Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 689,978 A→G 7.0% V220A (GTG→GCG)  PP_0591 ← adenosine deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947689,9780AG 7.0% 165.0 / 2.8 57V220A (GTG→GCG) PP_0591adenosine deaminase
Reads supporting (aligned to +/- strand):  ref base A (28/25);  new base G (2/2);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

ACCACGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTTCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACTTGCTGGGTGGGTGG  >  NC_002947/689836‑690124
                                                                                                                                              |                                                                                                                                                  
accacGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCAt                                                                                                                                              >  1:163948/1‑149 (MQ=255)
  cacGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATgg                                                                                                                                            >  8:262781/1‑149 (MQ=255)
  cacGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATgg                                                                                                                                            >  2:148802/1‑149 (MQ=255)
   acGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGTGCGCGCCAtcga                                                                                                                                           <  1:92102/149‑4 (MQ=255)
           gCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGt                                                                                                                                   <  2:163948/149‑1 (MQ=255)
           gCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCATGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGt                                                                                                                                   >  1:229963/1‑149 (MQ=255)
             aTGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGttt                                                                                                                                 <  2:64447/149‑1 (MQ=255)
             aTGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGttt                                                                                                                                 >  4:211154/1‑149 (MQ=255)
                tCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGa                                                                                                                               <  1:214250/148‑1 (MQ=255)
                tCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGAt                                                                                                                              >  6:218245/1‑149 (MQ=255)
                tCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGAGCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGa                                                                                                                               <  2:63969/148‑1 (MQ=255)
                     gATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATTCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTca                                                                                                                         <  3:211154/149‑1 (MQ=255)
                      aTGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTcag                                                                                                                        <  7:262781/149‑1 (MQ=255)
                             gCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGa                                                                                                                                        >  1:148813/1‑126 (MQ=255)
                             gCTGGCTCATATGGGCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGa                                                                                                                                        <  2:148813/126‑1 (MQ=255)
                               tGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCa                                                                                                                                               <  4:106010/117‑1 (MQ=255)
                               tGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCa                                                                                                                                               >  3:106010/1‑117 (MQ=255)
                                ggCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCg                                                                                                                                         <  8:295052/122‑1 (MQ=255)
                                ggCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCg                                                                                                                                         >  7:295052/1‑122 (MQ=255)
                                   tCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAg                                                                                                               >  7:19234/1‑145 (MQ=255)
                                   tCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAg                                                                                                               <  8:19234/145‑1 (MQ=255)
                                   tCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCt                                                                                                           >  4:227254/1‑149 (MQ=255)
                                            cGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATg                                                                                                  >  1:119745/1‑149 (MQ=255)
                                             gAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGTGCGCGCCAtcg                                                                                                                                            <  6:3926/106‑3 (MQ=255)
                                             gAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGTGCGCGCCAtcg                                                                                                                                            >  5:3926/1‑104 (MQ=255)
                                             gAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATg                                                                                                  <  2:229963/148‑1 (MQ=255)
                                                 acGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTc                                                                                             >  4:201415/1‑149 (MQ=255)
                                                        gCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTcgggcgg                                                                                       <  3:227254/148‑1 (MQ=255)
                                                               gtTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCcttctt                                                                               >  7:140116/1‑149 (MQ=255)
                                                                ttGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCttcttc                                                                              <  5:218245/149‑1 (MQ=255)
                                                                ttGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCAttcttc                                                                              <  2:119745/149‑1 (MQ=255)
                                                                  ggACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTTCGc                                                                            >  4:311072/1‑149 (MQ=255)
                                                                   gACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGttt                                                                                                                                 >  8:188909/1‑95 (MQ=255)
                                                                   gACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGttt                                                                                                                                 <  7:188909/95‑1 (MQ=255)
                                                                   gACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTTCGc                                                                            <  3:59112/148‑1 (MQ=255)
                                                                          ggCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTTCGCCAGCATgg                                                                    <  8:140116/149‑1 (MQ=255)
                                                                           gCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTTCGCCa                                                                          <  1:114031/142‑1 (MQ=255)
                                                                           gCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTTCGCCa                                                                          >  2:114031/1‑142 (MQ=255)
                                                                                          gATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTTCGCCAGCATGGGCAACGGCGACGAAGc                                                    >  7:194081/1‑149 (MQ=255)
                                                                                               gCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTTCGCCAGCATGGGCAACGGCGACGAAGCCTTCg                                               >  3:306992/1‑149 (MQ=255)
                                                                                                cTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGtt                                                                                                                                  <  1:54180/65‑1 (MQ=255)
                                                                                                cTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGtt                                                                                                                                  >  2:54180/1‑65 (MQ=255)
                                                                                                    tcgATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTTCGCCAGc                                                                        >  1:124155/1‑119 (MQ=255)
                                                                                                    acgATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTTCGCCAGc                                                                        <  2:124155/118‑1 (MQ=255)
                                                                                                      gatgatGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTTCGCCAGCATGGGCAACGGCGACGAAGcc                                                   >  7:159071/1‑138 (MQ=255)
                                                                                                      gatgatGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTTCGCCAGCATGGGCAACGGCGACGAAGcc                                                   <  8:159071/138‑1 (MQ=255)
                                                                                                                     cAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTTCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACAccc                         >  5:236357/1‑149 (MQ=255)
                                                                                                                            tCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTTCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGaa                              >  6:182612/1‑137 (MQ=255)
                                                                                                                            tCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTTCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGaa                              <  5:182612/137‑1 (MQ=255)
                                                                                                                              gTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTTCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACt                >  7:179866/1‑149 (MQ=255)
                                                                                                                                ccTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCTAGATGTACTCGGGCGGGCCTTCTTCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACTTg              >  1:12945/1‑149 (MQ=255)
                                                                                                                                ccTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTTCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACTTg              >  5:160800/1‑149 (MQ=255)
                                                                                                                                 gaccaTGGCGTGCGCGCCATCGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCATCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACTTGc             >  3:39678/5‑149 (MQ=255)
                                                                                                                                    gATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTTCGCCAGCATGGGCAATGGCGACGAAGCCTTCGCTACGGGCGCGGTc                                 >  1:158694/1‑126 (MQ=255)
                                                                                                                                    gATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTTCGCCAGCATGGGCAATGGCGACGAAGCCTTCGCTACGGGCGCGGTc                                 <  2:158694/126‑1 (MQ=255)
                                                                                                                                        gcgcgcACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTTCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACTTGCtgggtgg      <  3:311072/149‑1 (MQ=255)
                                                                                                                                            gcACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTTCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACTTGCTGGgtgggtgg  <  8:194081/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
ACCACGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTTCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACTTGCTGGGTGGGTGG  >  NC_002947/689836‑690124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑