Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,138,932 A→G 8.5% Y46C (TAC→TGC)  cusB → CzcB family copper RND transporter membrane fusion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,138,9320AG 8.5% 130.9 / 2.1 47Y46C (TAC→TGC) cusBCzcB family copper RND transporter membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base A (22/21);  new base G (2/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAGGTGCGTGCATGAACAGCAGGTCTATCGGTTTGATCACCGCAATCGCCCTGGCTATTGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATGGACATGCAACTGGTAGCGAAATACGCCGAAGAAGGCACAGACCCCGCAGAGCCAGCGGTGCAAATCAGCCAAGGCGTTCAGCAGAACCTTG  >  NC_002947/6138784‑6139078
                                                                                                                                                    |                                                                                                                                                  
cGAGGTGCGTGCATGAAGAGCATGTCTATCGGTTTGATCACCGCATTCGCCGTGGCTATTGTCGTCGGGACAGTCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTa                                                                                                                                                    <  6:214739/149‑1 (MQ=255)
       cgtgcATGAACAGCAGGTCTATCGGTTTGATCACCGCAATCGCCCTGGCTATTGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGAcccc                                                                                                                                             <  3:113388/149‑1 (MQ=255)
             tGAACAGCAGGTCTATCGGTTTGATCACCGCAATCGCCCTGGCTATTGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTAc                                                                                                                                       <  2:36874/149‑1 (MQ=255)
             tGAACAGCAGGTCTATCGGTTTGATCACCGCAATCGCCCTGGCTATTGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTAc                                                                                                                                       >  4:86531/1‑149 (MQ=255)
              gAACAGCAGGTCTATCGGTTTGATCACCGCAATCGCCCTGGCTATTGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTAcc                                                                                                                                      >  5:186335/1‑149 (MQ=255)
                aCAGCAGGTCTATCGGTTTGATCACCGCAATCGCCCTGGCTATTGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTAccc                                                                                                                                     <  4:179638/148‑1 (MQ=255)
                   gcagGTCTATCGGTTTGATCACCGCAATCGCCCTGGCTATTGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCgcag                                                                                                                                 >  4:281758/1‑149 (MQ=255)
                        tCTATCGGTTTGATCACCGCAATCGCCCTGGCTATTGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCgcagcagca                                                                                                                            <  3:86531/149‑1 (MQ=255)
                         cTATCGGTTTGATCACCGCAATCGCCCTGGCTATTGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCAc                                                                                                                           >  3:19703/1‑149 (MQ=255)
                           aTCGGTTTGATCACCGCAATCGCCCTGGCTATTGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTGCGACCCCATGTACCCGCAGCAGCACtt                                                                                                                         >  5:95756/1‑149 (MQ=255)
                           aTCGGTTTGATCACCGCAATCGCCCTGGCTATTGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACtt                                                                                                                         >  2:61370/1‑149 (MQ=255)
                               gTTTGATCACCGCAATCGCCCTGGCTATTGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCa                                                                                                                     <  3:281758/149‑1 (MQ=255)
                                    aTCACCGCAATCGCCCTGGCTATTGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGAACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTAAGACCCCATGTACCAGCAGCAGAACTTCCCAGAGcc                                                                                                                >  3:115612/1‑149 (MQ=255)
                                     tCACCGCAATCGCCCTGGCTATTGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGccc                                                                                                               >  7:280940/1‑149 (MQ=255)
                                       aCCGCAATCGCCCTGGCTATTGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCg                                                                                                              <  1:129879/148‑1 (MQ=255)
                                         cGCAATCGCCCTGGCTATTGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTGCGACCCCATGTACCCGCAGCAGCACTTCCCa                                                                                                                     >  5:173191/1‑139 (MQ=255)
                                         cGCAATCGCCCTGGCTATTGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTGCGACCCCATGTACCCGCAGCAGCACTTCCCa                                                                                                                     <  6:173191/139‑1 (MQ=255)
                                                    tGGCTATTGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAgcgc                                                                                                                 >  3:300712/1‑132 (MQ=255)
                                                    tGGCTATTGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAgcgc                                                                                                                 <  4:300712/132‑1 (MQ=255)
                                                      gCTATTGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTc                                                                                                <  6:186335/147‑1 (MQ=255)
                                                        tATTGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATg                                                                                             >  3:95618/1‑148 (MQ=255)
                                                          ttGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATg                                                                                             <  4:95618/146‑1 (MQ=255)
                                                             gCGTCGGGACAGGCTACTGGTTGGTACATCCTGCGCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCGAGCGCCCGGCAAGTCGCCATTCATGGACAt                                                                                        <  4:19703/148‑1 (MQ=255)
                                                             gCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTc                                                                                                >  7:43361/1‑140 (MQ=255)
                                                             gCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTc                                                                                                <  8:43361/140‑1 (MQ=255)
                                                                     aCAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCACCCGGCAAGTCGCCATTCATGGACATGCAACTgg                                                                                >  2:78349/1‑148 (MQ=255)
                                                                       aGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATGGACATGCAACTGGTAg                                                                             <  1:285513/149‑1 (MQ=255)
                                                                              tGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATGGACAt                                                                                        >  6:83474/1‑131 (MQ=255)
                                                                              tGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATGGACAt                                                                                        <  5:83474/131‑1 (MQ=255)
                                                                                gTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATGGACATGCAACTGGTAGCGAAATACg                                                                    >  6:138064/1‑149 (MQ=255)
                                                                                         tCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATGGACATGCAACTGGTAGCGAAATACGCCgaagaat                                                           >  5:264105/1‑148 (MQ=255)
                                                                                         tCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATGGACATGCAACTGGTAGCGAAATACGCCGaagaag                                                           >  4:155202/1‑149 (MQ=255)
                                                                                          ccGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATGGACATGCAACTGGTAGCGAAATACGCCGAAGAAgg                                                          >  1:84665/1‑149 (MQ=255)
                                                                                           cGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATGGACATGCAACTGGTAGCGAAATACGCCGAAGAAGGc                                                         <  5:138064/149‑1 (MQ=255)
                                                                                                  aCCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCACGCCAATTCGCCATTAATGGACATCCAACTGCTAGCGAAATACGCCGAAGAAGGCACAGaac                                                  >  3:181066/1‑147 (MQ=255)
                                                                                                    cTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTGCGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATGGACATGCAACTGGTAGCGAAATACGCCGAAGAAGGCACAGAcccc                                                <  6:95756/149‑1 (MQ=255)
                                                                                                       cacaAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATGGACATGCAACTGGTAGCGAAATACGCCGAAGAAGGCACAGACCCCGCa                                             <  1:61370/149‑1 (MQ=255)
                                                                                                         caAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATGGACATGCAACTGGTAGCGAAATACGCCGAAGAAGGCACAGACCCCGCaga                                           <  8:280940/149‑1 (MQ=255)
                                                                                                           aGCAACCGCAATAGCAACAGATCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATGGACATGCAACTTGTAGCGAAATACGCCGAATAAGGCACAGACCCCGCAGAGc                                         <  4:115612/149‑1 (MQ=255)
                                                                                                                ccGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATGGACATGCAACTGGTAGCGAAATACGCCGAAGAAGGCACAGACCCCGCAGAGCCAGCg                                    <  3:155202/149‑1 (MQ=255)
                                                                                                                  gCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCGCGGCAAGTCGCCTTTCATTGACATTCAACTGGTAGATAAATTCGCCGAAGAAGGGACAGACCCCGCAGAGCCacccga                                  <  6:281163/149‑6 (MQ=255)
                                                                                                                     aTAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATGGACATGCAACTGGTAGCGAAATACGCCGAAGAAGGCACAGACCCCGCAGAGCCAGCGGTGCa                               <  5:129285/149‑1 (MQ=255)
                                                                                                                       aGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATGGACATGCAACTGGTAGCGAAATACGCCGAAGAAGGCACAGACCCCGCAGAGCCAGCGGTGCaaa                             >  4:93198/1‑149 (MQ=255)
                                                                                                                       aGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATGGACATGCAACTGGTAGCGAAATACGCCGAAGAAGGCACAGACCCCGCAGAGCCAGCGGTGCaa                              >  3:223316/1‑148 (MQ=255)
                                                                                                                           aCAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATGGACATGCAACTGGTAGCGAAATACGCCGAAGAAGGCACAGACCCCGCAGAGCCAGCGGTGCAAATCAg                         >  7:144503/1‑149 (MQ=255)
                                                                                                                                 cAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATGGACATGCAACTGGTAGCGAAATACGCCGAAGAAGGCACAGACCCCGCAGAGCCAGCGGTGCAAATCAGCCAAgg                   >  5:235366/1‑149 (MQ=255)
                                                                                                                                             tATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATGGACATGCAACTGGTAGCGAAATACGCCGAAGAAGGCACAGACCCCGCAGAGCCAGCGGTGCAAATCAGCCAAGGCGTTCAGCAGaa       <  2:84665/149‑1 (MQ=255)
                                                                                                                                                  gTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATGGACATGCAACTGGTAGCGAAATACGCCGAAGAAGGCACAGACCCCGCAGAGCCAGCGGTGCAAATCAGCCAAGGCGTTCAGCAGAACCTTg  >  2:229042/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGAGGTGCGTGCATGAACAGCAGGTCTATCGGTTTGATCACCGCAATCGCCCTGGCTATTGGCGTCGGGACAGGCTACTGGTTGGGACATCCGGCCCAACCTGCACAAGCACCCGCAATAGCAACAGAGCAGAAGCCGTTGTATTGGTACGACCCCATGTACCCGCAGCAGCACTTCCCAGCGCCCGGCAAGTCGCCATTCATGGACATGCAACTGGTAGCGAAATACGCCGAAGAAGGCACAGACCCCGCAGAGCCAGCGGTGCAAATCAGCCAAGGCGTTCAGCAGAACCTTG  >  NC_002947/6138784‑6139078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑