Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 646,602 C→T 6.9% A75A (GCC→GCT)  acoR → acetoin catabolism regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947646,6020CT 6.9% 171.9 / 2.2 58A75A (GCC→GCT) acoRacetoin catabolism regulatory protein
Reads supporting (aligned to +/- strand):  ref base C (26/28);  new base T (2/2);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

CCACCGCTGATCCTCGATTCATGGCGCCGCTCCATGGATGTGTACCGCCTCGACCCCGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGGCGCACCATCGATTACCGGGTCGAGTCGGCCATCCGCAACGACTGTCGCAAGGCCGGGCTGTATCTGGGGAC  >  NC_002947/646462‑646748
                                                                                                                                            |                                                                                                                                                  
ccaccGCTGATCCTCGATTCATGGCGCCGCTCCATGGATGTGTACCGCCTCGACCCCGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGc                                                                                                                                            >  4:217203/1‑149 (MQ=255)
 caccGCTGATCCTCGATTCATGGCGCCGCTCCATGGATGTGTACCGCCTCGACCCCGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGcc                                                                                                                                           >  5:176812/1‑149 (MQ=255)
   ccGCTGATCCTCGATTCATGGCGCCGCTCCATGGATGTGTACCGCCTCGACCCCGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCTGGCGATGcgca                                                                                                                                         <  6:265248/149‑4 (MQ=255)
   ccGCTGATCCTCGATTCATGGCGCCGCTCCATGGATGTGTACCGCCTCGACCCCGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGt                                                                                                                                         >  2:172993/1‑149 (MQ=255)
    cGCTGATCCTCGATTCATGGCGCCGCTCCATGGATGTGTACCGCCTCGACCCCGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTg                                                                                                                                        <  1:204361/149‑1 (MQ=255)
      cTGATCCTCGATTCATGGCGCCGCTCCATGGATGTGTACCGCCTCGACCCCGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGgc                                                                                                                                      <  7:99133/149‑1 (MQ=255)
      cTGATCCTCGATTCATGGCGCCGCTCCATGGATGTGAACCGCCTCGACCCCGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGgc                                                                                                                                      >  8:99133/1‑149 (MQ=255)
           ccTCGATTCATGGCGCCGCTCCATGGATGTGTACCGCCTCGACCCCGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCt                                                                                                                                 <  3:217203/149‑1 (MQ=255)
            cTCGATTCATGGCGCCGCTCCATGGATGTGTACCGCCTCGACCCCGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCtt                                                                                                                                <  1:309090/149‑1 (MQ=255)
               gATTCATGGCGCCGCTCCATGGATGTGTACCGCCTCGACCCCGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGc                                                                                                                              <  6:106218/148‑1 (MQ=255)
                              tCCATGGATGTGTACCGCCTCGACCCGGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGgc                                                                                                                                      <  3:297595/125‑1 (MQ=255)
                              tCCATGGATGTGTACCGCCTCGACACGGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGgc                                                                                                                                      >  4:297595/1‑125 (MQ=255)
                                cATGGATGTGTACCGCCTCGACCCCGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCgcggcg                                                                                                            <  4:39419/149‑1 (MQ=255)
                                   ggATGTGTACCGCCTCGACCCCGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCt                                                                                                          >  5:159340/1‑148 (MQ=255)
                                     aTGTGTACCGCCTCGACCCCGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGac                                                                                                       >  4:75662/1‑149 (MQ=255)
                                         gtACCGCCTCGACCCCGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTg                                                                                                         >  2:49178/1‑143 (MQ=255)
                                         gtACCGCCTCGACCCCGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTg                                                                                                         <  1:49178/143‑1 (MQ=255)
                                          tACCGCCTCGACCCCGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTg                                                                                                  >  5:234029/1‑149 (MQ=255)
                                          tACCGCCTCGACCCCGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTg                                                                                                  >  2:157864/1‑149 (MQ=255)
                                             cGCCTCGACCCCGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGt                                                                                               <  6:159340/149‑1 (MQ=255)
                                               ccTCGACCCCGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGtgc                                                                                             <  3:59674/149‑1 (MQ=255)
                                               ccTCGACCCCGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGtgc                                                                                             <  3:110635/149‑1 (MQ=255)
                                                     ccccGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTa                                                                                       >  1:145675/1‑149 (MQ=255)
                                                      cccTGTTCCCAGCATTGGCCGCGTATCCTTTCCCTAAGCCTGCTCAACGACTGCCGCGATCGAGCCGTGCTGTTCCTTCGCATCGCCAGCTATGCCGTGGCGCTGTTGCATGAGCGGGTGGGCGGCGCTGACTACTGCGTGCTGCTTAc                                                                                      <  6:187456/149‑1 (MQ=255)
                                                         ggTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGa                                                                                   <  7:83427/149‑1 (MQ=255)
                                                          gTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGAt                                                                                  >  7:31492/1‑149 (MQ=255)
                                                                 gcagGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAgg                                                                           <  1:164105/149‑1 (MQ=255)
                                                                  cagGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAggg                                                                          <  6:176812/149‑1 (MQ=255)
                                                                    ggggCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGgc                                                                        <  1:124905/149‑1 (MQ=255)
                                                                      ggCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGgcgc                                                                      >  7:196392/1‑149 (MQ=255)
                                                                            gTATCCTTTCCCAAATCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGGCGCACCATc                                                                <  6:234029/149‑1 (MQ=255)
                                                                              aTCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGGCGCACCATCGa                                                              >  7:150869/1‑149 (MQ=255)
                                                                               tCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGGCGCACCATCGa                                                              <  1:172993/148‑1 (MQ=255)
                                                                                 cTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGac                                                                                                       <  6:72831/105‑1 (MQ=255)
                                                                                 cTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGGCGCACCATCGATTa                                                           >  4:289978/1‑149 (MQ=255)
                                                                                  tttCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCa                                                                             <  6:313587/130‑1 (MQ=255)
                                                                                  tttCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCa                                                                             >  5:313587/1‑130 (MQ=255)
                                                                                      ccAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGGCGCACCATCGATTACCgg                                                       <  8:31492/148‑1 (MQ=255)
                                                                                       cAAAGCCTGCTCAACGGATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGGCGCACCATCGATTACCGGGt                                                     >  5:299855/1‑149 (MQ=255)
                                                                                       cAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGGCGCACCATCGATTACCGGGt                                                     <  3:75662/149‑1 (MQ=255)
                                                                                        aaaGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGc                                                                                                 <  6:286745/104‑1 (MQ=255)
                                                                                        aaaGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGc                                                                                                 >  5:286745/1‑104 (MQ=255)
                                                                                        aaaGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTAc                                                                                      >  8:214769/1‑115 (MQ=255)
                                                                                        aaaGCCTGCGCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCGAGGGGCGCACCATCGATGACCGGGTc                                                    >  6:222440/1‑149 (MQ=255)
                                                                                                   aaCGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGGCGCACCATCGATTACCGGGTCGAGTCGGCCAt                                         >  8:33223/1‑149 (MQ=255)
                                                                                                   aaCGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGGCGCACCATCGATTACCGGGTCGAGTCGGCCAt                                         >  8:106275/1‑149 (MQ=255)
                                                                                                       aaTGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGGCGCACCATCGATTACCGGGTCGAGTCGGCCATc                                        >  6:235733/1‑146 (MQ=255)
                                                                                                       aaTGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGGCGCACCATCGATTACCGGGTCGAGTCGGCCATc                                        <  5:235733/146‑1 (MQ=255)
                                                                                                                              ttCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGGCGCACCATCGATTACCGGGTCGAGTCGGCCATCCGCAACGACTGTCGCAAGGcc                   <  8:150869/144‑1 (MQ=255)
                                                                                                                              ttCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGGCGCACCATCGATTACCGGGTCGAGTCGGCCATCCGCAACGACTGTCGCAAGGCCggg                <  1:157864/147‑1 (MQ=255)
                                                                                                                              ttCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGGCGCACCATCGATTACCGGGTCGAGTCGGCCATCCGCAACGACTGTCGCAAGGCCGGGCt              >  3:273903/1‑149 (MQ=255)
                                                                                                                              ttCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGAGGCGCACCATCGATTACCGGGTCGAGTCGGCCATc                                        <  4:158251/123‑1 (MQ=255)
                                                                                                                              ttCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGAGGCGCACCATCGATTACCGGGTCGAGTCGGCCATc                                        >  3:158251/1‑123 (MQ=255)
                                                                                                                                 cacggcATCGCTGGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGGCGCACCATCGATTACCgg                                                       >  4:155956/5‑105 (MQ=255)
                                                                                                                                 cacggcATCGCTGGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGGCGCACCATCGATTACCgg                                                       <  3:155956/101‑1 (MQ=255)
                                                                                                                                  acggcATCGCTGGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGGCGCACCATCGATTACCGGGTCGAGTCGGCCATCCGCAACGACTGTCGCAAGGCCGGGCTGTAt          >  1:302733/4‑149 (MQ=255)
                                                                                                                                       aTCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGGCGCACCATCGATTACCGGGTCGAGTCGGCCATCCGCAACGACTGTCGCAAGGCCGGGCTGTATCTggg     >  7:266242/1‑149 (MQ=255)
                                                                                                                                          gCCAGCGATGCCGTTGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGGCGCACCATCGATTACCGGGTCGAGTCGGCCATCCGCAACGACTGTCGCAAGGCCGGGCTGTATCTGGGGAc  <  6:242891/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
CCACCGCTGATCCTCGATTCATGGCGCCGCTCCATGGATGTGTACCGCCTCGACCCCGGTTCCCAGCAGGGGCCGCGTATCCTTTCCCAAAGCCTGCTCAACGAATGCCGCGAACGCGCCGAGCTGTTCCTGCGCATCGCCAGCGATGCCGTGGCGCGCTTGCATGAGCGGGTGCGCGGCGCTGACTACTGCGTGCTGCTTACCGATGCCCAGGGGCGCACCATCGATTACCGGGTCGAGTCGGCCATCCGCAACGACTGTCGCAAGGCCGGGCTGTATCTGGGGAC  >  NC_002947/646462‑646748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑