Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,178,242 C→G 9.7% R279P (CGC→CCC)  atpA ← ATP synthase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,178,2420CG 9.7% 122.0 / 2.8 41R279P (CGC→CCC) atpAATP synthase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (23/14);  new base G (2/2);  total (25/16)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGAACCGGTTTTGCCAGTGACAGCACCGTTGGTGAACTTCTCGACGTACTCTTCCGAAACGCGCGATGCACGCTCCAGCAGACGGGAGTGGAGATAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGCCCATGGTGCAACCGGCGTATGGCGCCAGGAATTGCAGTGCGGCGGATTCC  >  NC_002947/6178105‑6178388
                                                                                                                                         |                                                                                                                                                  
gggAACCGGTTTTGCCAGTGACAGCACCGTTGGTGAACTTCTCGACGTACTCTTCCGAAACGCGCGATGCACGCTCCAGCAGACGGGAGTGGAGATAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAgg                                                                                                                                         <  7:90014/149‑1 (MQ=255)
            tGCCAGTGACAGCACCGTTGGTGAACTTCTCGACGTACTCTTCCGAAACGCGCGATGCACGCTCCAGCAGACGGGAGTGGAGATAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACg                                                                                                                              <  8:102772/148‑1 (MQ=255)
             gCCAGTGACAGCACCGTTGGTGAACTTCTCGACGTACTCTTCCGAAACGCGCGATGCACGCTCCAGCAGACGGGAGTGGAGATAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACgg                                                                                                                             <  6:204799/148‑1 (MQ=255)
                                gTGAACTTCTCGACGTACTCTTCCGAAACGCGCGATGCACGCTCCAGCAGACGGGAGTGGAGATAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGa                                                                                                         >  1:190453/1‑149 (MQ=255)
                                 tGAACTTCTCGACGTACTCTTCCGAAACGCGCGATGCACGCTCCAGCAGACGGGAGTGGAGATAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGa                                                                                                         >  5:121821/1‑148 (MQ=255)
                                 tGAACTTCTCGACGTACTCTTCCGAAACGCGCGATGCACGCTCCAGCAGACGGGAGTGGAGATAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGa                                                                                                         >  6:317769/1‑148 (MQ=255)
                                  gAACTTCTCGACGTACTCTTCCGAAACGCGCGATGCACGCTCCAGCAGACGGGAGTGGAGATAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGAc                                                                                                        <  6:121821/148‑1 (MQ=255)
                                     cTTCTCGACGTACTCTTCCGAAACGCGCGATGCACGCTCCAGCAGACGGGAGTGGAGATAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGt                                                                                                    <  5:317769/149‑1 (MQ=255)
                                           gACGTACTCTTCCGAAACGCGCGATGCACGCTCCAGCAGACGGGAGTGGAGATAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCg                                                                                               >  4:274695/1‑148 (MQ=255)
                                                 ctctTCCGAAACGCGCGATGCACGCTCCAGCAGACGGGAGTGGAGATAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGa                                                                                        >  3:302692/1‑149 (MQ=255)
                                                    ttCCGAAACGCGCGATGCACGCTCCAGCAGACGGGAGTGGAGATAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGAt                                                                                       >  8:282843/1‑147 (MQ=255)
                                                    ttCCGAAACGCGCGATGCACGCTCCAGCAGACGGGAGTGGAGATAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGAt                                                                                       >  7:130528/1‑147 (MQ=255)
                                                                  aTGTACGCTCCAGCAGACGGGAGTGGAGATAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACcg                                                                       >  4:124229/1‑149 (MQ=255)
                                                                        gCTCCAGCAGACGGGAGTGGAGATAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTcg                                                                 >  2:56838/1‑149 (MQ=255)
                                                                             agcagACGGGAGTGGAGATAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGgaa                                                            <  2:190453/149‑1 (MQ=255)
                                                                             agcagACGGGAGTGGAGATAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGgaa                                                            <  1:56838/149‑1 (MQ=255)
                                                                              gcagACGGGAGTGGAGATAGAACACGTATCCTGGGTAAGCTTCGCGTCCTGGAGGACGGCGCAGCAGCAGGGAGATCTCACGGTAGGCAACCGCCTGCATGTACAGGTCATCGTAAACGAACAGTGAGTCTTCa                                                                          >  3:86602/1‑134 (MQ=255)
                                                                               cagACGGGAGTGGAGATAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGgaag                                                           >  1:13672/1‑148 (MQ=255)
                                                                                     ggAGTGGAGATAGAACACGTCGCCTGGGTATGCTTCGCGGCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGc                                                    <  6:130783/149‑1 (MQ=255)
                                                                                          ggAGATAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGCCCATg                                               >  3:205300/1‑149 (MQ=255)
                                                                                           gagaTAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGGGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGCCCATgg                                              >  3:109763/1‑149 (MQ=255)
                                                                                           gagaTAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGGGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGCCCATgg                                              >  3:105173/1‑149 (MQ=255)
                                                                                           gagaTAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGCCCATgg                                              >  6:285718/1‑149 (MQ=255)
                                                                                                  aaCACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGCCCATGGTGCAAcc                                       <  8:172833/149‑1 (MQ=255)
                                                                                                       gTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGCCCATGGTGCAACCGGCg                                   >  6:294339/1‑148 (MQ=255)
                                                                                                               ggTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGCCCATGGTGCAACCGGCGTATGgcgc                           <  3:274695/148‑1 (MQ=255)
                                                                                                                 tATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGCCCATGGTGCAACCGGCGTATGGCGCCAg                        >  1:44685/1‑149 (MQ=255)
                                                                                                                     cTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGCCCATGGTGCAACCGGCGTCTGGCGCCAGGAAt                    >  8:136102/1‑149 (MQ=255)
                                                                                                                      ttCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGCCCATGGTGCAACCGGCGTATGGCGCCAGGAAtt                   >  2:307081/1‑149 (MQ=255)
                                                                                                                        cgcgTCCTGGTGGACGGGGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGCCCATGGTGCAACCGGCGTATGGCGCCAGGAAtt                   <  4:105173/147‑1 (MQ=255)
                                                                                                                        cgcgTCCTGGTGGACGGGGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGCCCATGGTGCAACCGGCGTATGGCGCCAGGAAtt                   <  4:109763/147‑1 (MQ=255)
                                                                                                                         gcgTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGCCCATGGTGCAACCGGCGTATGGCGCCAGGAATTGCa                <  5:285718/149‑1 (MQ=255)
                                                                                                                           gTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTaaa                                                                                           >  1:124213/1‑72 (MQ=255)
                                                                                                                           gTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTaaa                                                                                           <  2:124213/72‑1 (MQ=255)
                                                                                                                            tCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGCCCATGGTGCAACCGGCGTATGGCGCCAGGAATTGCAGTg             <  4:302692/149‑1 (MQ=255)
                                                                                                                                 gtggACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGCCCATGGTGCAACCGGCGTATGGCGCCAGGAATTGCAGTgcggc         <  2:13672/148‑1 (MQ=255)
                                                                                                                                  tggACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGCCCATGGTGCAACCGGCGTATGGCGCCAGGAATTGCAGTgcggcg        >  4:151318/1‑148 (MQ=255)
                                                                                                                                    gACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGCCCATGGTGCAACCGGCGTATGGCGCCAGGAATTGCAGTGCGGCGGAt     >  3:82727/1‑149 (MQ=255)
                                                                                                                                       ggCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGCCCATGGTGCAACCGGCGTATgg                              >  2:202249/1‑121 (MQ=255)
                                                                                                                                       ggCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGCCCATGGTGCAACCGGCGTATGGCGCCAGGAATTGCAGTGCGGCGGATTcc  >  1:195220/1‑149 (MQ=255)
                                                                                                                                       ggCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGCCCATGGTGCAACCGGCGTATGGCGCCAGGAATTGCAGTGCGGCGGATTcc  >  1:1686/1‑149 (MQ=255)
                                                                                                                                         |                                                                                                                                                  
GGGAACCGGTTTTGCCAGTGACAGCACCGTTGGTGAACTTCTCGACGTACTCTTCCGAAACGCGCGATGCACGCTCCAGCAGACGGGAGTGGAGATAGAACACGTCGCCTGGGTATGCTTCGCGTCCTGGTGGACGGCGCAGCAGCAGGGAGATCTGACGGTAGGCAACGGCCTGCTTGGACAGGTCATCGTAAACGATCAGTGCGTCTTCACCGCGGTCGCGGAAGAACTCGCCCATGGTGCAACCGGCGTATGGCGCCAGGAATTGCAGTGCGGCGGATTCC  >  NC_002947/6178105‑6178388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑