| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,155,242 | C→G | 19.7% | intergenic (‑52/+154) | PP_5398 ← / ← PP_5399 | transposase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,155,242 | 0 | C | G | 19.7% | 78.7 / 11.1 | 35 | intergenic (‑52/+154) | PP_5398/PP_5399 | transposase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (13/15); new base G (5/2); total (18/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCAATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCAGAGTTGTACTTTCTGGACGCAGGGAAATCAGGTGCGGAAGTACTGCAGAATTTCCTCAAAGTTTCCAA > NC_002947/6155148‑6155385 | ccTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCAATAAGGGTGATCGCGTCAGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGGTCGGAAGGGGAGTTCCCCGGACGCTTACTCGCTg < 3:184923/149‑1 (MQ=255) cTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCAATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGTAGTTCGGAAGGTGAGTTCCACGGACGCTTACTCGCTg > 7:288532/1‑148 (MQ=21) cTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCAATAAGGGTGATCGCGTCAGCTTAAAAATACGCGTACACCATCGCCCTTAATGATGGAGTTCGGAAGGTGAGTTCCCAGGACGCTTACTCGCTg > 7:292555/1‑148 (MQ=255) tGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCAATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTa > 8:250958/1‑149 (MQ=35) gAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCAATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTc > 2:154212/1‑148 (MQ=255) aaCGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCAATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTc > 6:20176/1‑147 (MQ=255) aCGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCAATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTc > 8:25173/1‑146 (MQ=255) tttCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCAATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACgg < 5:319722/149‑1 (MQ=255) tttCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCAATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACgg < 3:142354/149‑1 (MQ=255) cGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCAATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGa < 4:34661/148‑1 (MQ=255) tAAGAGCTTCGTTGGCGCATGGAAATCCTGGCAATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCaga < 7:250958/149‑1 (MQ=255) aaGAGCTTCGTTGGCGCATGGAAATCCTGGCAATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCagag < 4:61446/149‑1 (MQ=255) gagCTTCGTTGGCGCATGGAAATCCTGGCAATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCAGAGtt < 6:322615/149‑1 (MQ=255) gagCTTCGTTGGCGCATGGAAATCCTGGCAATAAGGGTGATCGCGTCCGCTTAAAAATACGCAGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCAGAGtt > 1:93971/1‑149 (MQ=255) gagCTTCGTTGGCGCATGGAAATCCTGGCAATAAGGGTGATCGCGGCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCAGAGtt < 6:324792/149‑1 (MQ=255) agCTTCGTTGGCGCATGGAAATCCTGGCAATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCAGAGTTg > 4:64758/1‑149 (MQ=255) tGGAAATCCTGGCAATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCAGAGTTGTACTTTCTGGACGCa < 7:25173/149‑1 (MQ=255) aaaTCCTGGCATTAAGGGTGATCGCTTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCATAGTTGTACTTTCTGGACGCAgg < 4:1351/148‑1 (MQ=255) aaTCCTGGCAATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCAGAGTTGTACTTTCTGGACGCAggg > 3:270123/1‑148 (MQ=255) ccTGGCAATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACGATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACGCGGTGTATGCGTTGGAGACGGGAGCAGAGTTGTACTTTCTGGACGCAGGGAAAt > 5:250067/1‑149 (MQ=255) gCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCaga > 6:203274/1‑119 (MQ=17) gCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCaga < 5:203274/119‑1 (MQ=17) gCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCAGAGTTGTACTTTCTGGACGCAGGGAAATCAgg > 8:110724/1‑149 (MQ=25) gCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCAGAGTTGTACTTTCTGGACGCAGGGAAATCAgg > 8:115021/1‑149 (MQ=25) attAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCAGAGTTGTACTTTCTGGACGCAGGGAAATCAGGTg > 4:201372/3‑149 (MQ=25) gggTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCagag < 6:293957/113‑1 (MQ=255) gggTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCagag > 5:293957/1‑113 (MQ=255) aTCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCAGAGTTGTACTTTCTGGACGCAg < 2:314517/127‑1 (MQ=255) aTCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCAGAGTTGTACTTTCTGGACGCAg > 1:314517/1‑127 (MQ=255) gcgTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCAGAGTTGTACTTTCTGGACGCAGGGAAATCAGGTGCGGAAGTACTGCa < 5:20176/149‑1 (MQ=255) cgTCGGCTTATAAATACGCGGACACGATCGCGCTTAATGCTGGAGTTCGGAAGGTGAGTTCCGCGGACGGTTTCTCGCTGTTTGCGTTCGAGACGGGAGCAGAGTTGTACTTTCTGGTCGCAGGGAAATCAGGTGCGGAAGTACTGCAg < 1:188534/149‑1 (MQ=25) ttAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCAGAGTTGTACTTTCTGGACGCAgg > 6:41012/1‑117 (MQ=255) ttAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCAGAGTTGTACTTTCTGGACGCAgg < 5:41012/117‑1 (MQ=255) aaaTACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCAGAGTTGTACTTTCTGGACGCAGGGAAATCAGGTGCGGAAGTACTGCAGAATTTCCTCa > 6:42939/1‑148 (MQ=255) gACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCAGAGTTGTACTTTCTGGACGCAGGGAAATCAGGTGCGGAAGTACTGCAGAATTTCCTCAAAGTTTCCaa < 5:42939/149‑1 (MQ=255) | CCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCAATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCCCCGGACGCTTACTCGCTGTATGCGTTCGAGACGGGAGCAGAGTTGTACTTTCTGGACGCAGGGAAATCAGGTGCGGAAGTACTGCAGAATTTCCTCAAAGTTTCCAA > NC_002947/6155148‑6155385 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |