Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,075,775 G→A 10.0% H263Y (CAC→TAC)  PP_5328 ← phosphate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,075,7750GA10.0% 109.6 / 3.1 40H263Y (CAC→TAC) PP_5328phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (18/18);  new base A (2/2);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TGATCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGCTCGACCACCTGCAGATGAGCACCGGTAGAGCCGGC  >  NC_002947/6075628‑6075922
                                                                                                                                                   |                                                                                                                                                   
tgatCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGc                                                                                                                                                    <  5:78810/149‑1 (MQ=255)
                 ccAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATg                                                                                                                                     >  3:160199/1‑147 (MQ=255)
                                gTGGCAGTAATTTCGCTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGACCACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGt                                                                                                                    <  4:206236/149‑1 (MQ=255)
                                            tCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCAttt                                                                                                         <  8:79739/148‑1 (MQ=255)
                                            tCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTg                                                                                                        >  2:119817/1‑149 (MQ=255)
                                             cGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTgg                                                                                                                                            >  6:71431/1‑112 (MQ=255)
                                             cGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTgg                                                                                                                                            <  5:71431/112‑1 (MQ=255)
                                             cGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTg                                                                                                        <  1:51330/148‑1 (MQ=255)
                                              ggTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCg                                                                                                      <  2:105948/149‑1 (MQ=255)
                                                  tCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCa                                                                                                   <  8:95116/148‑1 (MQ=255)
                                                    ggCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTACTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGtt                                                                                                >  2:150061/1‑149 (MQ=255)
                                                    ggCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTACTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGtt                                                                                                >  2:154885/1‑149 (MQ=255)
                                                         tATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCCTATCGGTCATTTGCGGCAGTTAGATg                                                                                           >  8:237643/1‑149 (MQ=255)
                                                                   cTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGAt                                                                                                                                      >  2:205950/1‑96 (MQ=255)
                                                                   cTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGAt                                                                                                                                      <  1:205950/96‑1 (MQ=255)
                                                                         aCGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCggtg                                                                            <  7:237643/148‑1 (MQ=255)
                                                                                ttGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACctc                                                                    >  1:309822/1‑149 (MQ=255)
                                                                                  gAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGtt                                                                                                <  8:123322/119‑1 (MQ=255)
                                                                                  gAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGtt                                                                                                >  7:123322/1‑119 (MQ=255)
                                                                                         ttGTCGCCCAGGCTGAACACGTCCGCCCTGGCACGGCCGATGATCACGTACAGCCAGTGATGGCGTGGGTCGATGAAGATATTATTCACCGGCTAGGTCATTTGCGGCAGTTCGATGCGGTTCTTCTCCGTGGTGACCTCGTCGGtcct                                                           >  8:163839/1‑147 (MQ=255)
                                                                                           gTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCg                                                                <  2:243623/142‑1 (MQ=255)
                                                                                           gTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCg                                                                >  1:243623/1‑142 (MQ=255)
                                                                                                   ggCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTcc                                                 <  4:160199/149‑1 (MQ=255)
                                                                                                      tGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTg                                              >  2:111231/1‑149 (MQ=255)
                                                                                                         acacGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCgg                                           <  6:118298/149‑1 (MQ=255)
                                                                                                         acacGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTACTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCgg                                           <  1:154885/149‑1 (MQ=255)
                                                                                                         acacGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTACTGGCGTGGGGCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCgg                                           <  1:150061/149‑1 (MQ=255)
                                                                                                            cGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTc                                        <  2:309822/149‑1 (MQ=255)
                                                                                                             gTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGt                                         >  2:279640/1‑147 (MQ=255)
                                                                                                                ggCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTTGCGTGGGTCGATGAAGATATTCTTCACCGTCTGGGGCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGc                                     <  7:163839/148‑1 (MQ=255)
                                                                                                                ggCCGTGGCACGGCCATTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCAt                                                                                                           <  5:31085/78‑1 (MQ=255)
                                                                                                                ggCCGTGGCACGGCCATTGATCACGTACAGCCAGTGCTGGCGTGGGACGATGAAGATATTCTTCACCGTCTCGGTCAt                                                                                                           >  6:31085/1‑78 (MQ=255)
                                                                                                                         aCGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGCTCGACCACCt                           >  6:211416/1‑149 (MQ=255)
                                                                                                                               gTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGCTCGACCACCTGCAGAt                     <  1:111231/149‑1 (MQ=255)
                                                                                                                                ttGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGCTCGACCACCTGCAGAt                     >  5:80546/1‑148 (MQ=255)
                                                                                                                                   aTCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGCTCGACCACCTGCAGAGGAGc                 >  4:66596/1‑149 (MQ=255)
                                                                                                                                         tACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGCTCGACCACCTGCAGATGAGCACCgg            >  3:9196/1‑148 (MQ=255)
                                                                                                                                              ccAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGCTCGACCACCTGCAGATGAGCACCGGTAGAGc      >  7:33459/1‑149 (MQ=255)
                                                                                                                                              ccAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGCTCGACCACCTGCAGATGAGCACCGGTAGAGc      >  3:271173/1‑149 (MQ=255)
                                                                                                                                                 gTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGCTCGACCACCTGCAGATGAGCACCGGTAGAGCCgg   <  1:119817/149‑1 (MQ=255)
                                                                                                                                                   gCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGCTCGACCACCTGCAGATGAGCACCGGTAGAGCCGGc  >  1:72368/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGATCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGCTCGACCACCTGCAGATGAGCACCGGTAGAGCCGGC  >  NC_002947/6075628‑6075922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑