGAAAGCCAGGCTCTGCAAACCGTCGCCAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACTAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑Tc                                                                                                                             >  5:103280/1‑148 (MQ=255)                         tGAAAGCCAGGCTCTGCAAACCGTCGCCAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACTAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑Tc                                                                                                                             >  5:98794/1‑148 (MQ=255)                           aaaGCCAGGCTCTGCAAACCGTCGCCAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACTAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑Tc                                                                                                                             <  6:98794/146‑1 (MQ=255)                           aaaGCCAGGCTCTGCAAACCGTCGCCAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACTAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑Tc                                                                                                                             <  6:103280/146‑1 (MQ=255)                           aaaGCCAGGCTCTGCAAACCGTCGCCAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGAc                                                                                                                          >  3:95949/1‑149 (MQ=255)                              gCCAGGCTCTGCAAACCGTCGCCAGCAAGGATGGCGCACGCTTCGTCACACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTaa                                                                                                                                                        <  6:286433/129‑1 (MQ=255)                              gCCAGGCTCTGCAAACCGTCGCCAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTaa                                                                                                                                                        <  6:176395/129‑1 (MQ=255)                              gCCAGGCTCTGCAAACCGTCGCCAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTaa                                                                                                                                                        >  5:176395/1‑129 (MQ=255)                              gCCAGGCTCTGCAAACCGTCGCCAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTaa                                                                                                                                                        >  5:286433/1‑129 (MQ=255)                                  ggCTCTGCAAACCGTCGCCAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAg                                                                                                                   <  4:42241/149‑1 (MQ=255)                                     tctGCAAACCGTCGCCAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCt                                                                                                                >  1:301181/1‑149 (MQ=255)                                         cAAACCGTCGCCAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCAc                                                                                                            >  7:273014/1‑149 (MQ=255)                                         cAAACCGTCGCCAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCAc                                                                                                            <  3:51675/149‑1 (MQ=255)                                           aaCCGTCGCCAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCAGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCCCCg                                                                                                          >  4:38045/1‑149 (MQ=255)                                            aCCGTCGCCAGCAAGGATGGCGAACGCTTAGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGt                                                                                                         >  2:101690/1‑149 (MQ=255)                                              cGTCGCCAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTg                                                                                                       <  4:98384/149‑1 (MQ=255)                                                    cAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGt                                                                                                 <  4:95949/149‑1 (MQ=255)                                                      gCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCTTGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTc                                                                                               >  1:183854/1‑149 (MQ=255)                                                             tGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCggg                                                                                        <  2:301181/149‑1 (MQ=255)                                                              ggCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCggg                                                                                        <  8:273014/148‑1 (MQ=255)                                                                         cGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCaa                                                                                <  5:233116/145‑1 (MQ=255)                                                                         cGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCaa                                                                                >  6:233116/1‑145 (MQ=255)                                                                          gTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCtt                                                                           <  1:300200/149‑1 (MQ=255)                                                                           tCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTc                                                                          >  6:253792/1‑149 (MQ=255)                                                                            cAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGTACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCg                                                                         >  3:296665/1‑149 (MQ=255)                                                                            cAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCg                                                                         >  5:297499/1‑149 (MQ=255)                                                                             aaaCGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCgggg                                                                                       <  8:266744/134‑1 (MQ=255)                                                                             aaaCGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCgggg                                                                                       >  7:266744/1‑134 (MQ=255)                                                                             aaaCGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCg                                                                         >  4:48032/1‑148 (MQ=255)                                                                             aaaCGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGc                                                                        <  3:48032/149‑1 (MQ=255)                                                                               aCGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCTTGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAg                                                                      <  2:183854/149‑1 (MQ=255)                                                                                  ccTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGcc                                                                   <  8:152540/149‑1 (MQ=255)                                                                                                 gCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAAc                                                    <  8:14193/149‑1 (MQ=255)                                                                                                     gCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGTACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGTCTTCGCAGGCCGCGTAGTCCAGCAACggg                                                 <  4:296665/148‑1 (MQ=255)                                                                                                             gCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCA‑CAGCGAGAGAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAACGGGCGCACGCGtt                                       >  1:229102/1‑149 (MQ=255)                                                                                                               aGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCAAGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAACGGGCGCACGCGttt                                      <  7:50420/149‑1 (MQ=255)                                                                                                                 gtcgtcgtcgtcCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAACGGGCGCACGCGTTTgc                                    >  5:159788/1‑149 (MQ=255)                                                                                                                 gtcgtcgtcgtcCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAACGGGCGCACGCGTTTgc                                    >  8:30636/1‑149 (MQ=255)                                                                                                                  tcgtcgtcgtcCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAg                                                                      <  5:239142/114‑1 (MQ=255)                                                                                                                  tcgtcgtcgtcCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAg                                                                      >  6:239142/1‑114 (MQ=255)                                                                                                                    gtcgtcgtcCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGCCTCAGCTTCCCCTTCTTCTGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAACGGgcgc                                              <  1:101690/149‑1 (MQ=255)                                                                                                                            ccATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAACGGGCGCACGCGTTTGCCGCCGTtcat                          >  6:8291/1‑148 (MQ=255)                                                                                                                             cATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAACGGGCGCACGCGTTTGCCGCCGTtcatca                        <  6:297499/149‑1 (MQ=255)                                                                                                                             cATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAACGGGCGCACGCGTTTGCCGCCGTtcatca                        >  5:281221/1‑149 (MQ=255)                                                                                                                               tGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCa                                                         >  3:29235/1‑114 (MQ=255)                                                                                                                               tGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCa                                                         <  4:29235/114‑1 (MQ=255)                                                                                                                                  ctgggAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAACGGGCGCACGCGTTTGCCGCCGTTCATCACGCTg                   >  8:242039/3‑149 (MQ=255)                                                                                                                                  ccGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAACGGGCGCACGCGTTTGCCGCCGTTCATCACGCTg                   >  6:78925/1‑149 (MQ=255)                                                                                                                                               aTGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAACGGGCGCACGCGTTTGCCGCCGTTCATCACGCTGTAGCGCATggcgg      >  7:46313/1‑149 (MQ=255)                                                                                                                                                tGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAACGGGCGCACGCGTTTGCCGCCGTtcatc                         >  7:284720/1‑129 (MQ=255)                                                                                                                                                tGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAACGGGCGCACGCGTTTGCCGCCGTtcatc                         <  8:284720/129‑1 (MQ=255)                                                                                                                                                tGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAACGGGCGCACGCGTTTGCCGCCGTTCATCACGCTGTAGCGCATggcggc     <  6:159788/149‑1 (MQ=255)                                                                                                                                                  cACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGACCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAACGGGCGCACGCGTTTGCCGCCGTTCATCACGCTGTAGCGCATggc        <  7:100917/144‑1 (MQ=255)                                                                                                                                                  cACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGACCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAACGGGCGCACGCGTTTGCCGCCGTTCATCACGCTGTAGCGCATggc        >  8:100917/1‑144 (MQ=255)                                                                                                                                                   aCCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAACGGGCGCACGCGTTTGCCGCCGTTCATCACGCTGTa                 <  6:25471/134‑1 (MQ=255)                                                                                                                                                   aCCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAACGGGCGCACGCGTTTGCCGCCGTTCATCACGCTGTa                 >  5:25471/1‑134 (MQ=255)                                                                                                                                                   aCCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAACGGGCGCACGCGTTTGCCGCCGTTCATCACGCTGTAGCGCATGGCGGCGt   >  1:84277/1‑148 (MQ=255)                                                                                                                                                     cAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAACGGGCGCACGCGTTTGCCGCCGTTCATCACGCTGTAGCGCATGGCGGCGTa  >  7:239753/1‑147 (MQ=255)                                                                                                                                                     |                                                                                                                                                                TCAGGCAAGGGTCGAGCAGGGCACTGAAAGCCAGGCTCTGCAAACCGTCGCCAGCAAGGATGGCGCACGCTTCGTCAAACGCCTTGTGGGTGGTCGGCTGGCCGCGACGCAGGTCGTCGTCGTCCATGGCCGGGAGGTCGTCATGCACCAGCGAGTAAGCATGGATCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑TCGACCGCGCAGGCTGCACCGTTGGCCTGTTCGGCCGGGGCGCCCAAGGCTTCGCAGGCCGCGTAGGCCAGCAACGGGCGCACGCGTTTGCCGCCGTTCATCACGCTGTAGCGCATGGCGGCGTA  >  NC_002947/614859‑615153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑